細菌蛋白酶 的英文怎麼說

中文拼音 [xìjūndànbái]
細菌蛋白酶 英文
bacteria protease
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 細菌 : germ; bacterium （pl. bacteria）; fungus （pl. fungi）
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. After careful studying their relative importance to immune response and the possibility of the match, seventeen sequences of interest were selected for further experiment, including estss analogous to 11. 5kd antibacterial peptide, lysozyme, serine protease and its inhibitor, lectin, antifreeze protein, et al. primers designed according to the sequences were used to amplify the corresponding estss from both blood and cephalothorax cdna library

    在仔細分析了它們在免疫系統中的重要性和在對蝦中出現的可能性之後，從中選出了17條可能編碼抗菌肽，溶菌酶，凝集素、絲氨酸蛋白酶及其抑制劑，抗凍蛋白等蛋白質的序列，以此為依據設計引物，在中國對蝦的血液和頭胸部cdna文庫中擴增相應的序列。
  3. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  4. In the stomach, this acid functions to kill bacteria in foods, to soften foods and to convert the inactive enzyme pepsinate into its active from pepsin, to begin the digestion of protein

    在胃裡，這種酸的作用是殺司食品的細菌，使食物軟化，把鈍化的胃蛋白酶原轉化為有活性的能消化蛋白質的胃蛋白酶。
  5. The results showed : protamine could inhibit the growth of " bacillus subtilis " without destroying the cellular wall and significantly inhibit the activities of succinate dehydrogenase and malata dehydrogenase

    試驗結果表明：鯉魚魚精蛋白對枯草芽袍桿菌具有較強的抑制作用，但對枯草芽抱桿菌細胞壁不產生破壞作用；對黑曲?胞內的琥珀酸脫氫酶和蘋果酸脫氫酶的活性具有明顯的抑制作用。
  6. Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on

    該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。
  7. Study on the ph value on the growth of the halophilic bacteria in the pickled food

    一株產低溫堿性蛋白酶嗜冷海洋細菌
  8. Increased skin surface activity, the metabolism of trace elements regulate cell growth. enhance the vitality of elastin and collagen protein, preventing cell aging and promote cell regeneration and increase blood circulation. improving metabolism, anti - bacterial, anti - itching … … the aging of the skin, relax, bark, diaopi, have had some effect

    增加皮膚表面酶活性，調節細胞生長新陳代謝所需的多種微量元素，提高彈性蛋白與膠原蛋白的活力，具有防止細胞老化，促進細胞再生，增加血液循環，提高新陳代謝，抗菌，止癢… …對皮膚老化，鬆弛，死皮，掉皮，有一定作用。
  9. Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods

    大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。
  10. The number of 3 rhizosphere microorganisms ( bacteria, fungi, actinomycete ) and 5 enzyme ( catalase, protease, urease, phosphatase, invertase ) activities were studied during the whole life of corn plant in sandy loam, loam and clay soil textures. [ method ] using yedan22, the number of 3 rhizosphere microorganisms and 5 enzyme activities with different textural soils were investigated in a pond

    摘要目的明確不同質地土壤（砂壤、中壤、重壤）玉米生育期間根際微生物（細菌、放線菌、真菌）數量與酶（脲酶、蛋白酶、磷酸酶、轉化酶、過氧化氫酶）活性的變化。
  11. [ objective ] the number of 3 rhizosphere microorganisms ( bacteria, fungi, actinomycete ) and 5 enzyme ( catalase, protease, urease, phosphatase, invertase ) activities were studied during the whole life of corn plant in sandy loam, loam and clay soil textures. [ method ] using yedan22, the number of 3 rhizosphere microorganisms and 5 enzyme activities with different textural soils were investigated in a pond

    摘要目的明確不同質地土壤（砂壤、中壤、重壤）玉米生育期間根際微生物（細菌、放線菌、真菌）數量與酶（脲酶、蛋白酶、磷酸酶、轉化酶、過氧化氫酶）活性的變化。
  12. After pcr checking and electro - transformation plasmid from c. elegans into dh5a, isolating plasmid from dhsct, digesting them with restriction enzymes and dna sequencing, six cdna fragments, which protein products can interact with rapgap, from cdna library were got

    將pcr擴增陽性及或lacz強陽性質粒電轉化入dhsa細菌，提取質粒后酶切、測序。發現有6個來源於celegqnscdna文庫的dna片段編碼的蛋白可以與ra帥ap相互作用，其中兩個為rapgap 。
  13. In this study, a new approach of plant virus - resistance was expored by making up the cdna of ppiv. nib gene was amplified from plasmid pysr, which is plant expression vector containing potyvirus y nib gene. the nib gene was fused with the fragment encoding the mature part of papaya proteinase iv

    將番木瓜蛋白酶的cdna序列以全長、去除信號肽部分和成熟酶部分分別連接到細菌表達載體中，表達結果表明，只有含去除信號肽部分cdna的載體才能檢測到相應的表達的蛋白條帶。
  14. The protein designated as phls is highly homology to a phospholipase accessory protein, and is supposed to play important role in phospholipase ai expression. the phospholipase activity blotting of sds - page gel showed that serratia marcescens cw - w - 90 - 3 produce several phospholipases : p60 p33

    Phls與多種細菌產塵的磷酯酶a _ 1的輔助蛋白（ accessoryprotein ）有很高的同源性，它可能在磷酯酶a _ 1的表達過程中起重要作用。
  15. Exposure of cells to the fungal inhibitor wortmannin eliminated focus formation by all repair factors examined, suggesting a role for the phosphoinositide ( pi ) - 3 family of protein kinases in mediating this response

    抗真菌藥物渥曼青霉素作用於細胞，能通過檢測到的修復因子消除形成的病灶，這說明了蛋白激酶中磷酸肌醇家族在這一反映中所起的作用
  16. To date there is no specific database for toxin and anti - nutrient proteins. in order to establish such a database, we have collected data from some nucleotide and protein database available at present. totally, 1033 toxin proteins, including 172 from plants, 251 from animals, 577 from bacteria and 42 from " other organisms, as well as 1013 lectins and 391 proteinase inhibitors are collected

    本文通過對主要基因或蛋白數據庫進行檢索，收集散落於不同基因或蛋白數據庫中的毒蛋白氨基酸序列數據1033個，其中植物毒蛋白172個，動物毒蛋白251個，細菌毒蛋白557個，其它生物如真菌、藻類等的毒蛋白42個；抗營養因子蛋白數據1404個，其中凝集素1013個，蛋白酶抑制劑391個。
  17. In order to get some functional clues from their structures, the upstream regulation region of ndrgl gene and second structure of ndrg2 protein are performed bioinformatics analysis ; we found that there are several binding sequences of some diffirent transcription factors, their functions include regulating tissue - specific gene expression, regulating expression of genes related to growth and early development of cells, besides this, regulating expression of genes under some stimulated conditions, and so on. predict in protein fold classification shows that ndrg2 belongs to alpha / beta hydrolase fold family, and there are high similarity between ndrg2 and epoxide hydrolase from bacteria, this suggests that ndrg2 protein may has enzymatic functions associated with resisting the oxidative stress, maintaining the balance of cell redox potential, involving in the metabolism process of xenobiotics or intracellular toxic molecules

    研究發現呷基因的調控區存在多種轉錄因子結合位點，功能主要涉及組織特異性表達調控，細胞生長發育相關基因的表達調控，刺激反應基因的表達調控等； ndrgz蛋白在結構上屬于a小水解酶類折疊，折疊分類預測表明ndrg2與其中的的細菌環氧化物水解酶的二級結構極為相似，提示ndrgz蛋白具有一定酶活性，可能參與細胞抗氧化應激反應，維持細， an ） armtbffiofbfochmilsyn ） mdafblechmrbfobo4第四軍醫大學碩士學位論文胞內氧還電勢平衡，參與內外源有毒物質的代謝等。
  18. Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme

    作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切，電泳回收目的片段，將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中，轉化大腸桿菌tgi感受態細胞，得到的轉化子經pcr鑒定和酶切分析，篩選出符合閱讀框的重組子，構建成重組表達質粒pet - hn ，並在大腸桿菌bl _ （ 21 ） （ de _ 3 ）宿主菌中成功地表達了含目的蛋白的融合蛋白，融合蛋白的分子量約66kd ，加入iptg誘導8h后，蛋白表達幾乎達到最高水平。
  19. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  20. At first, the vrl - cdna plasmids were transformed into tg1 r. coli to enlarge vrl - cdna ; then the plasmids were extracted and cut off with enzymes ; subsequently, vrl - cdnas were transcribed into vrl - inrna by t7 or sp6 polymerase in vitro, following vrl - mrnas were injected into xenopus laevis oocyte to express into vr1 receptor protein ; at the end the vrl + - oocytes were tested by double electode voltage clamp

    這項實驗中，我們首先將大白鼠vr1 - cdna質粒轉入大腸桿菌中進行擴增，然後提取質粒並酶切，然後在體外轉錄成vr1 - mrna ，接著將vr1 - mrna注射到非洲爪蟾卵母細胞中表達為蛋白質受體，建立了實驗模型細胞，最後用雙微電極電壓鉗檢測此模型細胞。
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