終止體 的英文怎麼說
中文拼音 [zhōngzhǐtǐ]
終止體
英文
cessative aspect-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Seller may also at its election terminate this agreement upon written notice to member in the event member ( i ) fails to observe or perform any other provisions of this agreement to be observed or performed by member and such failure continues for a period of thirty ( 30 ) days after written notice from seller, or ( ii ) becomes insolvent or unable to pay its debts as they mature, or files a voluntary petition or suffers any involuntary petition to be filed against it under any provision of any bankruptcy or insolvency statute, or makes an assignment for the benefit of creditors, or applies for or consents to the appointment of a receiver or custodian for its assets, or any attachment or garnishment is initiated or filed against its property, or ( iii ) dissolves, liquidates, consolidates ( other than a consolidation in which member is the surviving entity ), or ceases to conduct operations
有下列事件賣方可以選擇終止協議（ 1 ）賣方發出書面通知30天後，應該遵守或者履行的成員不能遵守或者履行協議的任何有關規定； （ 2 ）到期破產或者無力償還債務，或者在有關破產或者破產條例規定下自願申請或者遭受強制立案，或者作一個債主賠償費的分配，或者申請或者允許他的資產接受者或者保密人員配置，任何附件或者壓扣件創議或者財產立案； （ 3 ）解散，破產，合併（在合併中成員是存在的獨體） ，或者終止商品營運。A quantitative analysis of this idea led to the prediction that a star remaining about three times the mass of the sun at the end of its evolution ( usually as a neutron star ), will almost inevitably shrink to the critical size needed to undergo a gravitational collapse
該想法的定量分析導致預言一顆恆星在終止它演化時候保持大約三倍太陽的質量（通常看做一顆中子星） ，將會幾乎不可避免收縮到臨界體積必須遭受引力坍塌。Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。The halt study began in 2001 and stopped the hormone therapy arm of the trial after the women ' s health initiatie found in 2002 that estrogen plus progestin increased risk for breast cancer, heart attacks, strokes and blood clots
Halt研究開始於2001年，在2002年婦女健康精神運動發現雌激素加上黃體激素會增加乳腺癌、心臟病、中風和血栓的危險時被迫終止。7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp
7 、首次以fam熒光素標記探針5 』端作為發光基團，以tarma標記探針3 』端為淬滅基團，以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a ）為檢狽目標，設計、篩選出特異性引物和探針，優化實驗參數，建立了轉基因植物通用性熒光pcr定性檢測方法體系。Translation termination and ribosome recycling
翻譯終止和核糖體循環Abstract : solid propellant rocket motors are preferred for most ballistic missiles because they need simple maintenance and can be launched quickly. but the conventional thrust termination devices limit the depletion of every stage ' s grain and increase some extra - weight. an improved method for designing a multi - stage solid rocket based depleted shutdown was provided. in order to solve the problem of lack of thrust termination devices, a device to adjust the burnout angle will match the final burnout velocity and satisfy the desired range. the method can also limit the detection from anti - ballistic missile system
文摘:由於維護簡單和發射快速，彈道導彈多用固體火箭發動機，但繁雜的推力終止裝置使各級裝藥不能耗盡並讓結構增重.提出了一種對基於耗盡關機多級固體火箭概念設計的改進方法，此方法滿足導彈系統主要的戰技要求.為解決無推力終止裝置的末速不準問題，可在末級發動機採用姿態調整裝置，對射角進行調整，配合末速以滿足射程要求.本方法還可抑制敵方反導探測The station will then increment the soft error counter for abort delimiter transmitted, and send a soft error report within two seconds
工作站將增加發送的異常終止分隔符軟體錯誤統計，並且在2秒內發送軟體錯誤報告。Pcr method was used to identify candidate ms 188. sequence analysis indicated that a point mutation occurred in the second exon of the atmyb103 gene in male sterile mutant with caa ( gln ) replaced by a stop codon taa
利用pcr的方法從突變體中擴增atmyb103基因並進行序列分析，結果表明突變體中atmyb103基因第二個外顯子上發生了點突變，由原來編碼谷氨酰胺的caa密碼子突變為終止密碼子taa 。Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions
細胞色素b基因序列變異分析表明： 1 ）雁形目鳥類細胞色素b基因全序列長度一致，無插入和缺失：對照雞線粒體密碼子系統全序列能全部翻譯成氨基酸序列，無無義突變，全序列內部無終止密碼子； 2 ）序列比對后1143加，含416個核著酸變異位點， 306個簡約信息位點，其中處於密碼子第三位的變異最大，第一位和第二位堿基的變異相對較小。Financial - components approach focuses on control and differentiates derecognition of transferred asset from recognition of new financial instruments arising from securitization. it is preferable to the traditional mode, but the inconsistencies and inevitable quantities identification also exists in the approach. comparing to this, continuing involvement approach which focuses on " no continuing involvement " and allows the transfer accounted for as part - sale will delimitate that limitations
美國現行的金融合成分析法將「控制權轉移」作為銷售確認的判斷標準，並且將轉讓資產的終止確認和轉讓所產生的新的金融工具的確認區分開來，克服了傳統會計確認模式的缺陷，但是在具體運用「控制」概念時缺乏內在的一致性，且無法避免「相對數量」的判斷。An analysis of the group s turnover and other revenue is as follows : 2005 2004 hk 000 hk 000 provision of it services 50, 217 15, 342 provision of supply chain solutions 31, 601 10, 892 sales of packaged software products and related services 872 2, 422 system integration - 8, 762 others - 5, 822 - - turnover 82, 690 43, 240 interest income 12 - - - total revenue 82, 702 43, 240 3. profit from operations 2005 2004 hk 000 hk 000 profit attributable to coninuing operations 1, 985 1, 982 discontinued operations 9, 824 9, 628 - - 11, 809 11, 610 4. earning per share the calculation of basic earnings per share for the year is based on the following data : 2005 2004 hk 000 hk 000 earnings profit for the year used in the calculation of basic earnings per share 12, 035 11, 841 shares restated weighted average number of shares in issue for the purpose of calculation of basic earnings per share 421, 208, 703 291, 764, 706 the company completed a rights issue exercise in may 2005 on the basis of one rights share for every one ordinary share held at an issue price of hk 0. 02 per rights share. accordingly, the comparative figures was re - calculated based on the adjusted weighted average number of 291, 764, 706 shares to reflect the effect of the rights issue to the loss per share for the year ended 31st december 2004. 5. final dividend the board does not recommend the payment of a final dividend for the year ended 31st december 2005 2004 : nil
本集團之營業額及其他收益分析如下：二零零五年二零零四年千港元千港元提供資訊科技服務50 ， 217 15 ， 342提供供應鏈解決方案31 ， 601 10 ， 892出售套裝軟體產品及相關服務872 2 ， 422系統集成- 8 ， 762其他- 5 ， 822 -營業額82 ， 690 43 ， 240利息收入12 -總收益82 ， 702 43 ， 240 3 .營業盈利虧損二零零五年二零零四年千港元千港元業務應占盈利虧損持續經營業務1 ， 985 1 ， 982終止經營業務9 ， 824 9 ， 628 - 11 ， 809 11 ， 610 4 .每股盈利虧損年內每股基本盈利虧損乃按以下數據計算：二零零五年二零零四年千港元千港元盈利虧損用於計算每股基本盈利虧損之年度溢利虧損12 ， 035 11 ， 841經重列股份用於計算每股基本盈利虧損之已發行股份加權平均數421 ， 208 ， 703 291 ， 764 ， 706本公司於二零零五年五月完成一項供股，基準為每持有一股普通股獲發一股供股股份，每股供股股份之發行價為0 . 02港元。The amplification system was optimized so that the pcr with different primers can be carried out under the same condition. the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques. sequences were analyzed and compared base on sequencing analysis3. 4 and seq / ede software
方法根據mtdna控制區及其周圍區域的序列，設計多對引物，探索優化擴增體系，使擴增條件能夠同時滿足多對引物的需要，用sanger末端終止法及熒光標記技術對樣本進行dna測序， sequencinganalysis3 . 4和seq ede軟體進行序列分析和比對。A high efficiency dual - band rf power amplifier has an output and / or input of a high frequency transistor well terminated at the second harmonic frequency for dual - band operation
本發明乃一種高效率雙頻射頻功率放大器，提供高頻電晶體的輸出端與?或輸入端于雙頻操作中有良好的二階諧波終止。Andy bodoh, the outgoing president of shield of roses, says that the groupwas begun as a way to personally engage individual men and women. " [ shield ] focuses not so much on ending legal abortion as helping men and women in need, " bodoh told lifesitenews. com
玫瑰盾牌安迪布鐸這位出色的的主席，告訴生命新聞社說，團體中成員都是個別地歸屬這個團體， 」把焦點集中在終止墮胎並協助需要的人」 。With the cases which are retlising to accept admirlistrative punishment, refusing to accept administratix / e coercive measures, retlising to accei ) t the resolution, issued by admin1strative organs, for changing, terminat1ng or disso1vlng some certiticate, retlising t ( ] accept the resolution, issued by administrative organs, for confirming the ownership or utilize ol natural resources. considering the fact that administrative organs infring rlghts of the aut ( ) nomous management, considering the tac1 the thrm contract is changed, abolished ( ) r interfered by administrative organs. considering adminlstrativc organs illegall } levy propertives, apportioned charges, collect capitais, or demand perform1ng other duties, considering administrative orga11s, according to the lau ", shouid issue some certificates or sanction, register some other things which have n ' t be ( : n done, applying administrative organs for performing some legal duties which have n ' t been pefformed, applying adrninistrative organs for issuing consolations, social insurances or the lowest funds of life which have n ' t been issued and considering the concrete administrative conduct of administrative organs infringes other ll legitimate rights and interests
本文運有我國有關法學理論，並結合《行政復議法》的相關規定，對行政復議范圍進行了分析和研究，其中第二章對具體行政行為的復議范圍進行了分析，包括：不服行政處罰決定的行政案件：不服行政強制措施的行政案件；不服行政機關變更、終止、撤銷有火證書的決定的案件；不服行政機關確認自然資源的所有權或者使用權的決定的案件；認為行政機關侵犯合法的經營自主權的案件；認為行政機關變更、廢止或者干涉簽訂農業承包合同，侵犯其合法權益的案件；認為行政機關違法徵收財物、攤派費用、強行集資或者違法要求履行其他義務的案件；認為符合法定條件，申請行政機關頒發有關證書或者審批、登記有關事項但行政機關沒有依法辦理的案件；申請行政機關履行某些法定職責，行政機關沒行依法履行的案件；申請行政機關依法發放撫恤金、社會保險金或者最低生活保障費，行政機關沒有依法發放的案件；認為行政機關的其他具體行政行為侵犯其合法權益的案件等。The high - enzyme activity has 2 base changes, resulting in long amino acid sequence with native amylase. this inducing method resolved the problem of non - effective induction as in base analogue induction. and the method we used provide a new measure for this kind of work
高酶活編碼區位點突變導致c -端序列變化和終止子的后移本誘變方法克服了用堿基類似物在體內誘變由於核酸復制酶等的校正作用而造成誘變無效的難題，為基因的誘變找到了一條新途經。If no quotation for the portion whose recognition has not been stopped is available in the active market and if there is no actual transaction price relating to it, it shall be determined based on the residual amount deducting the consideration of the portion whose recognition has been stopped from the entire fair value of the transferred financial asset
（二）未終止確認部分在活躍市場上沒有報價，且最近市場上也沒有與其有關的實際交易價格的，應當按照所轉移金融資產整體的公允價值扣除終止確認部分的對價后的余額確定。The main types of college teachers ’ appointment contract include : the college teachers ’ of the fixed deadline, the college teachers ’ appointment contract of the deadline to complete the certain work, and the college teachers ’ appointment contract of the special attendance, and also we can get another two types of college teachers ’ appointment contract, which are individual college teachers ’ appointment contract and collective college teachers ’ appointment contract, according to the number of people in one party ; they must be work out by the strict written form, and must pass through the offer and the acceptance step, and don ’ t acknowledge the legal effect of the factual appointment contract ; after the appointment contract becomes effective, the higher college as the appointed party and the teacher as engaged party should completely fulfill the right and obligation provisions in the contract under the instructions of the three principles : fulfill personally, comprehensively, cooperatively. in which, the teachers ’ rights and obligations include : enjoy and undertake the rights and obligations as the specialist engaged in education, teaching and the scientific research ; may change or terminate the contract on the basis of the bilateral consultation, but dismissal and resignation must conform to the agreement or the legal matter ; in the liabilities for breach the contract, be headed by the practical fulfillment, including other two remedial way which are damage compensation and penalty ; in the dispute solution, must establish the perfect concrete mediation system, the arbitration and the civil lawsuit system
高校教師聘任合同主要類型為固定期限的高校教師聘任合同、以完成一定工作為期限的高校教師聘任合同和特殊照顧的高校教師聘任合同，也可依據合同當事人一方人數的多寡不同，也可將個人高校教師聘任合同和集體高校教師聘任合同;其應當以嚴格的書面形式訂立，須經過要約、承諾步驟，且不應當承認事實聘任合同的法律效力;聘任合同生效后，作為聘任方的高等學校和受聘方的教師應當在親自履行、全面履行和協作履行三大原則的指導下完全履行合同中所約定的權利義務條款，其中教師的權利義務內容包括作為一般公民和作為從事教育教學及其科研活動的專業人員所應享有或者承擔的權利義務;經過雙方的協商可以變更或者終止合同，但解聘和辭聘必須符合約定或者法定的事由;在違約責任形式上，應當確立以實際履行為首，包括損害賠償、違約金等三種補救方式;在爭議解決方式上應當建立健全具體的調解制度、仲裁和民事訴訟制度。分享友人