自養菌 的英文怎麼說
中文拼音 [zìyǎngjūn]
自養菌
英文
autotrophe-
It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ) it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin
在疫苗生產實踐中,細菌污染是影響疫苗質量和產量的關鍵性因素,筆者通過了兩年左右的時間,選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份,進行細菌學檢查,並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗,結果表明:我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌,潛在威脅是桿菌和鏈球菌;強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果,可作為疫苗生產后備抗菌手段參考The nitrifying bacteria are chemosynthetic autotrophs.
硝化細菌是化能合成的自養生物。Autotrophic mutants were prepared from six different stocks of a special group.
自養突變體是從一個特別類群的6個不同的原種菌系制備的。The detection and application of nitrifying bacteria is limited by their peculiar ecophysiology such as strict autotrophic bacteria and slow growth
由於它們嚴格自養、生長緩慢的生態生理屬性,使硝化細菌的檢測和應用等受到限制。We mensurate the number of the primary soil microbes such as bacteria, fungi and azotobacter with diluting flat technique, and mensurate the number of cellulose decomposing bacteria with mpn technique, and mensurate the biological quantity of the soil microbes with the technique of chloroform suffocating - inundation culture - colour - compared
本文以長期壟作免耕試驗田為研究對象,採用稀釋平板計數法測定了土壤中主要的微生物細菌、真菌、自生固氮菌數量,用mpn (最大或然值法)測定纖維素分解菌的數量,採用氯仿熏蒸?淹水培養?納氏比色法測定微生物生物量。Wild filamentous fungi obtained from natural fermented millet catsup were identified as aspergillus oryzae by morphology after purifying iteratively
摘要反復純化培養自然發酵的粟米醬中分離的野生絲狀真菌,經形態學鑒定,確認其為米曲?菌群的米曲?菌。In this paper, the morphological characteristics of nine endophytes isolated from the barks, stalks, branches of taxus cuspidate sieb et zucc. grown in changbai nature reserve in northeast china ' s jilin province are studied through observation, description, shooting of culture - characteristics and microstructure
本論文通過培養性狀觀察、製片檢查,描繪及攝影等方法研究了分離自長白山自然保護區東北紅豆杉( taxuscuspidatesiebetzucc . )樹皮、樹莖和樹枝的9株內生真菌的形態學性狀。The number of mold, bacteria, culturable microorganisms, aerobic nitrogen - fixing bacteria, anaerobic nitrogen - fixing bacteria, aerobic cellulose decomposition microbes, anaerobic cellulose decomposition microbes were the most in 16 - year - old organic tea garden, and the biomass carbon and nitrification of edaphon were also the strongest
黴菌、細菌、可培養微生物總量、好氣性自生固氮菌、嫌氣性自生固氮菌、好氣性纖維分解菌、嫌氣性纖維分解菌均以16年生茶園最多,土壤微生物生物量碳和硝化作用也以16年生茶園最強。According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型,分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 ( de3 ) ,該工程菌株連續傳代100代表達質粒不丟失,表達量穩定;採用b . braun公司的biostat - c15l自控發酵罐,運用分批補料技術分別進行四種工程菌的高密度發酵,通過優化工程菌發酵的培養基配方及優化部分發酵條件(通氣量、攪拌速度、補料速度) ,最終建立一種適于目的基因高效表達的高密度發酵工藝模式。The nature of the nutritional requirements for obligate parasites in nature is unknown.
人們還不知道自然界中專性寄生菌的營養要求的本質。The brief analysis of marketing : because of the for foreigners use sodium to wood paper slurry blanch and the domestic use it to sterilize the water for living, fishing and swimming pools at present intead of chlorine to avoid the carcinogen caused by chlorine
市場簡要分析:國外氯酸鈉主要用於木漿造紙漂白,國內近年來主要用於自來水、游泳池、養魚塘的殺菌,替代傳統的氯氣,消除氯氣帶來的有害致癌物質。Based on the previous studies, the research in this paper was carried out, mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province. molecular techniques, such as fish, pcr, dna cloning and sequencing etc. were used for this purpose. ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied, which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process
本論文在前人研究的基礎上,開展了以下兩個方面的工作: ( 1 )採用分子生物學技術熒光原位雜交( fish ) 、多聚酶鏈式反應( pcr ) 、 dna克隆和測序等對采自江蘇省蘇州市、東太湖、新沂河等6個底質樣品進行了厭氧氨氧化菌和傳統氨氧化菌的原位檢測; ( 2 )探討了以底質作為接種體進行厭氧氨氧化菌富集培養的可行性,為天然底質環境中厭氧氨氧化過程的強化,富營養化底質微生物修復的可行性提供一定的依據。Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "
W12菌株對環境因子的適應性研究:無氮培養條件下,測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響,結果表明,在15 - 40下均能生長並表達固氮酶活性,其最適生長及固氮的溫度為25 - 35 ;能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮,當培養基中同時存在蔗糖和蘋果酸時,細菌生長和固氮活性最強;在偏酸和偏堿的條件下( ph4 . 5 - 8 . 5 )均能保持較強的生長勢和較高的固氮酶活性,並能通過調節自身代謝平衡並適應環境的酸、堿性變化,使培養液趨于中性:能耐受較高的滲透壓,培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大,當naci濃度升至2時,菌株的生長勢及固氮酶活性才有所下降:低濃度的鉸對其固氮酶活性影響不大,在0Conclusion a systematic method for preparation of enzyme - mannanse is established, a high productive strain was got after seducing and selecting from nature, confirmed as brachybacterium spa6 research were conducted on medium and culture method of the strain in order to get the suitable cultural condition of fermentation, the experiment result shows the optimium condition is ph7. 0, temperature 36c ; carbon content 2. 5 %, ventilation in abundence, agitation speed 200r / min
結論1 、以從自然界中篩選出的菌株為出發株,經誘變、篩選,得一高產葡甘聚糖酶菌株,初步鑒定為短桿菌屬brachybacteriumspa6 2 、經誘變、三角瓶培養,該菌株的最適培養條件:培養基ph值7 . 0 ,碳源2 . 5 ,振蕩培養, 200r min ,培養溫度36 ,培養48h 。The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination
本實驗將sod2基因構建到植物表達載體prok中,導入農桿菌后,進行植物遺傳轉化,實現其在擬南芥中過量表達,在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系,自交一代獲得足夠的純和轉基因種子后,對其進行了分子生物學的驗證及生理指標的檢驗。The kind of fungi fruit bodies develop on recently dead for intrusion or decayed plant materials. the lophodermium and hypoderma fungi collected from the huangshan mountains were isolated by tissue isolation techniques and culture characters data of 7 species were obtained
以組織分離法對采自安徽皖南山區的斑痣盤菌活菌材料進行分離培養,獲得7種散斑殼菌及皮下盤菌的培養性狀資料。Genetic diversity which was determined by the analysis of 16s rdna - rflp and 16s rdna - sequencing were carried out on frankia strains isolated from the root nodules of different actinorhizal plants, including myrica, casuarina, alnus and coriaria. the main results of the research project were as follows
本研究課題對來自楊梅( myrica ) 、木麻黃( casuarina ) 、榿木( alnus )和馬桑( coriaria )的13株frankia菌純培養的16srrna基因進行了pcr - rflp分析和部分菌株的序列測定,初步探討了弗蘭克氏菌的遺傳多樣性和系統發育地位。The results show that most isolates produce enough condia. which are similar to those directly received from the hosts, when they grow on the twa + w culture media for about 1 - 2 weeks, under 18 - 22 and alternative nuv light for 12h and in dark for 12h. but we should try several different kinds of culture media to produce the natural condia for some special species
總結如下: ( 1 )適宜培養基及適宜培養條件:使用自來水洋菜麥稈培養基( twa + w ) ,近紫外燈照射下, 18 22下, 12h光照與12h黑暗交替培養時,大部分菌落生長較好;但對個別的種也應使用多種培養基,選擇最接近自然狀態的培養基和培養條件。Glucose could obviously increase the flux of n2o among the carbon sources detected. that the methane - oxidizing bacteria probably were also one of microbiological populations producing n2o was proposed, based on research work. 2 ) methane oxidation ability of green - house soil was detected
研究也表明在自然界中除了硝化(包括自養和異養型)細菌和反硝化細菌種群以外,甲烷氧化菌可能也是對氧化亞氮釋放具有一定貢獻的細菌類群。The self - sustaining bacterial community, which thrives in nutrient - rich groundwater found near a south african gold mine, has been isolated from earth ' s surface for several million years
在南非金礦富養的地下水中發現的自養細菌群落,已經同地球表面隔離有數百萬年了。分享友人