Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄
聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性
片段,將此
片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
Especially in autumn and winter when the air is not so unstable, cumulus clouds may merge and cover a large part of the sky. they become altocumulus
此外,尤其是秋天和冬天時,天氣沒那麼不穩定,積雲會
聚合在一起,一大
片的覆蓋部份的天空,出現了高積雲。
Cymes 1 - 2 in axil of leaves, rather short ; flowers pale green, 7 mm in diam., 5 - merous ; sepals imbricate, inner two larger ; petals suborbicular, 3 mm long ; stamens without filaments ; pistil without style, stigma appressed, minutely 5 - lobed
聚傘花序1 - 2腋生,短小;花淡綠色,直徑約7毫米; 5數;萼
片覆瓦狀排列,內方2
片較大,邊緣常有深色細淺齒;花瓣近圓形,長約3毫米;雄蕊無花絲;雌蕊無花柱,柱頭平貼,微5裂。
So then after that they drifted on to the wreck of daunt s rock, wreck of that illfated norwegian barque - nobody could think of her name for the moment till the jarvey who had really quite a look of henry campbell remembered it, palme, on booterstown strand, that was the talk of the town that year albert william quill wrote a fine piece of original verse of distinctive merit on the topic for the irish times breakers running over her and crowds and crowds on the shore in commotion petrified with horror
那個長得確實像亨利坎貝爾的水手終于想起來了,船名「凡爾默」號,是在布特爾斯湯岸灘觸的礁,成了當年全城人的話題-艾伯特威廉奎爾還以此為題替愛爾蘭時報寫了一首富於獨創性的極出色的佳作。碎浪花沖刷著船身,成群的人們
聚在海岸上,一
片混亂,一個個嚇得呆立在那裡。
In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank
本實驗採用了高保真pfudna
聚合酶,在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性
片段,將此
片段插入克隆載體pgem - 7fz ( + ) ,經測序和序列分析表明,所擴增得到的
片段含有bar基因完整的讀碼框,並且序列與genbank中發表的序列完全一致。
In the daisy family the numerous bracts which subtend the inflorescence are known as an involucre
在菊科植物中,苞
片數目多而
聚生在花序外圍,稱為總苞。
Besides acting as agent of huzhou zhanwang pharmaceutical co., ltd. for a variety of adjuvant, we are also the agent for many imported adjuvant, such as new zealand wyndales lactose ; germany basfs vitamins and direct compression excipients ; france roquettes polyatomic alcohol ; germany sasols polyglycol ; switzerland givandans powdery flavoring essence ; usa cabots gas silica ; italy randi groups tartaric products ; uk crodas tween and span ; japan asahikaseis microcrystalline cellulose ; usa noveons carbopol resin, etc
我公司除代理湖州展望化學藥業有限公司各種輔料,同時代理多家進口輔料,諸如:紐西蘭乳糖系列產品、德國巴斯夫公司維生素系列產品及直接壓
片輔料;法國羅蓋特多元醇系列產品;德國沙索公司
聚乙二醇系列產品;瑞士奇華頓粉末系列香精;美國卡博特公司氣相法二氧化硅;義大利拉第集團天然酒石酸系列產品;英國禾大公司吐溫,司盤系列;日本旭化成公司微晶纖維素系列產品;美國諾譽公司卡伯波樹脂系列等。
Its distinctive colour is a mix of hues and its scales are so calcified that they shimmer like pearls
石灰質積
聚于鱗
片之上,仿如珍珠,因而得名。
Calendered film from flexible pvc
軟
聚氯乙烯壓延薄膜和
片材
Flexible cellular polymeric materials - sponge and expanded cellular rubber products - part 1 : sheeting
高
聚物多孔彈性材料海綿與多孔橡膠製品第1部分:
片材
Flexible cellular polymeric materials - sponge and expanded cellular rubber products - specification - sheeting
柔性多孔
聚合材料.海綿和膨脹的多孔橡膠製品.第1部分:壓
片
Abstract : the formation technologies, the structure and properties of tcs microporous man - made yarn - kind new polyester fibre which was spinned in tcs with some micrometallic - oxide have been studied. the results indicate that the rheological behaviour and spinnability of commixed polyester melt, and the structure and properties of the fibre, are relative to the spinning temperature, melt pressure, microporpus size for spinning, formation conditions, thermo - vascellum temperature, thermo - vascellum position and rolling speed, besides the viscosity of polyester cuts, coacervation particles, adding amount and size of micrometallic - oxide
研究表明, tcs微孔化共混
聚酯熔體在成型加工中的流變行為和可紡性,及其纖維的結構性能除了與原料切
片的特性粘度、凝
聚粒子和共混
聚酯中超細金屬氧化物微粒的添加量、粒徑等因素的影響有關外,還與紡絲溫度、紡絲熔體壓力、噴絲板微孔尺寸、冷卻成形條件、熱管溫度、熱管安裝位置以及卷繞速度等因素的影響密切有關。
Microscopes. connecting dimensions of dismountable condenser and colour filter
顯微鏡.可拆卸之
聚光鏡及濾色
片的接尺寸
We have scanned more than 10 aerogel tiles in the cosmic dust lab in houston and have recently written a computer program that checks the focus on each of the 40, 000 focus movies we have so far
在休斯敦的「宇宙塵埃實驗室」我們已經掃描了超過10個氣凝膠塊,並新近編寫了一個程序用來對迄今為止得到的4萬個
聚焦影
片進行逐一檢查。
Main products include : segment ferrite magnet, sintered permanent ferrite magnet, bonded permanent magnetic powder, alnico granule, ferrite granule, magnetic door gaskets, hard pvc profiles, pvc granule, sintered ndfeb magnet - arc shape, sintered ndfeb magnet, and flexible rubber magnet, which are widely used in aerospace, telecommunication, car, home appliances, toys, and office. over the past few years, we have earned excellent reputation from satisfied customers at home and abroad
公司的主導產品為:永磁鐵氧體磁瓦燒結永磁鐵氧體磁鐵粘結永磁鐵氧體料粉alnico匯
聚片粒料注射成型用各類同性異性尼磁粒料及磁體磁性門封硬pvc型材pvc粒料燒結釹鐵硼磁體及橡塑磁體,廣泛應用於航天航空電訊電聲汽車配件家用電器玩具文教和辦公用品。
Having a light moment at the tea break
嘉賓在中場茶聚片刻