結核菌胺 的英文怎麼說
中文拼音 [jiēhéjūnàn]
結核菌胺
英文
tuberculosamine-
Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3. the recombinant plasmid was transformed to e. coli bl21 and the gst / mpem fusion protein was induced to express with iptg. the fusion protein was purified by affinity chromatography
方法: pcr擴增小鼠pemcdna編碼序列,將它克隆到含有gst的原核表達質粒載體pgex - 4t - 3上,轉化大腸桿菌bl21 ,誘導表達gst mpem融合蛋白,通過親和層析,獲得初步純化的產物,以羥胺切割驗證其一級結構。
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