組分粒子質量 的英文怎麼說
中文拼音 [zǔfēnlìzizhíliáng]
組分粒子質量
英文
component particle mass- 組 : Ⅰ名詞1 (由不多的人員組成的單位) group 2 (姓氏) a surname Ⅱ動詞(組織) organize; form Ⅲ量詞(...
- 分 : 分Ⅰ名詞1. (成分) component 2. (職責和權利的限度) what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] (料想) judge
- 粒 : Ⅰ名 (小圓珠形或小碎塊形物) small particles; grain; granule; pellet Ⅱ量詞(用於粒狀物)
- 子 : 子Ⅰ名詞1 (兒子) son 2 (人的通稱) person 3 (古代特指有學問的男人) ancient title of respect f...
- 質 : Ⅰ名詞1 (性質; 本質) nature; character; essence 2 (質量) quality 3 (物質) matter; substance;...
- 量 : 量動1. (度量) measure 2. (估量) estimate; size up
- 粒子 : grain; granule
- 質量 : 1 [物理學] mass 2 (產品或工作的優劣程度) quality 3 economy (離子源的); 質量標準 quality level...
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Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板,用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增,得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上,篩選反向克隆,然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游,構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上,通過花粉管通道法轉化河套蜜瓜,共獲76顆瓜,並進行了硬度和含糖量的分析。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。Specifically, to a test particle, its mass defect is am = m _ ( 0 ) - m, where mo and m are the mass of the particle when it is in the infinity and in the grav - itational field, respectively. to a spherical shell ( or a solid sphere ), its mass defect is am = m _ ( 0 ) - m, where mo is the total mass of the matter scattering in the infinity and m is the mass of the gravitational spherical shell ( or the gravitational sphere ) combined by the matter scattering in the infinity
具體來說,對于試驗粒子,當它由無窮遠處運動到引力場中某點時,其質量由m _ 0變為m ,發生的質量虧損為m = m _ 0 - m ;對于球殼或固體球,當組成球殼或固體球的這些物質分散在無窮遠處時,總質量為m _ 0 ,當這些分散在無窮遠處的物質結合成球殼或固體球時,其質量變為m ,發生的質量虧損為m = m _ 0 - m 。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示:重組質粒轉染的細胞質中有棕褐色顆粒,而空載體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清,成蟲蟲體可溶性抗原免疫兔血清, ts87基因原核表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛蟲單抗特異識別。After completing the mctf using db2 wavelet, it is well integrated with discrete wavelet transform ( dwt ) and embedded zero tree wavelet. it uses atom structure to organize the coded bit - stream to achieve the brilliant combination of three scalabilities : temporal, spatial and psnr scalabilities. and the software platform is based on vc + + 6. 0
在基於db2小波的運動補償時域濾波方法實現之後,本文將之和離散小波變換( dwt ) 、嵌入式零樹編碼進行有機結合,並採用基於基本原子粒的數據流組織結構將分層后的數據流有效地組織起來,實現了具有時間、空間、質量三方面的完整可伸縮性的編解碼系統,系統的軟體平臺基於vc + + 6 . 0實現。By means of the constiuent gluon model in which gluon has dynamical mass and one spin, the potential of two - gluon glueball systems can be obtained. from the hamiltonian of two - gluon glueball systems, we can calculate the spectrum of two - gluon glueballs
在分析膠球譜時,採用組分膠子模型,把膠子看作有質量的自旋為1的粒子,得到相應的相互作用勢,從而用得到的哈密頓來求解膠球譜。When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum
莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎,同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達,所構建的重組質粒ptga ,其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 , 5 、 4 、 2 . 3倍,且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中,搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率,分別為0 . 448 %和22 . 4 % 。To investigate the space / time distributions and occurrence and evolution of such events, the diffusion pattern over deserts, turbulent transfer features in sandstorm weather, the particle size distribution, mass concentration and its distribution, optic properties, chemical composition and physical factors responsible for the initiation of raising sands, we made integrative observation and sounding of sandstorms deep in the large - scale desert area, including tengri, badanjilin and maowusu, with the items consisting of micrometeorological measurement, 3d wind observation, data from kb - 120 and anderson samplers of mass concentrations of sands with their spectrum, the distribution of aerodynamic particle sizes from the aps3310a, retrieval of aerosols " optic depth from sunphotometer data, assay of the chemical composition by means of neutron activiation analysis ( naa ) and integrated study of all related factors for causing sandstorm to occur, based on the observations of all kinds
利用所取資料,系統分析了不同強度沙塵天氣條件下沙塵氣溶膠質量濃度和質量濃度譜、粒子譜分佈、光學厚度、化學組分等特徵;綜合分析了影響沙塵起動的諸物理因子在沙塵起動中的作用;用沙塵輸送模式對一次沙塵暴天氣造成的泥雨過程的形成機制進行了模擬。歷史氣象資料統計分析表明,沙塵暴有其高發期( 4 、 5月)和高發時段( 14 - 20時) , 14時到20時之間發生的沙塵暴約占沙塵暴總次數的66 。揚沙和沙塵暴天氣條件下,湍流動量通量和湍流感熱通量都是重要的湍流交換,沙塵暴發生前近地層的超絕熱不穩定對沙塵暴天氣有加強作用。Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母, g418篩選出多拷貝插入的單克隆,甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白,培養液上清經sds - page電泳分析,在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ,能與羊抗hcmv多克隆抗體發生發應的條帶。In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定,該基因為雞il - 2基因,其序列與sundick等報道的完全一致。在此基礎上,我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ ( tm ) ht中,構建重組表達質粒並進行確證性序列測定,重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ ( tm ) ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養, sds - page和westernblot分析顯示,表達的雞il - 2融合蛋白分子量約為18kda ,表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。Sds - page analysis suggested that the bacteria containing the recombinant plasmid pet - 32a ( + ) - igf - i produced the fusion protein of 30kda as it was induced by iptg. consisting 10 % of the total bacterial proteins, and the pet - 30a ( + ) - igf - ii produced the fusion protein of 14kda, which consisting 35 % of total bacterial proteins. 5
Sds - page分析表明,重組質粒pet - 32a ( + ) - igf -在iptg誘導下表達分子量約30kda的融合蛋白,但其表達量不高,約為菌體總蛋白的10左右;重組質粒pet - 30a ( + ) - igf -在iptg誘導下表達分子量約14kda的重組蛋白,融合蛋白表達量約占菌體蛋白總量的35 。The recombinant plasmid was translated into e. coli dh5 and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 22ku protein which was equal to chicken ifn - y protein in molecular weight was expressed in e. coli dh5
將重組質粒轉化大腸桿菌dh5 ,於37誘導培養8h , sds - page凝膠電泳表明該基因在大腸桿菌中獲得了高水平表達,表達的雞ifn -融合蛋白分子量約為22ku 。This part emphasizes the synthesis of nanoarrays, aiming at controlling the size and distance of nanocrystallites using calixarene derivatives by altering the size, length and chemical structure of the organic molecules ; 2. this part emphasizes in situ synthesis strategy for fabrication of polymer network of zns based nanopowder, aiming at size controls, coating and preventing agglomeration following " one - pot " synthesis ; this method fits to low cost, large scale production ; 3. according to development in zno nanomaterials, we first report on the synthesis, characterization of amorphous zno, aiming at describing the principles and approaches of synthesis techniques, optical properties, spatial structure and doped effect ; the amorphous zno displays cage - like structure, showing a strong ultraviolet emission while the visible emission is nearly fully quenched, a potential uv - emission material ; 4
本論文以量子結構自組裝為出發點,提出利用杯芳烴及其衍生物的化學受限反應實現尺寸可調半導體納米粒子自組裝;提出有機聚合網路原位組裝zns基納米熒光粉方法,把熒光粉的納米化、包敷、防團聚在「一鍋」反應中完成,適于低成本,批量生產;根據當前zno的研究情況,我們首次合成了非晶zno ,研究了它的光學性質,確定了它的結構,並對其摻雜進行了初步的研究,非晶zno表現出強的深紫外發光特性,而可見發射非常弱,是一種有巨大潛在應用價值的深紫外發光材料;利用非晶zno的亞穩特性,對晶化過程中非晶zno納米晶zno三維受限量子結構特性,界面特性進行了深入的研究;利用固相熱分解一般受擴散控制特性,實現了尺寸可控的zno三維量子結構的自組裝;利用非晶zno的高度分散性,容易均勻成膜特性,實現了非晶籽晶誘導低溫液相外延自組裝生長高取向zno晶體薄膜。In the present research, scanning electron microscope ( sem ), laser raman spectroscopy ( lrs ), x - ray photoelectron spectroscopy ( xrs ), x - ray diffraction ( xrd ) and electron probe micro analysis ( epma ) were utilized to investigate the difference in micro - structure and elements distribution between domestic and foreign pdcs. combined with analysis on current manufacturing process, the mechanism for the difference was discussed. scanning electron microscope ( sem ), laser granularity analysis, atom emission spectroscopy ( aes ) and plasma emission spectroscopy ( icpaes ) are also utilized to investigate the grain shape and impurities of key material - diamond power
本課題採用掃描電鏡、拉曼光譜、光電子能譜、 x -射線衍射分析、電子探針等方法分析了國內外聚晶金剛石-硬質合金復合片在微觀組織結構、元素成分分佈方面的差異,結合對現有燒結工藝的分析,研討了造成這些差異的機理;採用掃描電子顯微鏡、激光粒度分析、原子發射光譜、等離子發射光譜等方法對關鍵原材料-金剛石微粉的晶形、雜質含量進行了比較分析測試。The results were found that the specific 670bp dna bases of cgh was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 50 kda which is consisted of a 23. 6 kda protein deduced from the cgh gene sequence and gst ( 26 kda )
Rt - pcr分析確證重組質粒在大腸桿菌中能正確轉錄; sds - page分析證明誘導重組質粒表達了融合蛋白,分子量約為50kd ,與預期的26kd的gst帶和23 . 6kd的草魚生長激素基因編碼蛋白質構成的融合蛋白大小一致。The pcr products were purified and linked with pmd 18 - t vector respectively. the positive recombinants were selected and digested with double restriction enzymes respectively. goat fsh - a and p subunit genes which had sticky ends were purified and linked respectively with pf which also had the corresbonding sticky ends and were purified
將所得的山羊fsh - 、亞基基因分別用加相應限制性內切酶酶切位點的引物大量擴增,回收后與pmd18 - t連接,篩選出陽性重組子,大量雙酶切回收獲得帶粘性末端的山羊fsh - 、亞基基因,將它們分別與同兩個酶大量雙酶切回收獲得的帶粘性末端的pf質粒進行連接,篩選陽性重組子。With calculating one can observe the n * ( 1440 ) resonance peak obviously in the invariant mass spectrum of n system, and in daliz plots the event number distribution is density, there are no such phenomenons in other composed systems. all of these things we have got will be helpful to the imminent experimental detection
通過計算我們可以得到末態各個粒子各種可能的信息,在n的不變質量譜上有明顯的共振峰出現,在達里茲圖的相應位置處事件分佈密集,而在其它組態中卻沒有這種現象,這都說明了n ~ * ( 1440 )的產生。Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme
作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切,電泳回收目的片段,將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中,轉化大腸桿菌tgi感受態細胞,得到的轉化子經pcr鑒定和酶切分析,篩選出符合閱讀框的重組子,構建成重組表達質粒pet - hn ,並在大腸桿菌bl _ ( 21 ) ( de _ 3 )宿主菌中成功地表達了含目的蛋白的融合蛋白,融合蛋白的分子量約66kd ,加入iptg誘導8h后,蛋白表達幾乎達到最高水平。The numbers and amplitudes of both transimtted and reflected solitons from an incident soliton are given analytically for this case. if the interface of two kinds of dust grains is continuous, neglecting the reflection, the nonlinear dust - acoustic wave can be described by a kdv - type equation in the lowest order. the amplitudes, propagating velocities of these quasi - solitons for this case are also given analytically
無論是分界面不連續變化還是連續變化,對于小的、但有限振幅的長波振動,電勢孤子從質量小的塵埃等離子體穿過分界面進入質量大的塵埃等離子體,電勢孤子的振幅將增大,速度將變快,反之,電勢孤子從由質量大的塵埃微粒組成的塵埃等離子體穿過分界面進入由質量小的塵埃微粒組成的塵埃等離子體,電勢孤子的振幅將減小,速度將減緩。分享友人