腸膜菌素 的英文怎麼說
中文拼音 [chángmójūnsù]
腸膜菌素
英文
mesenterin-
Colicin ia belongs to the family of bactericidal proteins, the channel - forming domain of which can form lethal ion channel in the membrane of escherichia coli and related strains such as shigella sonnei
大腸菌素ia是可形成離子通道的一種細菌素,其通道結構域( d451 ? i626 )能在同種異株的大腸桿菌內膜上形成致死性離子通道。However, the receptor and translocation domains of the colicin ia can only recognize the inner and outer membrane receptors of the escherichia coli, therefore the wild type colicin ia can not be developed into antibiotics against other types of bacteria
然而由於其信號識別結構域只能識別大腸桿菌外膜和內膜上的特異受體,因此野生型大腸菌素難以拓展成為對抗它種細菌的抗菌素。Virulence factors of helicobacter pylori in patients with chronic gastritis, intestinal metaplasia or gastric cancer
幽門螺桿菌毒素與胃黏膜腸上皮化生和胃癌Thus, it is postulated that in the presence of mild neutropenia, whose function is inhibited by effect of insulin excess, the bacillus was able to find a port of entry, probably via micro - abrasions of the bowel mucosal lining
因此,推測可能存在中性粒細胞減少,功能被過多的胰島素抑制,芽孢桿菌可以乘虛而入,很有可能通過腸內壁粘膜微破損進入循環。The diaphragm had the ability to detect the positive serum when it was diluted to 2 ' 11 and so it has good sensitivity ; stored at 4 for at least 7 months, the sensitivity and specificity of the diaphragm did not change, so it has good stability ; when 10 positive serum was detected 3 times, the result is reproducible, so the diaphragm has good reproducible. serums from experimental inoculated piglets was detected. the results showed that when the titer is l : 16, the pigs were infected with streptococcus suis ; and when 1 : 64, the pigs could survive after challege with streptococcus suis. all the results have shown that dot - ppa - elisa was a convenient, rapid, sensitive specific useful method for the detection of antibody
該法以硝酸纖維素膜為固相載體,包被膜載抗原製成的診斷膜片具有良好的特異性:不與仔豬副傷寒、豬巴氏桿菌病、豬大腸桿菌病、豬衣原體病、豬瘟、豬細小病毒病、豬偽狂犬病、豬布氏桿菌、豬丹毒陽性血清反應;膜片具有良好的靈敏性,陽性血清作2 ~ ( - 11 )稀釋亦能檢出;膜片具有良好的穩定性,在4至少能保存7個月,其靈敏性不變。Clostridium bacteria form hardy spores, resistant to heating and drying. in poultry, clostridium perfringens causes necrotic enteritis, currently cured with antibiotics
在家禽中,產氣莢膜梭狀芽孢桿菌會造成腸壞死,目前使用抗生素進行治療。Physiological function, functional mechanisms of bifidobacterium and its applications in foods were investigated systematically by seven experiments in vitro and in vivo. they were composed of isolation and determination of bifidobacterium, comparison of growth characteristics, factors influencing survivability, bioantaganism to pathogen, adherence and colonization to mucus membrane, immunomodulation mechanisms on trial animals and development of products containing bifidobacterium
所以本課題通過體內及體外實驗方法,共計七個實驗,包括所研究菌的分離與鑒定;生長特性的研究;影響其存活因素的研究;對病原菌的生物拮抗:對腸粘膜的粘附與定植;對試驗動物的免疫調解機理以及含雙歧桿菌產品的開發,系統研究了該菌的生理功能與作用機理及其應用。The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體,分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒,並在大腸桿菌中誘導表達出相應的融合蛋白;用全長gstjqdrgz蛋白免疫兔,然後用gst ndrgz人和gstjqdrgze片段加強免疫,經免疫得到了較高效價的兔抗人ndrz多克隆抗血清,利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清,提高了ndrgz抗體的特異性;並對包涵體形式表達的6his ndrgz進行初步的分離純化。This project is aimed at : ( 1 ) expressing melittin and megf in e. coli respectively ; ( 2 ) constructing a membrane - toxic immunotoxin by using melittin as toxic part and megf as target part ; ( 3 ) measuring the megf mrna expression abundance by quantitative competitive rt - pcr ; ( 4 ) checking the genetic stability of the recombinant strains constructed in the project ; ( 5 ) optimizing the fermentation conditions of the target strains by flask shake
本課題以大腸桿菌( e . coli )宿主重組表達melittin和megf ;以melittin為毒性部分、 megf為靶向部分,構建膜毒性免疫毒素;用定量競爭rt - pcr法測定megf體內表達豐度;檢驗所構建的各重組菌株的遺傳穩定性;採用搖瓶分析對各重組菌株的發酵條件進行了初步優化。分享友人