芥菜苗 的英文怎麼說
中文拼音 [jiècàimiáo]
芥菜苗
英文
mustard cress-
S. regeneration system of mustard hypocotyls and cotyledons of 4 days and leaves of 25 days were used as transformation materials, and ms as basic medium supplying different ba ^ naa and kt concentration, we got the optimum explant adventitious bud inducing medium ( ms + 6 - ba3. 0mg / l + naa0. 2 mg / l ). among the three kinds of mustard explants. the cotyledons showed the highest adventitious bud regeneration capacity. and the regeneration capacity of leaf explantswas weaker. and the weakest of hypocotyls
芥菜高頻再生體系的建立以4天左右的葉用和莖用芥菜子葉、下胚軸和25天苗齡的芥菜無菌苗的葉片為外植體,以ms為基本培養基,通過加入不同濃度和不同組合的ba 、 naa 、 kt ,篩選到了外植體不定芽分化的最適培養基( ms + ba3 . 0mg / l + naa0 . 1mg / l )及生根的最適培養基( ms + naa0 . 2mg / l ) 。T. identification of charactrization of transgenic mustard plants the putative transformant regeneration plants were assayed by pcr and pcr - southern blot analysis. both analysis the target bands were observed. so the integration of the cpti gene into mustard genome dna was confirmed. the result of insect - resistance showed that the transgenic plants are more resistant than non - transgenic plants
轉基因芥菜植株的鑒定取再生苗的葉片提取dna ,進行pcr擴增和pcrsouthernblot分析,轉化再生植株大部分呈陽性,而非轉化的再生植株均為陰性,證明cpt基因已存在於芥菜基因組中。
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