芐化了的 的英文怎麼說
中文拼音 [huàlēde]
芐化了的
英文
benzylated-
As to the separation of p - xylene / m - xylene, in order to blend polymer hydrophobe with p - cd which is preferential to adsorb p - xylene, etherified p - cd was synthesized using benzyl chloride ( phch2cl ). the influences of etherification conditions on the reaction conversion and the degree of substitution of etherified p - cd were investigated. the reaction conversion increases with the increasing of molar ratio of phcr ^ cl : p - cd and the concentration of p - cd
對于px mx體系的分離,為使對px具有優先絡合能力的一環糊精( cd )能夠與油溶性高分子共混,本文採用williamson合成法運用氯化芐( phch _ 2cl )使-環糊精( - cd )醚化,並由元素分析結果,計算出醚化產物的取代度和轉化率,系統地考察了反應條件對轉化率和取代度的影響。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。The barnidipine hydrochloride were prepared via cyclocondensation from m - nitrobenzaldehyde, methyl acetoacetate and anmmonium bicarbonate, hydrolysis, esterification with opticaliy ( s ) - n - benzyl - 3 - py - rrolidinol, recrystallisation and salification
摘要研究了以碳酸氫銨,間硝基苯甲醛和乙酰乙酸甲酯為原料,經縮合、部分水解,再與( s ) - n -芐基3 -羥基吡咯烷經酯化、分步重結晶、成鹽得到鹽酸巴尼地平的工藝路線。The effects of both the ph values of extracts and the grinding of mycelia in liquid nitrogen on the result of dna extracting were also discussed
同時研究比較了液氮研磨和提取液的ph值對氯化芐法提取dna效果的影響。Benzyl choeide method, ctab method and sds - ctab method ) were used to extract dna from eleven strains of penicillium, the results showed that the benzyl choeide method was able to be used to extract the dna of those penicillium strains, and bands resulting from gel electrophoretic separations were very clear
比較了ctab 、 sds - ctab 、和氯化芐這3種dna提取方法提取青黴dna的效果。實驗結果表明: ctab法和sds - ctab法只能適合於部分青黴菌株的dna提取。Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product
用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ,根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物(上游引物: 5 ataggcatcatgggcgtctc3下游引物: 5 cagctaagcaaaacactccgc3 ) ,採用pyrobest ~ ( tm ) dnapolymerase (高保真dna聚合酶) ,通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物,將其與pmd18 - tvector連接后,轉化e . colidh5菌株的感受態細胞,經質粒抽提、酶切鑒定,確認該目的產物已得到成功克隆。Abstract : the application of carbonyl cobalt as a catalyst for carbonylation in organic synthesis is reviewed with 63 references. the carbonylation of benzyl chloride to phenylacetic acid catalyzed by carbonyl cobalt is discussed in great detail
文摘:討論了羰基鈷在有機催化羰化反應中的研究及應用現狀;介紹了羰基鈷催化合成各種有機羰基化合物的方法,重點討論了氯芐催化羰化合成苯乙酸的研究進展;在此基礎上對羰基鈷在催化羰化反應領域的應用作了展望。Au. the influence of several variables such as gold and cosolvent concentrations, shaking times, the ratio of tdmba and gold on the extraction of gold and the water concentration of the organic phase were studied. while the cosolvent ratio is more than 10 %, in the tdmba system, gold was extracted completely. the gold extraction mechanism was discussed on the bases of water concentrations of organic phases and infrared spectrum. it was found that the aggregation ratio of tdmba and au
等因素對萃取率的影響,並研究了有機相的連續萃取有機相金含量與水含量的關系。結果表明,助溶劑體積比10 %以上時,十四烷基二甲基芐基氯化銨對金具有萃取能力強,平衡時間短的特點。季銨鹽陽離子與au cnThe factors influencing on the yield of benzyl propionate, such as the dosage of catalyst, molar ratio of benzyl alcohol to propionic acid, reaction time and the amount of cyclohexane and so on, were investigated
考察了催化劑用量、苯甲醇與丙酸物質的量比、反應時間和帶水劑環己烷用量等因素對丙酸芐酯收率的影響。分享友人