耐熱酶 的英文怎麼說
中文拼音 [nàirè]
耐熱酶
英文
heat resistance enzyme-
In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv
理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。Milk and milk - based products - detection of thermonuclease produced by coagulase - positive staphylococci
乳和以乳為基料的製品.凝固酶-陽性葡萄球菌的耐熱核酸酶檢測Unlike enzymes cofactors are, in general, stable to heat
和酶不同，輔助因子往往是耐熱的。Hsap heat stable alkaline phosphatase
耐熱性堿性磷酸酶The progress on the research of thermostable enzyme in the food industry
耐熱性酶在食品中的應用研究進展The synthesized single strain oligonucleotides were separated by high performance liquid chromatography. the thermostable dna polymerase was used to catalyze snupe and the reactions of snupe for two y - snp loci were developed
利用耐熱dna聚合酶催化單堿基引物延伸反應，建立2個y - snp的單堿基引物延伸反應。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。The micro particle acidic proteinase was studied respectively in respects of its heat stability and storage stability, when 4 % of it was added in a pre - mixed pig feed
摘要分別從耐熱性及在4 %豬用預混料中的貯存穩定性兩個方面，對微丸型酸性蛋白酶進行了研究。The results shows that the micro particle acidic proteinase is characterized by excellent heat satiability and resistance against acid, choline and high concentration meal ions
結果表明，微丸型酸性蛋白酶具有良好的耐熱性以及在預混料中耐酸、膽堿、高濃度金屬離子等性能。The human placental alkaline phosphatase ( hplap ) ( ec 3. 1. 3. 1 ) would be used more widely in molecular biology and elisa for its enzyme activity and heat resistant was higher than that of e. coli alkaline phosphatase ( eap ) and calf alkaline phospha - tase ( c1ap ). it is promising to construct the gene engineering pichia pastoris to product the hplap in a scale for the resource of hplap is limited
L ）的活性和耐熱性都高於常用的大腸桿菌堿性磷酸酯酶（ eap ）和小牛腸堿性磷酸酯酶（ ciap ） ，因而更適用於分子生物學和酶聯免疫領域，然而其米源受到限制，探討利用畢赤酵母尺屍astorjs構建工程菌進行規模生產鳳有） 』 『闊的前景。Edta and naf could inhibit the enzyme activity intensively, however, it was obviously that no effect of nacl on the phosphatase activity
該酶耐熱，在50時活力達到最高，對nacl不太敏感。但edta和naf可以明顯影響該酶的活性。分享友人