色氨酸酶 的英文怎麼說

中文拼音 [shǎiānsuān]
色氨酸酶 英文
tryptophanase
  • 色 : 色名詞[口語] （顏色） colour
  • 氨 : 名詞[化學] （氮和氫的化合物） ammonia; hydrogen nitride
  • 酸 : 酸構詞成分。
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  1. Analysis of copper binding protein by sds - page, three main protein bands observed. the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc. 4

    Sds page分析得到3條主要蛋白帶，剪下這三條帶進行膠內賴氨酸內切酶的消化，通過高效液相色譜分離肽段，選擇性進行肽段的氨基酸序列測定。
  2. Phenylketonuria ( pku ) is an inherited metabolic disease that results in mental retardation and other neurological problems when treatment is not started within the first few weeks of life. the disease arises from the deficiency of a single enzyme, phenylalanine hydroxylase, which converts the essential amino acid, phenylalanine, to another amino acid, tyrosine. failure of the conversion to take place results in a buildup of phenylalanine in the body that then damages the central nervous system

    苯丙酮尿癥（ pku ）是一種智力發育不全的先天性疾病，患者由於肝贓內苯丙氨酸羥化酶缺乏，苯丙氨酸不能正常代謝為酪氨酸，從而導致苯丙氨酸在肌體組織內積累，引起腦損傷和累進性精神障礙，臨床表現為智力低下，頭發顏色轉黃，尿有異臭味，重者似鼠臭。
  3. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  4. Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly

    方法：通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型，主要採用抗fos 、膠質原纖維酸性蛋白（ gfap ）和酪氨酸羥化酶（ th ） （或加壓素? vp ）免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法，系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核（ son ）神經元? ast超微結構的變化、延髓內臟帶（ mvz ）和son及下丘腦室旁核（ pvn ）之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。
  5. It was found that the levels of catechins and proanthocyanins in those subclones accumulating anthocyanins were also raised, and were independent of the activity of phenylalanine ammonia - lyase

    發現積累色素的細胞系中，兒茶素與原花色素的形成能力也得到了大幅度提高，且與苯丙氨酸解氨酶活性無關。
  6. Research on tyrosinase, the key enzyme causing melanin formation, indicates that arbutin can effectively prohibite melanin formation by cubing on the activity of tyrosinase in non - cell body without affecting cell proliferation

    在體外試驗的非細胞體、對黑色素產生的關鍵酶酪氨酸酶的研究表明，熊果苷在不影響細胞增殖的濃度可以抑制黑色素的形成和使酪氨酸酶的活性降低。
  7. The results showed mn and ni complexes possibly bind to dna by the mode of interaction, whereas zn complex possibly bind to dna by the modes of interaction and electrostatic binding. 5. in addition, we conjugated cleavage system with recognize system and analyzed joint products by hplc, which provide experimental basic for design of dual effects cleavage

    此外，本文還選用咖啡酸純品來突破切割體系與識別體系（用氨基臂修飾的寡聚脫氧核苷酸）的連接，並用高效液相色譜法分析其偶聯產物，為今後設計併合成一種具有特異識別和高效切割雙重功能的人工核酸酶提供了實驗基礎。
  8. L - tryptophan is a neutral, genetically coded amino acid. iit is essential in human nutrition. formed from proteins during the digestive process by the action of proteolytic enzymes

    色氨酸是一種重要的中性必需氨基酸，在消化過程中，通過蛋白水解作用酶的運動而由蛋白質形成。
  9. After i. c. v. 6 - ohda, the c - fos expression of these areas induced by cold stress reduced significantly. 2 ) double staining showed that fos - like immunoreactive positive granules were observed in some vasopressin ( avp ) - immunoreactive positive neurons in paraventricular and supraoptic nuclei, as well as in some tyrosine hydroxylase ( th ) - immunoreactive positive neurons in nts and lc nuclei

    （ 2 ）雙重染色顯示，寒冷應激誘導的fos樣免疫反應陽性顆粒可見于室旁核（ pvn ）和視上核（ son ）中的部分加壓素（ avp ）陽性神經元及孤束核（ nts ） 、藍斑（ lc ）中的部分酪氨酸羥化酶（ th ）陽性神經元。
  10. Z which involve in melanin, tyrosinase related protein 1 gene ( tyrpi ) and dermal melanin inhibitor gene ( id ). the genomic structure of tyrp1 was determined and its correlation between melanin accumulation and tyrp1 expression was studied. moreover, we constructed a bac contig near id locus which was on a region short of dna marker in chr. z

    利用構建的bac文庫對z染色體上黑色素相關基因酪氨酸酶相關蛋白1基因（ tyrp1 ）的基因結構以及該基因的表達與黑色素沉積的關系進行了研究，同時構建了位於z染色體上缺乏標記區段的表皮黑色素抑制因子基因（ id ）的bac重疊群。
  11. According to the purity and the activity recovery, the recombinant enzyme was well purified by both of these two separation combinations. like natural gloshedobin, the recombinant enzyme exhibited strong esterase activity using tripeptide p - nitroanilide derivatives as substrate, but hydrolyzed n a - p - tosyl - l - arginine methyl ester ( tame ) or n a - benzoyl - l - arginine ethyl ester ( baee ) very weakly

    與天然蛇毒類凝血酶一致，當用三肽p山itroanilide衍生物作為底物時，分泌表達的重組大連蛇島蝗蛇毒類凝血酶具有較強的生色底物活性，但用精氨酸甲酯如tame （ na廠tosyl l ar 。
  12. Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively

    Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。
  13. Sleep / waking cycle is a complex network modulation and many factors such as interleukin - 1 ( il - 1 ), tumor necrosis factor ( tnf ), growth hormone releasing hormone ( ghrh ), vasoactive intestina polypeptide ( vip ) and many conventional neurotransmitters such as serotonin ( 5 - ht ), acetylcholine ( ach ), norepinephrine ( ne ), dopamine ( da ) and gamma - aminobutyric acid ( gaba ) were involved in it. recent evidence has shown that no synthesized in neurons in several areas of the brain can induce the release of neurotransmitters. in the rat central nervous system, the anatomical distribution of nos - containing neurons is now well established, and it was reported that nos is co - localized with neurotransmitters well known for their involvement in sleep mechanisms, i. e. 5 - ht, ach, da and gaba

    鄭州大學2003屆碩士畢業論文gaba受體激動劑消除no合成酶抑制劑對大鼠睡/醒周期的影響睡/醒周期的形成是一個復雜的網路調控的結果，體內許多因子都參與了這一調控網路，這些因子如白介素一1 （ il一1 ） 、腫瘤壞死因子（ tnf ） 、生長激素釋放激素（ ghrh ） 、血管活性腸膚（ vip ）以及經典的神經遞質如5一輕色氨（ 5一ht ） 、乙酞膽堿（ ach ） 、去甲腎上腺素（ ne ） 、多巴胺（ da ）和卜氨基丁酸（ gaba ）等，它們在睡眠的發生和調節中也發揮著重要作用。
  14. Although sds - page displayed that there were several protein bands in the sample after 16h fermentation, enzymatic active stain demonstrated that there was only one band during the whole fermentation period, indicating that there is only one kind alkaline protease in the fermentation liquid

    對發酵液進行sds - page和蛋白酶的活性染色分析表明，發酵液中的蛋白質種類不多，且只含有一種堿性蛋白酶。該發酵液所含蛋白酶活性能夠被絲氨酸蛋白酶抑制劑pmsf ， dfp完全抑制。
  15. Ag + and fe2 + caused the fluorescence of trp residues in g6pd quenched ; mg2 + and edta made fluorescence intensity of g6pd increased, this indicates that they caused trp residues wrapped and came to the inner core and located in the hydrophobic area ; while zn2 + or mn2 + made fluorescence intensity of g6pd decreased, this indicates that they made the conformation of g6pd relaxed and chromophores exposed to polarity environments. in native condition and in the far circular dichroic ( cd ) region, g6pd exhibited two characteristic negative band centered at 208nm and 222nm respectively, thus it is estimated to contain about 41. 2 % a - helix, 20. 6 % - pleated sheet and 38. 2 % random coil and turn

    Ag ~ +和fe ~ （ 2 + ）引起色氨酸（ trp ）殘基的熒光淬滅； mg ~ （ 2 + ）和edta均使g6pd的熒光強度增強，說明它們使trp殘基重新包裹在分子內部而處于疏水的微環境中； zn ~ （ 2 + ）和mn ~ （ 2 + ）均使g6pd的熒光強度變小，說明它們使酶分子構象變得疏鬆，原來處在分子內部的發色團暴露在極性環境中。
  16. Addition of iptg to growing culture of the lysogen induces t7 rna polymerase, which in turn transcribe the target dna in the plasmid. in the presence of glucose and appropriate conditions such as temperature and concerntration of iptg, a 52kd protein with tryptopanase activity was expressed

    摸索發酵條件，如改變培養溫度和iptg濃度等，發現在30培養條件下， 0 . 2mmiptg誘導時，發酵液中的吲哚含量最高，表明低濃度的誘導劑或低溫誘導有利於表達出有活性的色氨酸酶。
  17. Induced either by iptg or l - tryptophan in the absence of easily metabolized carbon such as glucose the strain bl21 ( de3 ) / pet28c - tnaa can express tryptopanase. the fermentation conditions were optimized respectively. in the presence of glucose and iptg as induce agent, the concentration is the crucial factor for expression of active tryptophanase. if the iptg concentration is less than 0. 2mm, the optimum temperature is 37 lower temperature is necessary to obtain active tryptophanase in the case of higher concentration of iptg

    用iptg誘導表達實驗結果表明：利用t7啟動子在胞內能表達出有活性的酶； iptg與溫度的合適組合可以得到較高活性的色氨酸酶，在0 . 2mm以下濃度的iptg誘導時， 37是最適溫度，而用較高濃度的iptg誘導，低溫是表達有活性的色氨酸酶的必要條件，而且誘導時間要短。
  18. Using pcr technology, a 2. 4kb dna fragment, part of tryptopanase operon, containing a promoter, a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e. coli k - 12, was cloned to pmd18 - t vector. the dna sequence is the same as which was published on science

    為了證明質粒上的基因能表達出有活性的色氨酸酶，將這個dna片段克隆到pet28c質粒的bamhi和hind位點上，使該片段受t7rna聚合酶的啟動子控制，然後轉化噬菌體de3的溶源菌bl21 （ de3 ） 。
  19. Abstract : the diferrences in cell growth, composition and levels of anthocyanins and catechins, activity of phenylalanine ammonia - lyase and dynamics of production between anthocyanin accumulating and non - accumulating subclones of tea cultured cells were examined

    文摘:從細胞生長、色素和兒茶素次生產物組成與含量、苯丙氨酸解氨酶活性以及產物合成動態分析幾方面，研究比較了積累色素與不積累色素的兩類茶樹培養細胞系之間的差異。
  20. This review focuses on the techniques and methods that are currently used for the detection of apoptosis, such as morphological observation, fluorescence staining, dna ladder, caspase activity, etc, and on the evaluation of the advantages and limitations of these methods

    本文綜述了常用的細胞凋亡檢測技術與方法的基本原理，如形態學觀察、熒光素染色、 dna階梯、半胱氨酸天冬氨酸蛋白酶活性的檢測等，並對各種方法的優點及局限性做一簡要比較。
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