細菌重組 的英文怎麼說

中文拼音 [xìjūnzhòngzǔ]
細菌重組 英文
bacterial recombinant
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 細菌 : germ; bacterium （pl. bacteria）; fungus （pl. fungi）
  • 重組 : bpr
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Mr. m answers : " it is said that there are 300 or 400 kinds of oral microorganisms. they multiply and cause not only halitosis but also gums bleeding, gingivitis, periodontits, tooth decay and oral ulcer. when it becomes serious it can lead to pulpitis, periapical periodontitis and cellulitis

    M先生答：因為口腔內寄生微生物種類繁多，據說有三四百種，這些細菌大量繁衍除了能引起口臭外，局部發病可導致牙出血牙炎牙周炎病蛀牙口腔潰瘍等嚴重時會導致牙髓炎牙根尖周炎蜂窩組織炎等。
  3. The cause that causes dark skiing ulcer has a lot of, have endocrine, food, genetic factor, the one mite bug that returning bug having mite also is the main reason that causes dark skiing ulcer lives inside wool bursa and sebaceous glands, it absorbs the nutrition of wool bursa and sebaceous glands cell, discrepancy skin carries a lot of bacteria, it secretes the dead damage body of excretive material and mite bug to be able to cause the skin organize inflammation to cause dark skiing ulcer

    引起暗瘡的原因有很多，有內分泌、飲食、遺傳的因素，還有蟎蟲也是引起暗瘡的重要原因之一蟎蟲生活在毛囊和皮脂腺內，它吸收毛囊和皮脂腺細胞的營養，出入皮膚帶入很多細菌，它分泌排泄的物質和蟎蟲的死亡殘體都會造成皮膚組織發炎引起暗瘡。
  4. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  5. Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted, and the target proteins expressed by iptg induced in escherichia coli. as well as in eukaryocyte ( hepg2 and cos - 7 ), then their antigenity were detected

    結論：截短的乙型肝炎表面抗原分子的原核和真核表達』重組質粒成功被構建及分別在人腸桿菌efl得到誘導表達和存貞核細胞ifj表達，並檢測劍其表達產物的抗原特性。
  6. The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid

    將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。
  7. Ammonia - oxidizing bacteria which oxidize ammonia to nitrite is a key group of nitrifying bacteria. the population of ammonia - oxidizing bacteria is variable with the different environment

    將氨氧化為亞硝酸鹽的氨氧化細菌是硝化菌群的重要組成部分，它的種類隨生境差異而有所不同。
  8. The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21

    本研究將已克隆的真菌細胞色素p450nor基因插入原核表達質粒載體prset和pet28的bamhi / hind位點，成功構建重組表達質粒prt - p450nor和pet - p450nor ，並轉化到e . colibl21 。
  9. Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch

    利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。
  10. Some success in interferon production by bacteria has recently been achieved by the recombinant dna technique.

    近年曾經應用重組DNA技術使細菌產生干擾素獲得了一定成效。
  11. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  12. After the protein was electrophoresised and purified, the protein activity was detected by elisa, the protein activity of vp1 is higher than vp0 vp3. at last, the activity of vp1 made in our lab was detected with the agentia made in our lab

    將陽性重組子轉化到大腸桿菌er2566細菌內，用ipig進行誘導表達蛋白，蛋白經電泳、純化，然後用elisa方法檢測蛋白活性， vp1蛋白活性相對高於vp0 、 vp3 。
  13. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  14. The results of archaeal diversity analysis showed that most of the members belonged to crenarchaeota, and included many unidentified archaea species. abundant bacteria related to the metabolism of sulfur and methane were found in sediment collected from " warm pool " area, which indicated that the metabolism of sulfur and methane played an important role in the substance and energy conversion of this area

    在「暖池」區沉積物中發現了豐富的與硫及甲烷代謝相關的細菌，對這些細菌的種類組成及其在各層次沉積物中的分佈、數量進行了分析，發現該海區沉積物中存在一條完整的硫和甲烷代謝循環途徑，它們的代謝在物質能量代謝中占據重要的地位。
  15. Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on

    該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。
  16. Studies of the crystal structure of endostatin have shown a compact globular fold, with one face particularly rich in arginine residues acting as a heparin - binding epitope, this site was recently shown to be involved in the inhibition of induced angiogenesis. experimental studies show that recombinant endostatin specifically inhibits the proliferation of endothelial cells in a dosedependent fashion. recombinant endostatin from bacteria is largely insoluble, but still efficient in arresting tu mor growth after injection into mice. intermittent therapy with recombinant bacterially produced endostatin reduces several experimental tumors, including lewis lung carcinoma, to a dormant state. no sign of drug induced resistance has been reported and, in the original study, the treatment dormancy appeared to persist even when therapy was discontinued. sowe regard endostatin as a promising anti - tumor drug

    許多研究表明重組內皮抑素特異性抑制內皮細胞增殖，而且這種抑制作用呈劑量依賴性。細菌表達產物內皮抑素大部分以不溶形式存在，將這種混懸液注射治療老鼠仍可以抑制腫瘤生長。于小鼠皮下重復注射內皮抑素重組蛋白，幾乎完全抑制鼠lewis肺癌等多種腫瘤生長，並無耐藥性產生，即使中斷治療腫瘤也不再復發。
  17. The results of carp ' s growth performance indicated : the mixed nutrient solution of psb could improve the increase of carp ' s weight than other three groups ( carp ' s diet of juxing, flavomycin, " yixubao " ), they are 9. 5 %, 9. 6 %, 15. 4 % respectively. and it could decrease the feed consumed / gain of carps than others, they are 2. 6 %, 6. 3 %, 4. 8 % respectively. all those showed that the mixed nutrient solution of psb could enhance the growth performance of carp

    光合細菌復合培養物作為飼料添加劑應用於鯉魚生產試驗，在飼喂35天後試驗組總增重分別比某公司魚飼料組、添加黃黴素組（ 3ppm ） 、 「益畜寶」組提高9 . 5 、 9 . 6 、 15 . 4 ，料肉比降低了2 . 6 、 6 . 3 、 4 . 8 ，說明光合細菌可以提高鯉魚的生產性能。
  18. The research interests of this group include : aborvirus diagnosis technology development and the interaction of aborvirus and mosquitoes, entomopathogenic bacteria and insecticidal gene resources, microbial genomics and comparative genomics, insecticidal proteins and their mode of action, construction of engineering strains with higher toxicity and wider active spectrum, production, standardization and the application of bio - pesticide and other microbial agents, resistance mechanism in target insects and the resistance management

    重點研究登革熱病毒、乙型腦炎病毒和西尼羅病毒的快速檢測及病毒與宿主的相互作用關系，蚊蟲病原微生物菌種及其基因資源，微生物基因組學和比較基因組學，殺蚊毒素蛋白特性和作用方式、殺蚊細菌的遺傳改良和工程菌株的構建，新型細菌殺蚊制劑的研製及野生型和重組微生物對環境的安全性評估等，發展新的生物防治技術，建立和完善以生物防治為主的蟲媒病毒媒介蚊蟲綜合防治體系。
  19. The cultured cell suspensions tested by western - blotting showed that transfected cells could express the exogenous gene and secrete human lactoferrin protein, with mw of 34 kd. the highest amount detected with elisa reached 65mg / l medium / 105 cells. the recombinant hlf protein has the effect of inhibiting e. coli proliferation, whose activity is 1. 4 - 1. 8 times higher than the commercially available hlf

    誘導后，培養液上清通過western - blotting分析證明，轉染細胞表達並分泌出人乳鐵蛋白，分子量為34kd ； elisa檢測重組蛋白最高表達量為65mg l培養基10 ~ 5細胞；抗菌實驗表明，所獲得的重組人乳鐵蛋白具有抑制大腸桿菌生長的作用，而且比人乳鐵蛋白標準品作用更強。
  20. By changing pichia pastoris ' s metabolic path rationally, a constitutive recombinant p. pastoris sibas 5071 with riched sam synthetase was obtained on the basis of research of sulfide ' s metabolism in yeast in our laboratory

    本實驗室在對酵母細胞中含硫物質代謝研究的基礎上，通過合理改造pichiapastoris的代謝通路，獲得了一株組成型表達sam合成酶2 （ sams2 ）的重組p . pastoris菌株sibas5071 。
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