蛋白粒 的英文怎麼說
中文拼音 [dànbáilì]
蛋白粒
英文
protein grain-
First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的:構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后,利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素,初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。Aleurone grain ( aleurone body ) a modified vacuole found in the embryo and endosperm of seeds and containing mostly reserve proteins, but also phytic acid and various enzymes associated with mobilization ( digestion ) of these reserves
糊粉粒(糊粉體) :在種子胚或胚乳中發現的經過修飾的液泡,裡面包含許多儲藏蛋白,植酸和各種各樣的酶。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Composite of autologous morselized cancellous bone grafts with bone morphogenetic protein in repairing bone defect
自體微小顆粒骨復合牛骨形態發生蛋白修復骨缺損Negatie results of tests for ceruloplasmin, antinuclear antibodies, and antimitochondrial antibodies do not definitiely rule out wilson ' s disease, autoimmune hepatitis, or primary biliary cirrhosis, respectiely ; howeer, these diseases do not adequately explain the other features of this patient ' s presentation
血漿銅藍蛋白、抗核抗體和抗線粒體抗體檢查結果陰性並不能排除肝豆狀核變性、自身免疫性肝炎或原發性膽汁性肝硬變,然而,這些疾病各自並不能充分地解釋該患者的其他臨床表現。A small cryptical plasmid pefr was isolated from enterococcus faecium strain df101. the complete sequence analysis of the plasmid show that it consists of 3176 bps, which contains four putative orfs. orf1 encodes a putative protein and is highly similar to repa which functions in replication
從屎腸球菌df101菌株中分離到隱秘的小質粒pefr ,全序列分析顯示質粒pefr由3176bp組成,編碼四個推定的orf , orf1編碼的一個推定的蛋白和復制有關的repa有很高的相似性。The majority of mitochondrial proteins are encoded by nuclear genes, synthesized in the cytosol and subsequently imported into mitochondria
摘要線粒體的大多數蛋白質是由核基因編碼、細胞質合成,而最終運輸到線粒體。Results : folate - conjugated bsanp ( folate - bsanp ) was successfully achieved
結果成功制備了葉酸偶聯白蛋白納米粒。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into
經限制酶消化和dna序列分析,證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列,在iptg誘導下兩種嵌合分子都獲得了分泌表達,表達產物主要集中在細胞周質空間。The highest antiviral litre of rpoifn - a was l l08iu / mi. the rpoifn - a protected pk - 15 cells against virulent hog cholera virus ( hcv ) infection in vitro
Sds - page分析表明,構建的重組表達質粒pqe30 poifn在大腸桿菌jm109中表達的rpoifn占菌體蛋白總量的20 26 。Expanded bed adsorption ( eba ) is a novel bioseparation technique, which integrates clarification, concentration and initial purification into a single unit operation. it enables proteins to be recovered directly from unclarified cultivations of microorganisms or cells and homogenates of disrupted cells, without the need for prior removal of suspended solids. matrix is the principal " hardware " pillar supporting the successful application of eba
擴張床吸附( eba )技術是一種新型的生化分離技術,它集成了固液分離、濃縮和初期純化於一步單元操作之中,可以直接從含有細胞和細胞碎片的發酵液或培養液中提取目標蛋白,而不必事先除去懸浮的固體顆粒。By using these antibodies, which were raised to immunoreact with total proteins of purified mitochondria from different organs of mung bean seedlings, we find that there were two hybridizable aox bands in mitochondria in mung bean seedlings. their molecular weight was about 35kd and 38kd respectively
將此抗體與來自綠豆幼苗不同器官線粒體的總蛋白分別進行western雜交,可觀察到綠豆幼苗線粒體中具有兩條清晰的aox雜交帶,它們的分子量分別為35kd和38kd 。That mitchondria in interspecific hybrids become molecular mosaics containing proteins of both species.
種間雜種內這些線粒體變成含有兩個種屬蛋白質分子的鑲嵌物。The effect of transforming growth factor - 1 on expression of smad3 and smad7 in keloid - derived fibroblasts
異體脫鈣骨基質骨粒骨水泥骨形態發生蛋白復合材料成骨誘導活性Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively
本實驗用sds - page電泳和免疫印跡的方法,用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定,結果表明:抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應,同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在,表明在植物中有類三磷酸肌醇受體蛋白的存在;用免疫膠體金方法,發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。They have also been isolated from mitochondria
目前已從線粒體中分離出鐵硫蛋白。Cloning and expression of neutrophil activating protein gene of helicobacter pylori
幽門螺桿菌中性粒細胞激活蛋白的基因克隆與表達1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out
具體包括以下幾個部分: 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議,根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究,對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述,分析系統學研究中存在的不足,提出了雁形目鳥類分類中急需解決的問題。Additional oral sources of bad breath include poor oral hygiene ( especially if it leaves proteinaceous particles between teeth ), gum inflammation, faulty dental work, unclean dentures, and abscesses
其他的口腔問題也會引起口臭,包括不良的口腔衛生習慣(尤其是牙縫間殘留蛋白質食物顆粒) 、牙齦發炎、不當的牙齒修補、不潔假牙以及膿瘡。分享友人