細菌蛋白 的英文怎麼說
中文拼音 [xìjūndànbái]
細菌蛋白
英文
bacterial protein- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 細菌 : germ; bacterium (pl. bacteria); fungus (pl. fungi)
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。In bacterial systems transport activities are performed by membrane proteins.
在細菌系統中運輸活性是通過膜蛋白實現的。Two protein peaks can be obtained by bio - gel p - 6 chromatography and both peaks have antimicrobial activity. so the bacteriocin is consisted of two proteins with different mw. only one protein with larger mw can be detected through tricine - sds - page, and its mw is about 8, 570da
採用30硫酸銨就能完全把發酵液中的細菌素全部沉澱,通過生物膠bio - gelp - 6層析發現細菌素被分離出兩條抗菌蛋白峰,這表明r21 - 4產生的細菌素是由兩種不同分子量的蛋白質組成的,通過tricine - sds - page檢測,只能檢測到一條分子量相對較大的細菌素,分子量在8 , 570da左右。Now, in a study that took more than five years to complete, rockefeller university researchers, in collaboration with a team of bacteriologists at the university of wisconsin, madison, have become the first to solve the structure of a protein complex that protects these cells from singlet oxygen
現在,洛克菲勒大學的研究人員與威斯康星大學的細菌學家一同協作,歷經5年多時間的研究,首次獲得了一種能夠保護細胞免受單態氧損傷的蛋白復合體的晶體結構。Isolate all grew well in the culture medium with initial ph 4 - 10, the optimal growth temperature range was from 28 to 30. 5 degree c. it grew well on the medium for fungi growth, such as pda medium and czpek medium etc, and also grew well on the cause ' s i medium and the non - nitrogen medium, but little growth on the luria bertani medium ( lb ) and beef extract peptone medium. it did not need special nutrition factors for growth, but source of the carbon was the key factor to growth, all of its nutrition needs were different from that of common bacteria
該菌在初始ph4 - 10的培養基中都能夠生長,生長最適溫度范圍為28 - 30 . 5 ,在pda 、查氏等真菌培養基中生長旺盛,在高氏1號和無氮源培養基中同樣生長良好,而在lb與牛肉膏蛋白腖等細菌培養基中生長很差,碳源是其生長的關鍵因子,這有別於一般細菌的營養需求。They identified two bacteria, lactobacillus casei and lactobacillus plantarum, which can be added to beans so they cause minimal distress to those who eat them, and to those around the bean - lovers, marisela granito of simon bolivar university in caracas, venezuela and colleagues reported
研究人員發現,把兩種細菌酪蛋白乳酸桿菌lactobacillus casei和植物乳酸桿菌lactobacillus plantarum添加到豆子中,就可以讓其產生氣體的可能性變小。這樣豆子愛好者們和他們身邊的人就都能從中受益。After careful studying their relative importance to immune response and the possibility of the match, seventeen sequences of interest were selected for further experiment, including estss analogous to 11. 5kd antibacterial peptide, lysozyme, serine protease and its inhibitor, lectin, antifreeze protein, et al. primers designed according to the sequences were used to amplify the corresponding estss from both blood and cephalothorax cdna library
在仔細分析了它們在免疫系統中的重要性和在對蝦中出現的可能性之後,從中選出了17條可能編碼抗菌肽,溶菌酶,凝集素、絲氨酸蛋白酶及其抑制劑,抗凍蛋白等蛋白質的序列,以此為依據設計引物,在中國對蝦的血液和頭胸部cdna文庫中擴增相應的序列。Acid production of lactic acid bacteria is sufficient to coagulate milk proteins.
乳酸細菌產生的酸足以使乳蛋白質凝固。However bacteria ftsz has some cognation relationship with tubulin, perhaps they have the same ancestor
細菌界中ftsz還與tubulin蛋白具有較近的親緣關系,推測起源於共同的祖先。Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into
經限制酶消化和dna序列分析,證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列,在iptg誘導下兩種嵌合分子都獲得了分泌表達,表達產物主要集中在細胞周質空間。Effect of antibactrial proteins of musca domestica larvae on a
家蠅幼蟲抗菌蛋白對黑色素瘤細胞的影響Advances in helicobacter pylori neutrophil - activating protein
幽門螺桿菌中性白細胞激活蛋白的研究進展Cloning and expression of neutrophil activating protein gene of helicobacter pylori
幽門螺桿菌中性粒細胞激活蛋白的基因克隆與表達Geotrichum candidum, candida mycoderma and rhizopus were used as seed speices respectively and daqu distiller ' s grains used as main raw materials ( addition of adequate auxiliary materials as culture medium ) to produce single cell protein by solid fermentation
摘要以白地霉、假絲酵母和根霉為菌種,以大麴丟糟為主要原料,通過添加適當輔料為培養基,經固態發酵方法生產單細胞蛋白。This can now be incorporated into proteins made by the bacterium
這一做法可以用在細菌蛋白質中。Doxycycline gums up the assembly line on which proteins are made, whereas ciprofloxacin stops the dna message about how to make each protein being read in the first place, so there is plenty of scope for interference between the two
脫氧土霉素是通過黏附在蛋白質生產線來阻止細菌蛋白質合成的,而環丙沙星是通過阻抑指導蛋白合成的dna遺傳信息的讀取過程來祈禱抗菌作用的。These proteins are ubiquitous in the plant kingdom where they form a multiple genetic family. they have been suggested to be involved in dif ferent aspects of plant physiology and cell biology through their ability to bind and / or carry lipophilic compounds, including the formation of cutin by transporting the hydrophobic cutin monomers to the apoplast and the defence of plants against pathogens as antimicrobial agents and in flowering
此外nsltp在植物體內廣泛存在並由一個基因家族編碼,由於它能結合和轉運脂類物質,因此推測它可能參與植物體內許多不同的生理過程,如轉運蠟質的角質單體到表皮細胞外側,作為抑菌蛋白抵抗病原菌的入侵以及花粉和柱頭的識別等。The sds - page electropheresis of whole - cell proteins was applied in classification of 71 strains isolated from astragalus spp. it was showed that the technique is a simple and rapid method in classification of rhizobia. the similarity of strains in the same group is 78 %, and dna homology is above 70 %
採用sds page技術對71株黃芪根瘤菌進行了全細胞蛋白的聚類分析.結果表明,這是進行根瘤菌分類時一種簡便快速的分群方法,分群的菌株相似性水平為78 ,群內菌株的dna同源性70The mrna and protein expression were assayed by rt - pcr and sds - page. the results were found that the specific 740bp dna bases of il - 6 was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 49 kda which is consisted of a 23 kda protein deduced from the il - 6 gene sequence and gst ( 26 kda )
以iptg誘導融合蛋白表達,經rt - pcr檢測發現740bp的特異性il - 6條帶;通過sds - page分析,在49kd處出現gst ? pil6融合蛋白條帶,其表達量占總細菌蛋白量的30 ,證明豬il - 6基因得到了正確轉錄和表達。分享友人