細胞轉染 的英文怎麼說
中文拼音 [xìbāozhuǎnrǎn]
細胞轉染
英文
cell transfection- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 轉 : 轉構詞成分。
- 染 : Ⅰ動詞1 (用染料著色)dye 2 (感染) catch [contract] (a disease) 3 (沾染) acquire (a bad hab...
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的:構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后,利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素,初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。Stable suppression of afp gel expression by rnai in smmc - 7721 cells
表達質粒穩定轉染肝癌細胞克隆的構建Trial 2, effect of supplemental copper of different type on nutrition and specific immunity of mice - ii the grouping of trialt animal was the same as trial 1, at the first day, second day, third day, one mouse was injected with pha brine fluid for 10mg / kg avoirdupois after weighing in the same time in each repeat, following the 7d, 14d, 21d, 8d feeding period, after weighing, blood was made, wrigh - giemsa coloration, counting the number of lymphocyte female cell and overage lymphocyte, index of immune organ, copper concentration in liver and spleen
試驗二,不同形式銅對小鼠營養與特異性免疫功能的作用-試驗動物分組同試驗一,進入正式試驗期后,在每周第1天、 2天、 3天同一時間每重復選取1隻小鼠,稱重后每天按10mg kg體重肌肉注射一次植物血凝素生理鹽水溶液,並於試驗第7天、 14天、 21天、 28天稱重后尾尖取血,姬姆薩-瑞氏染色,計算t淋巴細胞轉化率,計算免疫器官指數,測定肝臟、脾臟銅含量。The protective effect of bcl - 2 gene transfection on heat stressed cardiomyocytes
2基因轉染對熱應激心肌細胞的保護作用To investigate the consequence of this interaction, aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector. confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus
在構建了紅色熒光蛋白aes表達載體后,將其與tle綠色熒尤蛋白載體共轉染細胞,共聚焦顯微鏡觀察發現這兩種分子在胞漿中有共存現象,而且aes的表達可抑制tlei向胞核內的聚積。17 - hsd8 / pcdna3. 1 recombinant protein expressed in hek 293 cells and it can slightly interconvert estrone to estradiol. moreover, it can catalyze the conversion from testosterone to androstenedione. however, 17 - hsd3 did n ' t show any conversion to all the substrates checked in this study
而17p一hsds轉染hek293細胞表達的17p一hsds / pcdna3 . 1重組蛋白能微弱地催化el和ez之間的相互轉化,同時也能催化從t到a的氧化反應。Protective effect of c - fos antisense gene recombinant transfection on ischemic hypoxic cardiomyocytes
反義基因轉染對缺血缺氧心肌細胞的保護作用We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene, 2. 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3. 0
我們直接將含有4個內含子和自身信號肽的2 . 4kb全長人生長激素基因直接克隆至真核表達載體pcdna3 . 0 ,然後利用脂質體轉染家蠶bmn細胞,瞬時表達hgh 。The primers were in the sequence of vector pegfp - 1 outside the lox sites, and the whole sequence of the vector were transfected into the fibroblast cells
引物設計的位置是在loxp和lox511之外的載體序列,整個載體序列都隨轉染而進入細胞。The effectiveness of the nuclear localization signal selected was clearly tested in initial experiments. based on this, the co - transfection systems used in the study was established. we first observed the multinucleate cells and chromatin bridges in cultured hela cells when the cells are marked with gfp and hochest33342, after co - transfection with the gfp expression plasmids and rnai plasmids rhe or rhc
當分別共轉染附加kozac序列和核定位信號的gfp與人集縮素smc亞基hcap一e特異的rnai質粒rhe和人集縮素smc亞基hca屍一c的f { nai質粒rhc時,都觀察到gfp和hochest33342標記的轉染后hela細胞表現多核和染色質橋現象。The results of these early research work showed that rna polymerase iii transcription was localized in the nucleoplasm. however, with the development and the application of new technologies since 1990s, the controversy arose on the transcription sites of rna polymerase iii. in recent years, more and more scientists presumed that rna polymerase iii transcription might not occur in the nucleoplasm but in the nucleoli
自上個世紀八十年代初期,人們相繼運用細胞化學染色、電鏡放射自顯影等進行研究的結果表明: rna聚合酶的轉錄發生在核質中,但隨著新的研究技術的發展和應用,人們卻發現rna聚合酶的轉錄可能發生在核仁中,從而對早期的研究結果提出了質疑。Transfection efficiency comparison of cationic liposome - dna complexes and lipid - protamine - dna complexes in vitro
復合物體外細胞轉染率比較Infectious agents such as human papillomavirus can lead to cellular transformation as well
感染性因素如人類乳頭狀瘤病毒能導致細胞轉化。Future strategy and puzzles of heavy ion beam mediated technique in genetic improvement of biological bodies
重離子輻射提高腺病毒介導小鼠黑色素瘤細胞轉染率的研究Cytomixis in pollen mother cells usually occured at prophase of meiosis i, but this phenomenon was observed at other stages occasionally
花粉母細胞間染色質穿壁轉移大多發生在減數分裂的前期聯會時期,偶爾也有在其他時期發生的。We propose that genetic materials in rootstock being translocated and integrated into the genome of the germ cells and embryonic cells in scion are the main reasons why the majority of the hybrid seedlings have wild properties and the heredity of fruit trees violate mendel ' s laws of heredity
砧木中的遺傳物質轉移並整合到接穗生殖細胞和胚胎細胞的染色體組中,是雜種後代出現大量野生植株和果樹遺傳不遵守孟德爾定律的主要原因。The endocytosed albumin appears to undergo transcytosis in large esicles ( 500 nm in diameter ), identified by immunogold staining of endogenous albumin by electron microscopy, to the basolateral membrane where the albumin is disgorged back to the peritubular blood supply
對內源性白蛋白進行免疫金染色然後在電鏡下觀察發現:這些重吸收的白蛋白以大的囊泡(直徑500納米)形式被跨細胞轉運到腎小管基底膜側,然後回吐到腎小管周血管。Determined by immunohistochemical technique, there were hcv structural proteins in both sp2 / 0 - hcv and its transplanting tumor tissue
建立了hcv結構基囚體外細胞轉染系spyo ncv ,爿。To study the biological function of sh2a gene, we constructed its recombinant expression vec - tor, and investigated its function by cell transfection, kinase assay, subcellular localization and expression analysis. materials and methods 1
我們構建了sh _ 2a基因的真核重組表達載體,通過細胞轉染、激酶活性檢測、亞細胞定位、表達分析和流式細胞儀等方法對其功能進行了初步的研究。By cell transfection and kinase assay, we found that sh2a may be an inhibitor and play an important role in some key step of pkc signal transduction
二一sh ,經過細胞轉染和激酶活性檢測,結果提示, sh基因很可能作為一種抑制因子,在pkc信號轉導通路中某些關鍵環節起著調節作用。分享友人