胰蛋白酶 的英文怎麼說
中文拼音 [yídànbái]
胰蛋白酶
英文
trypsase; parenzyme; trypsin-
Protective effect of urinary trysin inhibition on sinusoidal endothelial cell injury during hepatic preservation ane reperfusion
尿胰蛋白酶抑制劑對肝臟保存再灌注中肝竇內皮細胞損傷的保護作用The he was strongly inhibited by sbti and apmsf, and very sensitive to pmsf, lbti, and tlck, while not sensitive to chymostatin, bestatin, leupeptin, tpck, pepstatin, nem and iam. all these results imply that brine shrimp he was most probably a trypsin - type serine protease. the he could be strongly inhibited by edta in a dose - dependent manner, and 50 mmol / l edta exhibited more than 56. 5 % inhibition
對孵化酶純化樣品進行生化性質和酶性質分析發現，鹵蟲孵化酶的最適反應溫度約為40 ，最適ph為8 . 5左右；該孵化酶對p - apmsf 、 sbti極為敏感，對pmsf 、 lbti和tlck也非常敏感，但對chymostatin 、 leupeptin 、 pepstatin 、 bestatin 、 tpck 、 nem和iam不敏感，表明該酶極可能是一種屬于胰蛋白酶類型的絲氨酸蛋白酶。Compared to control, retention times of digesta in whole alimentary tract of immunized animals inc reased by 20 hours ( to use cumlative excretion of 5 % marker as reference ). immunoneutralization of ss significantly augmented activities of digestive enzymes ( proteolytic, trypsin, chymotrypsin, amylase ) in pancreas and the small intestine ( control and immunized animals were 1693. 67unit / g, cp, 2728. 33 unit / g, cp, 3055. 50 unit / g, cp, 12. 9x106 unit / g, cp ; 2. 57x 102unit / g, cp, 1. 20x103unit / g, cp, 1. 12x 103unit / g, cp, 2. 98x 107unit / g, cp ft 2451. 33 unit / g, cp, 2904. 17 unit / g, cp, 4279. 33 unit / g, cp, 20. 61 x 106 unit / g, cp ; 6. 45 x 102unit / g, cp, 2. 53 x 103unit / g, cp, 1 - 83 x 103unit / g, cp, 5. 77 x 107unit / g, cp, respectively, p < 0. 05 or p < 0. 01 )
12ng ml ， 0人su vg ，各指標比較均差異不顯著， p 0刀5人兔疫組動物的食糜消化道滯留時間明顯增加（以指示劑累計排出50為標準，兔疫組較對照組大約增加20小時） ，與此同時， ss免疫中和也提高了胰腺和消化道各種消化酶的比活力（對照組和免疫組胰腺，小腸食糜總蛋白酶，胰蛋白酶， 」糜蛋白酶和澱粉酶比活分別為1693石7unit g ， cp ， 2728Studies on resistant induction of citral to candida albicans in vitro
永川豆豉胰蛋白酶抑制劑的分離純化及其降糖活性研究Fixable conditions and function of trypsin
胰蛋白酶固定化條件及穩定性對比研究The hydrochloric acid in gastric juice is the main cause of the inactivation of pigg
相對而言，胃酸造成的變性要較胰蛋白酶的水解對pigg活性的影響更大。The detecting linear range is 0. 23 - 23. 9ng / ml
9pg ml范圍內測定了抗胰蛋白酶。Methods for quantitative analysis of immobilized trypsin
備用胰蛋白酶的定量分析法Study of the hydrolysis of the degreased soja draft by trypsin
胰蛋白酶水解脫脂豆粕的研究Proteins - trypsin - methods for quantitative analysis
蛋白質.胰蛋白酶.定量分析方法Measurement of trypsin inhibitor activity of soya - bean products
大豆製品中胰蛋白酶抑制劑的抑制活性測定Animal feeding stuffs - determination of trypsin inhibitor activity of soya products
動物飼料.大豆製品胰蛋白酶抑制劑活性的測定Preparation and application of monclonal antibodies against trypsinogen activation peptide
抗胰蛋白酶原激活肽單克隆抗體的制備及應用Here, we demonstrate on - line, real - time tryptic digestion in conjunction with reversed - phase protein separation
這里，我們證明了與反相蛋白分離相結合的在線、實時胰蛋白酶解方法。Proteins were identified with peptide mass fingerprinting using matrix - assisted laser desorption ionization time of flight mass spectrometry ( maldi - tof ms ) after tryptic in - gel digestion
差異蛋白經胰蛋白酶酶切后產生肽片段，再利用基質輔助激光解吸電離飛行時間質譜得到肽指紋圖譜來鑒定。Sequence alignment shows that the fragment of pcr product showed some identities to the masp gene of xenopus laevis, branchiostoma belcheri, the trypsin gene in litopenaeus vannamei and the serine protease gene in aurelia aurita
Pcr反應產物基因片段大小為630bp ，序列比較結果表明， pcr克隆產物與爪蟾、文昌魚的masp基因，蝦、水母等的胰蛋白酶基因和絲氨酸蛋白酶基因都有一定的同源性。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。The cowpea trypsin inhibitor ( cptt ) gene is testified as a broad spectrum insect - resistant gene at present and its application in insect - resistant botanic transgenic engineering only after s / gene. the cpti transgenic plant developed rapidly for it ' s broad spectrum insect - resistant character and the target insects are uneasy tolerance to it
豇豆胰蛋白酶抑制劑（ cpti ）基因是目前在植物抗蟲基因工程中應用僅次於bt基因的廣譜性抗蟲基因。鑒於它抗害蟲的廣譜性和靶標昆蟲不易對其產生耐受性的優點，轉cpti基因植物得到了迅速的發展。The results as follows : 1 ) the primary culture of qinchuan - scalper skin cells could be derived by fragment of tissue dispersed and cold digested single cell, collegenase i ( 150iu ml - 1 " ) and trypsin ( 0. 05 % ) being used at the same time is best to dissociate qinchuan - scalper skin tissue, which is appropriate to obtain qinchuan - scalper skin cells
組織塊法、分散單細胞及dispase冷消化法單細胞均能獲得好的秦川牛皮膚組織原代培養物。其中150iu ? ml ~ （ - 1 ）膠原酶i與0 . 05胰蛋白酶（ 1 ： 1 ）同時應用能更好的分離秦川牛皮膚組織，是獲得秦川牛皮膚細胞的適宜方法。Huwentoxin xi ( hwtx ~ xi ), a serine protease ! inhibitor, consists of 55 amino acid residues with three disulfide bridges. the toxin was isolated from the venom of the chinese spider ornithochoctonus huwena by ion - exchange chromatogram - phy and reverse phase high performance liquid chromatography
本文報道從虎紋捕鳥蛛（ ornithochoctonushuwena ）粗毒中，應用陽離子交換和反相高效液相色譜的方法分離到一種胰蛋白酶抑制劑，命名為huwentoxin - （ hwtx - ） 。分享友人