In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv
理化學研究表明,該病毒為rna病毒,對氯仿、乙醚敏感;胰酶試驗中,
經37 、 1小時處理的病毒,仍然能夠在貓源細胞fcwf細胞上生長,並且毒力基本保持不變;耐酸性試驗中,病毒分別在ph5 . 0和ph3 . 0
經37作用2小時,毒力僅下降一個滴度;耐熱性試驗中,該病毒在恆定溫度50 ,設定不同時間,從5分鐘到150分鐘,毒力均有不同程度下降,其中, 50作用30分鐘,病毒平均滴度下降2個單位; 50 , 60分鐘, cpe消失;恆定時間1小時,設定不同溫度( 50 - 60 - 70 - 80 ) ,病毒在細胞上完全喪失增殖能力, cpe消失。生物學試驗,利用實驗室現有條件,選擇不同的細胞系對該病毒進行培養,發現該病毒對貓源細胞fcwf最敏感; mdck細胞次之; f81細胞
經多次傳代,亦可出現cpe ;而vero細胞則不敏感。血凝試驗表明,該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
Unfortunately, there was still light pollution although technology was improved many times. to ensure environment protection, wanbao company decided to remove all the old product lines and reinstall the product lines of oxide electrolysis. the total set is 30, and equipped with two 25kg vacuum induce stoves, the yield is 1400 mt per year. the main production are low - zinc low - magnesium lanthanum metal, low - zinc low - magnesium mischmetall, neodymium metal, didymium metal, yttrium metal, dysprosium metal etc
多年來,萬寶公司一直從事氯化物電解生產,雖
經多次環保治理,仍有一定污染,為確保綠色環保生產,公司決定將原有生產線拆除,全部改裝成氧化物電解生產,總臺數為30臺,年產量為1400噸,同時配備兩臺25kg真空感應爐。主要生產低鋅低鎂金屬鑭低鋅低鎂混合稀土金屬金屬釹鐠釹金屬
Lew has been on the verge of asking fay to marry him many times, but he ' s too shy to take the plunge
盧已
經多次想要向費依求婚了,但是由於過于害羞,話到舌尖卻沒有開口。
And in 1990, after years of research, a whole new family of rhone - style wines was introduced under the vin du mistral label and included syrah, viognier, grenache ros, and a delicious red wine blend called le mistral
經多年研究之後,酒園於1990年推出了密斯崔爾酒屬全新的羅納谷式葡萄酒,包括席拉、維歐尼耶、歌海娜以及密斯崔爾紅酒。
The physical conditions of each country, as well as the numbers and kinds of its inhabitants, have undergone many mutations.
每一地域內的物理條件和土著生物的種類與數目,都是曾
經多次變動。
After much discussion the negotiators hammered out a compromise settlement.
經多次協商,談判者達成一項折衷的解決辦法。
Multiple endorsements on to order cheques or other orders or instruments will not be accepted by the bank unless prior arrangements have been made
除非已有事先的安排,否則本行不會接納
經多次背書之記名支票或其他付款指示或票據。
I have superintended cooking picnics.
我曾
經多次主持過這種生火的野餐。
Through reiterative study over years by the author, a new principle to express the relation between aggregate particles size and the surface area was proposed, that is the specific granularity, which could solve the problem of lack of correlation of fineness modulus to the specific surface area of the sand
摘要
經多年反復研究,發現了集料粒度與表面積關系法則,提出了以「比粒度」表述砂石粒度的新方法,解決了以「細度模數」表述砂子細度的不足之處,即解決了細度模數與不同細度砂子的比表面積沒有相關性的問題。
Abstract : architecture expression is produced from the procedure of several times “ translation ”. according to the theory of semeiology, combing the viewpoints connected, this paper discusses of the reliability “ translation ” and the action and the meanings of content signified in every “ translation ”
文摘:建築表意是一個歷
經多次「轉譯」的過程.根據符號學原理,結合有關觀點,論述這種「轉譯」的可靠性和每一次轉譯中被意指內容的作用和意義
Also decoloring rate and photocatalytic degradation of methylene blue were high for tio2 / sepiolite and tio2 / zeolite, and so much as sample reusing several times ; 4
發現tio _ 2天然礦物復合材料,對有機染料的降解,
經多次重復使用仍然具有很好的光催化降解性能; 4
Fluorescein angiography showed leaking telangiectasia, macroaneurysm in the left eye but no abnormal leakage in the right eye
左眼
經多次局部雷射光凝固治療后,最佳矯正視力可達零點陸。
Dorts and trtc taipei rapid transit company have been working to find effective measures to solve these problems and reduce complaints from residents. the following measures have been carried out
本局與捷運公司經多方努力,找出各種方法克服以減低因捷運帶來之公害所引起之民怨。