Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚
繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，
利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
He used aphid fecundity data to elucidate the theoretical effects of antibiosis on insect population dynamics.
他
利用蚜蟲
繁殖力資料說明了抗生作用對蟲口動態的理論效應。
This rabbit had no natural enemies in the antipodes, so that it multiplied with that promiscuous abandon characteristic of rabbits
這種兔子在澳大
利亞及紐西蘭沒有天敵，因此便以兔子所特有的雜亂交配迅猛
繁殖起來。
Arranged successively in ascending powers of hierarchical order, that of gardener, groundsman, cultivator, breeder, and at the zenith of his career, resident magistrate or justice of the peace with a family crest and coat of arms and appropriate classical motto semper paratus, duly recorded in the court directory bloom, leopold p., m. p., p. c., k. p., l. l. d. honoris cause, bloomville, dundrum and mentioned in court and fashionable intelligence mr and mrs leopold bloom have left kingstown for england
按照越往上權
利越大的等級制度順序，他曾經是園丁莊稼人耕作者牲畜
繁殖家仕途的高峰是地方長官或治安推事。他擁有家徽和盾形紋章以及與之相稱的拉丁文家訓時刻準備著，他的名字正式記載于宮廷人名錄252中布盧姆，
利奧波德保，下院議員，樞密顧問官，聖巴特里克勛級爵士253 ，名譽法學博士。
Deng wh, gao w, zhao j. 2004. breeding biology of grey - faced buzzard in northeastern china. journal of raptor research 38 : 263 - 269
王海濤、高瑋、萬冬梅、劉多、鄧文洪. 2003 .
利用天然樹洞
繁殖的五種鳥的巢位特徵及
繁殖成功率.生態學報， 23 ： 1377 1385
I'm a busy lawyer, miss molineaux, with a heavy case load.
我是個工作
繁忙的律師，莫
利諾小姐，手裡要處理的案件多得很。
Dear god, wrigley, how many cockamamie personal possessions do we have to amass -
天哪賴
利我們還有多少
繁瑣的私人收藏要收集啊
Dear god, wrigley, how many cockamamie personal possessions do we have to amass - -
天哪賴
利我們還有多少
繁瑣的私人收藏要收集啊
The immediate effect of this great dispute upon the ideas and methods of people in the prosperous and influential classes throughout the westernized world was very detrimental indeed.
這次大爭辯對于整個西方化世界
繁榮富庶的和有影響的階級人士的思想和方法的直接影響，的確是很不
利的。
When profit diminishes, merchants are very apt to complain that trade decays; though the diminution of profit is the natural effect of its prosperity.
當
利潤減少時，商人們往往都埋怨說，商業衰退了，可是
利潤減少乃是商業
繁盛的自然結果。
The implantation of a property and prosper, must depend on a well - known brand and production, big enterprise, group leader business enterprise or business enterprise group to develop, the author bring up of clear and definite a target ( building the medicine flattop, making wudang a medicine harbor ), push forward two development way of million - project ( plant a million mu chinese herbal medicine, arouse 100 ten thousand farmers amass a fortune with the middle - class family ), integrating three greatest resources ( chinese herbal medicine, capital and human resource ), classification raising the hormone medicine, inside the patent medicine and bio - medicine, vet ' s medicine with medical treatment apparatus and drugs packing a four group leader business enterpriseses, and it is fit for the practice ; the author bring forward that the framework of chinese herbal medicine by company add households, going by business enterprise mechanism to circulate, and accord with the busi ness enterprise and the farmer benefits, it carry on actually
一個產業的培植與
繁榮，必須依靠龍頭企業或企業集團的帶動，靠一批骨幹企業一批知名品牌、產品作支撐，才能促進該產業可持續發展。作者提出的明確一個目標（打造醫藥航母，建設武當藥港） ，推進兩個百萬工程（種植100萬畝中藥材，帶動100萬農民致富與小康） ，整合三大資源（中藥材、資本與人力資源） ，分類培植激素類藥物、中成藥與生物醫藥類、獸醫藥類和醫療器械與藥品包裝類四個龍頭企業的發展思路，切合該地區實際；作者提出的公司加基地加農戶加依託單位的中藥材種植模式，按企業機制運行，符合企業和農民
利益，切實可行。
Many trees can be propagated from vegetative shoots, thus reproducing their genotypes exactly.
許多樹木能夠
利用營養枝
繁殖，這樣，它們的基因型可以精確地傳衍。
Bloom was pointing out all the stars and the comets in the heavens to chris callinan and the jarvey : the great bear and hercules and the dragon and the whole jingbang lot
布盧姆把天上所有的星星和慧星都指給克里斯卡
利南和車把式看：什麼大熊座啦，武仙座啦，天座啦，和其他
繁星。
The coastal lagoons of ojo de liebre and san ignacio are important reproduction and wintering sites for the grey whale, harbour seal, california sea lion, northern elephant - seal and blue whale
地處奧霍德列夫雷和聖伊格納西奧的沿海環礁湖，是灰鯨、港灣海豹、加
利福尼亞海獅、北方海象以及藍鯨的重要
繁殖地及越冬地。
In addition, many would agree that as a society we bear the ethical obligation to protect the habitability of the planet, and to act as responsible stewards of its bio logical riches for the present and future welfare of the human species
另外，許多人都同意的是，作為一個社會我們承擔著道德上的義務來保護星球的可居住性，並為人類目前和未來的福
利充當生物
繁榮的有責任的義務員。
Directed on the present situation and existing problems in boosting beef cattle industry based on the yellow cattle improvement in china, the review indicated that several relationships, such as integration of individual households and large scale cattle farms, integration of yellow cattle improvement, crossbreeding, breed conservation and utilization, integration of high grain diet intensified fattening and low grain diet economic fattening, should be handled properly ; and the requirements of profitable cattle industry for increasing reproductive survive rate, using hybrid vigor and improving the weight gain, the main factors affecting economic return of large scale cattle farms and the technology level improvement in cattle industry were duiscussed as well
本文就近來我國以黃牛改良為特點的肉牛業蓬勃發展現狀與存在問題，指出應處理好養牛經營形式千家萬戶與規模牛場相結合、黃牛肉用與肉役兼用相結合、黃牛改良選育與雜交、保種及
利用相結合、高精料強度肥育與低精料適度育肥相結合等幾個問題；並就提高養牛經濟效益對提高
繁殖成活率、充分
利用雜種優勢、提高日增重的要求和影響規模牛場經濟效益的主要因素及如何提高養牛科技水平等問題提出探討。
In her position, monica travels frequently to new iberia which has had booming economic in recent years thanks to outsourcing for large international corporations for software development
出於工作原因，近年來莫妮卡頻
繁地往來于新伊比
利亞地區。大型國際公司為了軟體發展來此采購，使此地經濟迅速發展。
The complex is one of the largest and busiest interchanges in the state of illinois.
它是伊
利諾斯州最大和最
繁忙的立體樞紐之一。
Reforms usually begin with extra indexation and more frequent adjustment of controlled interest rates.
改革往往始於額外指數化，或者更加頻
繁地調整控制
利率。
Mr bashir ' s survival has also been helped by an economic boom, at least in khartoum
巴希爾政權的倖存也得利於經濟的繁榮，至少是在喀土穆是這樣。
