細胞毒性抗體 的英文怎麼說
中文拼音 [xìbāodúxìngkàngtǐ]
細胞毒性抗體
英文
cytotoxic antibody- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 毒 : Ⅰ名詞1 (對生物體有害的性質或物質; 毒物) poison; toxin 2 (毒品) drug; narcotics 3 (姓氏) a s...
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 體 : 體構詞成分。
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
- 毒性 : [藥理學] toxicity; virulence; poisonousness毒性測定 toxicity test
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The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本實驗採用spf雞胚及雞胚原代成纖維細胞,從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞,並且這種凝集可以被特異性抗血清所抑制。It was important glucoprotein modulating cell functions by combination to idiosyncrasy receptor on cell membrane, which inducing process of cascade signal magnifying, then passing signals into nucleus to induce the modulation of the serial gene expression and viarous physiological reaction, and inhence immunity and antivirus
人-干擾素( hu - - ifn )是人體因病毒感染或其它誘生劑作用等所產生的一類非特異性抗病毒物質,是調節細胞功能的一類重要的糖蛋白。In one word, the methods for producing acellulace matrix have not been standardized. in this study, we want to select the best way to produce acellular matrix. we also use immunohistochemical method for detecting the magor heterogenetic antigen a - galactosyl residues ( a - gal ) in acellular matrix
並應用免疫組化的方法檢測脫細胞后基質的主要異種抗原,用種植實驗觀察異種機體對脫細胞生物基質的組織反應倩況,另在其上種植鼠成纖維細胞以觀察脫細胞生物基質的細胞毒性和細胞相容問題。Animal health analysis methods - detection of antibodies against classical swine fever by virus neutralisation test and immunochemistry on cell cultures if or ip
動物健康分析方法.病毒中性試驗和細胞培養免疫化學檢測典型豬瘟的抗體Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified
為建立對豬傳染性胃腸炎快速有效的診斷方法,並試圖在預防上提供有效的免疫制劑,本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化;並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。Containing many t cell epi - positions and neutralizing antibody epi - positions, the core protein region and envelope protein 2 of hepatitis c virus ( hcv ) structural protein may induce protective immunity responsion in body
丙型肝炎病毒( hepatitiscvirus , hcv )結構蛋白的核心蛋白( c )和包膜蛋白2 ( e2 )含有多個t細胞表位和中和抗體表位,可誘導機體產生保護性免疫應答。Hi our study, dendritic cells ( dcs ) were derived from the cultivation of peripheral blood monocytes in vitro successfully. then to observe whether dcs transfected with carcinoembryonic antigen ( cea ) - vaccinia recombinant virus ( rv - cea ) can induces cytotoxic t lymphocyte - mediated cea - specific immunity in vitro
體外成功地完成了cd14 +單核細胞來源的樹突狀細胞( dc )的培養,並進一步研究人癌胚抗原重組痘苗病毒( rv - cea )轉染dc后體外誘導的cea特異性細胞免疫。Aati - tumors : the dye lignin has the cytotoxin activeness to the human boby rhinitis cancer ( kb ) cell
抗腫瘤:染料木素對人體鼻炎癌( kb )細胞有細胞毒活性Spf chickens with 21 - day - old were infected subcutaneously with oil - emulsion vaccine of ibdv of germinal or cellcular and injected intramuscularly with different dosages bursin which gain through ultrafilter. lt is proved that bursin of chickens and ducks can both shorten the time of antibody induced against ibdv, raise the level of serum antibody. they make chickens obtaining strong immunocompetente in a short time. agp liters of the group of infecting 0. 4mlcbs + ibdv of germinal and 0. 8mlcbs + ibdv of cellcular or 0. 8mldbs + ibdv of germinal and cellcular are higher than immune control group about 2 liters averagely. the chickens were inoculated with ibdv live vaccine mixed with the different dosages of lyophilized bursin by the eye drop method. the results sugest that cbs or dbs of different dosages can both improve the antibody inducation to different age chickens against ibdv. they may also alleviate the immunological injury of activated virus to bursa of fabricius. and promote the repairation of the lesion. it can be found that bs can raise body weight gain and feed coversion ratio
將超濾獲得的法氏囊活性肽分別以不同劑量肌肉注射21日齡spf雞,同時頸部皮下注射ibd胚毒或細胞毒滅活苗,結果表明:雞、鴨法氏囊活性肽都能夠縮短ibd油苗誘導產生抗體的時間,提高抗體水平,使雞可以在比較短的時間內獲得堅強的免疫。 0 . 4mlcbs胚毒組和0 . 8mlcbs細胞毒組或0 . 8mldbs胚毒和細胞毒組的agp抗體滴度平均比免疫對照組高2個滴度。將法氏囊活性肽與ibd活苗聯合免疫雞,結果表明:不同劑量的cbs和dbs都可以對不同日齡雞ibd抗體的產生有不同程度的促進作用;還可以減少弱毒對雞法氏囊組織的損傷,加快其修復。Functions : this product contains “ antifungal active protein ” with optimal and strong sterilization effect, which could strengthen the immunity of body fluid, activate the macrophage, strengthen the phagocytosis ability of macrophage, strengthen the body immunity, restrain the growth, pervasion and transfer of abnormal cells, the product has strong sterilization effect and could strengthen the disease resistance ; it is remarkably effective in improving the immunity and improving the infirm constitution of pets ; the amino acid content and composition in the product are moderate and rational, with the characteristics of strong palatability, nutrition balance and immune element abundance, etc
功能:本產品中擁有極佳強烈殺菌作用的「抗菌活性蛋白」 ,能增強體液免疫功能,活化巨噬細胞,增強其吞噬能力,可增強機體免疫力,抑制非正常細胞生長、擴散和轉移,具有強烈的殺毒作用,增強抗病性;對提高寵物免疫力,改善虛弱體質有顯著效果;其中氨基酸含量適中、組成合理,具有適口性強、營養均衡和免疫物質豐富等特點。Ricin toxin has wide - range anti - tumor spectrum. however, because rta has no selectivity, it can damge normal ce lls too. these years, ricin and it ' s a chain ( rta ) have been used to conjugate with monoclonal antibody to prepare immunotoxins ( its )
近年來,蓖麻毒a鏈可以與單克隆抗體相交聯而制備免疫毒素( immunotoxin , it ) ,免疫毒素既具有強大的腫瘤殺傷作用,同時又有抗體的特異性,可以將毒素分子靶向運送到腫瘤細胞,以減少對正常細胞的損傷。Elicitors molecules that initiate cell signaling pathways leading to the activation of plant defense genes and the production of phytoalexins and other defensive chemicals in response to infection by a pathogen
誘導子:可以引發細胞信號通路的分子,使植物防禦基因、植物抗毒素及其他防禦性化學物質製品被活化以防病原體的感染。Mutated plasmid was transformed into e. coli tg1 cells to produce engineered peptide, then the peptide was purified by cm sepharose ion - exchange column. in vitro bactericidal assay and drug withdrawal were used to identify the bioactivity of the engineered peptide. the planar lipid bilayer membrane was used to assay the electrophysiology of the engineered peptide. toxicity studies on mammalian cells were used to assay the toxicity of the engineered peptide
將重組質粒轉化入大腸桿菌tgi工程菌中,生產構建的工程多膚,離子交換純化后獲得工程多膚初步純化產物,體外抗菌試驗、藥物撤離試驗檢測工程多膚的抗菌活性,在人工脂質膜上測定其形成離子通道的特性以初步研究抗菌機理, ?並觀察其對真核細胞的毒性作用。No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
將重組病毒rbmbacph - egf以10moi感染bmn細胞, 72小時后收集培養細胞和上清;培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物,粗略估計表達量約6 7 g 2 10 ~ 6個細胞(相當于egf標準品) ;重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲,每隔24h收集蠶血淋巴,經elisa檢測發現第4天表達量最高,根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ; elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。The expression of ha in vero cells infected with rprv - ha was detected by western - blot. the results indicated that ha protein could be consistently detected from a serial passages of vero cells infected with rprv - ha. the recombinant virus can be further developed as a live vectored vaccine against pseudorabies and swine influenza
結果表明所獲得的重組病毒( rprvha )遺傳性狀穩定,在培養細胞中能穩定的表達與sivha具有相似生物學活性的外源蛋白,為進一步制備抗豬流感的重組偽狂犬病毒活載體疫苗奠定了基礎。The originalities of this paper are : 1, development of a a flow cytometry - based assay for quantitative analysis of cellular proliferation and cytotoxicity in vitro ; 2, the soluble secretion of k. 562 cell lines reduce the number of pbmc, but promote the activity of pbmc in dose - dependent manner ; 3, soluble secretion of k562 cell lines can induce the no production by pbmc, but no only plays a part of the role of soluble secretion of k562 cell lines ; 4, establishing a in vitro model and giving some parameters for sreening and appraising anti - tumor medicine
本研究的創新點在於: l 、建立了用流式細胞術定量測定細胞增殖和細胞毒性的直接檢測技術; 2發現k562可溶性分泌物劑量依賴性地使pbmc細胞數量減少但活性增加; 3 、 k562可溶性分泌物能誘導pbmc產生no ,但是no的作用並不等同於腫瘤上清的全部作用。 4 、為抗腫瘤藥物篩選提供了一個體外模型,並明確了一些篩選指標。Results only 76 people with blood antibody titer > 1 : 5 had the cellular immune effect ( the particular plt was marc than 5 % ), 35 people without antibody and 4 people whose blood antibody titer was 1 : 5, nobody had the cellular immune effect
結果狂犬病疫苗接種后,抗體效價達到大於1 : 5的100人中, 76人同時產生細胞免疫力(即狂犬病毒特異性淋巴細胞轉化率大於5 % ) ;而抗體效價等於1 : 5的4人和未產生抗體的35人中,無人產生細胞免疫力。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。Lee, ys ; yovn, sj. immune response induced by immunization with hepatitis virus core dna isolated from chronic active hepatitis patients j. immunollett. 2001 , 78 ( 1 ) : 1320
賈戰生,慢性乙型肝炎病毒攜帶者外周單個核細胞體外抗原誘導的抗體反應j .第四軍醫大學學報, 2000 , 20 ( 7 ) : 4547分享友人