苗期檢定 的英文怎麼說
中文拼音 [miáoqījiǎndìng]
苗期檢定
英文
seedling test- 苗 : 名詞1 （初生的種子植物） seedling; sprout; shoots 2 （初生的飼養動物） the young of some animals ...
- 期 : 期名詞[書面語]1. （一周年） a full year; anniversary 2. （一整月） a full month
- 檢 : Ⅰ動詞1 （查） check up; inspect; examine 2 （約束; 檢點） restrain oneself; be careful in one s c...
- 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
- 苗期 : [農業] seedling stage苗期管理 seedling management
- 檢定 : docimasy; docimasia; verification; calibration; appraisal檢定報告 probation report; 檢定滴定管 [...
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With the successful expression of exogenous gene in plant. the advantage of plant expression system become a highlight increasingly. based on the successful expression of hbmp - 3m gene into tobacco, we maked the study o f transferring hbmp - 3m gene into tomato and hbmp - 3 gene into tobacco, in order to obtain tomato transgenic plant with hbmp - 3m gene and tobacco transgenic plant with hbmp - 3 gene, to establish basis for getting step farther of leaning the expression of hbmp - 3m gene and hbmp - 3 gene in plant, the difference between the product of hbmp - 3m gene and hbmp - 3 gene in plant and the active of expressible product
隨著植物基因工程的迅猛發展，一些疫苗、抗體、細胞因子等異源蛋白在植物中的成功表達，植物表達系統的優點日益受到關注。本研究室在成功地將hbmp - 3成熟肽基因轉入煙草並檢測有目的蛋白表達的基礎上，進行hbmp - 3成熟肽基因轉化番茄和hbmp - 3全長基因轉化煙草的研究，以期獲得轉hbmp - 3成熟肽基因番茄和轉hbmp - 3全長基因煙草植株，為進一步研究hbmp - 3成熟肽基因和全長基因在植物體內的表達及區別奠定基礎。In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
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