蛋白帶 的英文怎麼說

中文拼音 [dànbáidài]
蛋白帶 英文
chalaza
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 白帶 : [醫學] leukorrhea; leukorrhoea; leucorrhea; whites白帶過多 leukorrhagia
  1. The target gene was then subcloned into plasmid vector and induced by iptg for its expression. after that, the recombinant protein was purified and used for the identification and characterization of its immunogenicity. the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated

    Niptg誘導表達，產物沉澱中於約45kda處顯見高合蛋白表達帶， westernblot分析表明，該蛋白帶可被篩庫血清中特異性lge抗體識別；而載體本身表達的26gst蛋白帶則否。
  2. 4 hemolymph immune reaction of p. americanna after nature infection with m. anisopliae dripping the cockroach body with m. anisopliae showed that the m. anisopliae spores can invade into the hemolymph coelom of p. americanna through cockroach cuticle and produce blastospores

    美洲大娩對金龜子綠僵菌cqmal02菌株的免疫反應經sds page電泳發現，注射誘導后的蜚蛾血淋巴在43kda處出現了一條新的蛋白帶，但含量很低。
  3. The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form

    本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。
  4. Analysis of copper binding protein by sds - page, three main protein bands observed. the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc. 4

    Sds page分析得到3條主要蛋白帶，剪下這三條帶進行膠內賴氨酸內切酶的消化，通過高效液相色譜分離肽段，選擇性進行肽段的氨基酸序列測定。
  5. Meanwhile, the results show that sp - d, sp - j, sp - z and sp - c are relatively tolerant to lincomycin, but the other 6 strains of meterials are not

    7kd的一條蛋白帶，而直線形藻絲體即mo則缺失此帶，表明此53 7l蛋白與螺旋藻的形態變異相關。
  6. Sds - page results showed that there was a clear target protein band in mut + recombinant supernatant after 48 hours of culturing, while a faint band only in muts recombinant after 72 hours. western - blotting result showed that there was no remarkable difference of yield between mut + and muts recombinants after 6 days induced. anti - virus activity tests revealed that culture supernatants of mut + and muts recombinants could inhibit tmv infection with high efficiency in the same concentration and there was no significant difference between them

    結果表明，誘導培養48小時后， mut ~ +重組菌株表達產物在sds - page膠上顯現出清晰的目的蛋白帶，而mut ~ s重組菌株培養72小時才能顯示微弱的目的帶； western - blotting雜交信號強度表明，同樣培養6天的mut ~ +和mut ~ s重組菌株表達產物在表達量上沒有明顯差別。
  7. The purity was detected by sds - page, and only igg ' s heavy chain and light chain protein tope were showed up. the activity of igg was tested by technique of immune credeschs gold

    用sds - page進行igg的純度檢驗，電泳結果出現兩條蛋白帶，是igg的重鏈和輕鏈，說明純化的程度較高。
  8. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  9. But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation

    將表達產物破包涵體后經glutathinonesepharose4b柱親和層析分離， sds page顯示純化的蛋白質為一分子量60kd的單一蛋白帶。
  10. Materials and methods in our study, first the e. coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis. the plasmids dna extraction were processed 37 overnight by the restrictive endo - incisase bam h i and xho i. the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec - trophoresis

    同預想結果基本吻合，大規模培養后純化得到的融合蛋白通過sds page顯示在52kda處有一條帶，而酶切后產物電泳結果顯示在26kda處分另有兩條蛋白帶，其中一條為gst ，另一條為hbrp ，基本符合實驗結果； hbrp對tpk活性的抑制呈劑量依賴性。
  11. The purified lectin showed a single protein band on sds - page, and the subunit molecular weight was 12kd. the molecular weight was 45kd determined by gel filtration on sephacryl s - 100. the result implied that there are four same subunits per lra

    經sds - page檢測為單一蛋白帶，亞基分子量為12kd ， sephacryls - 100凝膠過濾測得其表觀分子量為45kd ，表明lra是由四個相同亞基組成的蛋白。
  12. A 40 000 protein was proved to be their common omp antigen, whereas the specific omp antigen of the four strains was a 43 000, 50 000, 38 000 and 50 000 protein, respectively. finally, 20 mice were immunized with the omps of a type strain j - l ( 50, u < gper mouse )

    這些表明了不同表型種嗜溫氣單胞菌的omp型存在一定的差異，而同一表型種的不同菌株間的omp型盡管相似，但蛋白帶的遷移率及顏色深淺卻仍有差異。
  13. On d2 after attachment, the protein bands attained 23. after mating, the protein bands reached 26 and maintained the same until d4 after engorgement. these results meant that feeding and mating stimulated the biosynthesis of new protein

    饑餓雌蟲唾液腺有17條蛋白帶：吸血后2d增加到23條；交配后達到26條，直到飽血后4d保持不變，吸血和交配能刺激新蛋白的合成。
  14. 9 expression of mxmybl gene in e. coli was experimented. the structure of its recombinant expression vectors had not base mutant and drift. sds - page of pet - mxmybl proteins showed that there was a strengthened protein band in expectant 38kda protein marker

    構建的原核表達載體結構正確，未出現堿基突變及移碼現象； lmmoffeiptg誘導zh后，在預期的蛋白分子量約38kda處出現一條表達加強的蛋白帶。
  15. And we have also studied its expression in protoryotes and the conserved regions of insect chitinase genes in different insects pests

    Sds page電泳顯示，在50th附近沒有特異蛋白帶的出現，此結果表明外源基因沒有得到表達。
  16. In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

    為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
  17. On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction

    本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
  18. The target proteins with molecular weights of 35kd and 44kd were detected by sds - page. the 35kd target protein recovered from sds - page was used to immunize rabbit and the annsemm with high titer was obtained

    Annfaf基因在大腸桿菌中以包含體的形式得到了高效表達， sds - page檢測表明，分別獲得44kd和35kd大小的蛋白帶，與預期結果相同。
  19. Purified glycoprotein was detected by gelcodeglycoprotein staining kit, and the result indicated that it was a glycoprotein, because two glycoprotein bands appeared in gel, with their molecular weights 63000 and 54000 respectively

    糖蛋白染色鑒定試劑盒鑒定結果表明： cona - sepharose4b親和層析的茶樹葉蛋白是糖蛋白，在sds膠上出現了兩條糖蛋白帶，其分子量分別為63000和54000 。
  20. After sequencing the vectors were transformed into e. coli dh5 a and recombinant fusion protein was expressed via induction of iptg. fusion protein coded by carboxyl terminal gene sequence was purified through glutathione agarose column

    經sds page分析，在相對分于質量（ mr ）為74000和43000處，各有1條特異的蛋白帶。
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