蛋白結合測定 的英文怎麼說

中文拼音 [dànbáijiēgěcèdìng]
蛋白結合測定 英文
protector binding assay
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 結 : 結動詞（長出果實或種子） bear （fruit）; form （seed）
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 測 : 動詞1. （測量） survey; fathom; measure 2. （測度; 推測） conjecture; infer
  • 定 : Ⅰ形容詞1 （平靜; 穩定） calm; stable 2 （已經確定的; 不改變的） fixed; settled; established Ⅱ動詞...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  • 結合 : 1 （發生密切聯系; 聯合） combine; unite; integrate; link; binding; coalition; cohesion; connectio...
  • 測定 : determine; determination; setting-out; admeasurement; assignment; assay; finding
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. The researchers combined proteomics ( the study of proteins ), the latest in mass spectrometry technology and the best analytical methods from the field of bioinformatics ( the use of computers and statistics to analyze and find patterns in scads of data )

    研究者將蛋白組學（研究蛋白質） ，最新的質譜測定技術及最好的生物信息學分析方法（應用電腦及統計學分析並檢驗大量數據類型）相結合。
  3. After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells, the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope, indirect immunofluorescence and flow cytometer analysis. prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed

    建系細胞培養上清與肝癌細胞作用后，經熒光顯微鏡觀察、間接免疫熒光及流式細胞儀檢測進一步確定表達的scfv融合蛋白具有與hbsag特異性結合的活性。
  4. In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization

    本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。
  5. A piezoelectric immunosensor was developed by immobilized transferring antiserum on the surface of quartz crystal to especial answer transferring, it can be get good answer to determined in the range of 3. 92 ~ 79mg / l ; the adsorption and desorption of consult serum on the quartz crystal monitored with tr - antibody, determined the rate constant of immuno - reaction was 2. 14xl07 ( mol / l ) ' 1xs ' 1, the association constant of the immuno - reaction ( k1ss ) was 6. 75xl07 ( mol / l ) ~ l

    Oz vomg l范圍內的參考血請中的轉鐵蛋白進行了定量測定，獲得很好的響應；利用石英晶體微天平技術在線監測了參考血清在固定了轉鐵蛋白抗體的石英晶振上的吸附與解吸過程，測得了其抗原抗體免疫反應的速率常數為2 14xl0 』 （ mol l ） 』 xs 「 『 ，結合常數為6
  6. This paper stuffed with twelve important grain and vegetable crops, studied the injury symptom, dose reaction, injury threshold value and influential factor of main pollutant so2 on various plants, tested the dynamic transformation of pod, cat, mda, soluble protein, free pro and chlorophyll of resistant plant and sensitive of these physiological biochemical transformation with plant resistant ability. meanwhile, simply studied the protective role of the five compounds on plant. the result indicated the followings

    本實驗以12種重要的糧食和蔬菜作物為研究對象，研究了主要大氣污染物二氧化硫（ so _ 2 ）對不同植物的傷害癥狀、劑量反應、傷害閾值以及影響因素，測定了抗性和敏感植物在受到so _ 2污染后植物體內過氧化物酶（ pod ） 、過氧化氫酶（ cat ） 、丙二醛（ mda ） 、可溶性蛋白質、游離脯氨酸和葉綠素的動態變化，並分析了這些生理生化變化和植物抗性的相互關系，同時還對5種化合物溶液對植物的保護作用進行了初步研究，結果表明： 1
  7. So piezoelectric immunnosensors is gradually applied to some fields such as biochemical research, clinical analyze, environment and industry process assay. according to different method of immobilized antigens or antibodies, developed some new piezoelectric immunnosensors to determine proteins in consult serum

    湖南大學分析化學碩士論文李丹200年6月本文結合壓電免疫傳感器抗體（抗原）固定化方法的不同，研製了一些新的壓電免疫傳感器用於參考血清中的一些蛋白的測定。
  8. In order to check if it is the aim gene, we devised pcr with a new pair of primer, sequenced and registered the product with registration number : af449446. moreover we forecast and analysis the primary, secondary, tertiary and quaternary structure of the three protein : osftszi, crftszi and crftsz2 which has already cloned by our team before. after that we construct ftsz molecular evolution tree to site them in

    又將生物信息學技術同實驗技術相結合，針對ftsz保守區設計引物擴增出一條衣藻ftsz片段，進行est搜索、比對、拼接，最終克隆出新基因crftsz1 ；連同本試驗室曾經獲得的另一個衣藻crftsz2基因進行蛋白質的一、二、三、四級結構預測、分析及比較尋找進化線索，建立了ftsz蛋白的氨基酸進化樹作進一步的進化定位。
  9. Part i this paper has minutely studied the interaction between ag ( i ) and serum albumin. the binding of ag ( i ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 5. 4 ). the scatchard analysis indicates that there exists several strong binding sites of ag ( i ) in both hsa and bsa. a notable hysteretic effect has been observed in the interaction of ag ( i ) with hsa or bsa using uv - visible spectrometry at ph ( 5. 4 ), which shows that the binding between ag ( i ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for ag ( i ) to one of stronger affinity ( a - b transition ). the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed. the binding equilibrium has been also studied by resonance light - scattering spectrum ( rls ) and flurescence quenching

    第一部分：等離子點ph （ 5 . 4 ）條件下，用平衡透析法和紫外光譜，熒光光譜，共振散射光譜研究了ag （ ）與人血清白蛋白（ humanserumalbumin ，簡稱hsa ）或牛血清白蛋白（ bovineserumalbumin ，簡稱bsa ）的結合。 scatchard圖分析表明， ag （ ）在hsa或bsa中有強弱兩類結合部位，通過計算機擬合獲得結合的逐級穩定常數值。紫外掃描發現ag （ ）與hsa或bsa的結合存在滯後效應，表明ag （ ）與hsa或bsa的結合可能誘導蛋白質構象發生緩慢變化（ a - b ） ，測得並討論了這一構象變化的速度常數和活化參數。
  10. In addition, the well retained stability and integrity of cell membrane of boea leaves might also be an important mechanism which make them resurrect well. by using mrna differential display, 5 desiccation sensitive cdnas, 52 desiccation - induced cdnas, 21 up - regulated cdnas, 14 down - regulated cdnas and 16 phosphate induced cdnas were obtained. the cloning, sequencing, homological blasting and northern blotting results of 5 desiccation - induced cdnas and 3 phosphate induced cdnas implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as g protein, protein kinase, vp3 - and mad3 - like genes might be involved in dehydration in the resurrection plant boea hygrometrica

    對其中5個脫水特異誘導表達牛耳草光合作廠j的脫水保護和復甦機理的cdna （包括可能與復甦能力有關的cdna ）和3個磷酸鹽處理誘導表達的cdna進行克險測序、同源性探測和northern雜交檢測表明，牛耳草脫水過程中誘導表達的基因可能涉及到脫水脅迫的信號轉導「蛋白、蛋白激酶等） 、調節基因的級聯作用（ vp3 ， mad3樣基因等） 、結構基因產物調節細胞結構（包括細胞質膜）在脫水脅迫中的穩定性等。
  11. We have demonstrated isolation of single - class plasma lipoproteins based on the immunological method associated with streptavidin ? biotin capture technology, and the lipoprotein cholesterol was consecutively quantified on the same strip without handling of reagents ( 11 )

    我們還論證了基於免疫學方法結合鏈親和素捕獲技術分離單一級別血漿脂蛋白，並且在同一條帶上不用對反應物進行操作就能連續定量檢測脂蛋白膽固醇。
  12. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  13. Ptxb1 - hng and ptxb1 - m - insulin are expressed in e. coli successfully. after sds - page and densitometric scan analysis, the results show that the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % of total bacterial proteins. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody ( igg )

    Pbv220 ? hng在大腸桿菌中未檢測到表達，后兩個克隆在大腸桿菌bl21 （ de3 ）中獲得高效表達， hng及m - insulin融合蛋白表達量分別佔全菌蛋白的40及50左右；經western - blot鑒定m - insulin融合蛋白可以與小鼠抗人胰島素單克隆抗體（ igg ）發生抗原抗體結合反應。
  14. Recently an abscisic acid binding protein with high specificity and affinity for aba was purified from epidermis of vicia faba l. in our laboratory, and further characterized using various biochemical techniques, there is evidence to link the protein to the physiological effects of aba in some aspects. the above - mentioned work may result in the molecular cloning the gene encoding aba binding protein

    近年來，我室利用親和層析從蠶豆下表皮中分離出一種高親合、高度特異性的aba結合蛋白，並完成了對其生化特性深入分析及部分測序，且在一定程度上揭示了該蛋白與aba的生理功能效應存在著內在的聯系，這為進一步克隆aba結合蛋白基因奠定了堅實的工作基礎。
  15. So the method was accurate and reliable. the in situ nile blue ( nb ) dimer with weak fluorescence in the solution of anionic surfactant sodium dodecylbenzene sulfonate - 6 ( dbss ) was also used as the fluorescence probe for the determination of total proteins in human serum

    以陽離子染料耐爾藍在陰離子表面活性劑十二烷基苯磺酸鈉存在下形成的現場弱熒光二聚體作為測定人血清總蛋白的熒光探針。該法用於實際樣品的測定，與臨床方法測定的結果相吻合。
  16. In order to get some functional clues from their structures, the upstream regulation region of ndrgl gene and second structure of ndrg2 protein are performed bioinformatics analysis ; we found that there are several binding sequences of some diffirent transcription factors, their functions include regulating tissue - specific gene expression, regulating expression of genes related to growth and early development of cells, besides this, regulating expression of genes under some stimulated conditions, and so on. predict in protein fold classification shows that ndrg2 belongs to alpha / beta hydrolase fold family, and there are high similarity between ndrg2 and epoxide hydrolase from bacteria, this suggests that ndrg2 protein may has enzymatic functions associated with resisting the oxidative stress, maintaining the balance of cell redox potential, involving in the metabolism process of xenobiotics or intracellular toxic molecules

    研究發現呷基因的調控區存在多種轉錄因子結合位點，功能主要涉及組織特異性表達調控，細胞生長發育相關基因的表達調控，刺激反應基因的表達調控等； ndrgz蛋白在結構上屬于a小水解酶類折疊，折疊分類預測表明ndrg2與其中的的細菌環氧化物水解酶的二級結構極為相似，提示ndrgz蛋白具有一定酶活性，可能參與細胞抗氧化應激反應，維持細， an ） armtbffiofbfochmilsyn ） mdafblechmrbfobo4第四軍醫大學碩士學位論文胞內氧還電勢平衡，參與內外源有毒物質的代謝等。
  17. The cdna expression library that contains no intron theoretically include all expressed genes and conserve resource genes permanently. lt can be used to find out and clone some genes which can express particular protein with modern molecular biological techniques, such as immunological screening, drug - prob screening, southern et al. lt is very important to study the life nature of plasmodium falciparum in molecular level. with the developments of these studies, the drug - resistant mechanism of the plasmodium falciparum and the genes of some specific medicine binding protein can be made well - known. at the same time, the researches will do good to explain the mechanism of some specific medicines in order to design and screen new anti - malaria drugs

    建立cdna表達文庫在一次永久保存基因資源的同時，可以利用功能篩選、免疫學篩選、藥物探針篩選、 southern雜交和大規模序列測定等現代分子生物學技術尋找特異性活性蛋白基因，進而克隆和表達這些基因，對從核酸及蛋白質等分子水平研究瘧原蟲的生命活動規律，對揭示其抗藥性分子機理，搞清某些特效藥物結合蛋白的基因及此類藥物的作用機制，對新型抗瘧藥物的合理設計及篩選都具有極其重要的現實意義。
  18. 2. a bhlh - type gene osbhlhl was obtained from the rice cdna library by using the rapid cloning pcr. the predicted osbhlhl protein had a nucleus location signal, a putative dna binding domain contained a bhlh - bzip domain

    2利用pcr篩庫法，從水稻cdna文庫中克隆到一個bhlh類型的基因，命名為osbhlh1 ，它的推測蛋白結構中含有一個細胞核定位信號和一個可能的與dna結合bhlh - zip結構域。
  19. These results showed these proteins have a high degree of similarity ; they are basic and cysteine - rich proteins with a signal peptide and a common pattern of eight cysteines that engaged in four disulphide bridges holding together four a helices and stabilizing the structural fold. a hydrophobic central cavity in which can occupied by lipids is found between the four helices. however, it has been difficult to draw any conclusions about the in vivo activity of nsltps from their lipid binding properties because it is unknown which ligands, if any, are bound to nsltps in vivo

    不同物種的非特異性轉移蛋白具有很高同源性，它們是堿性的富含半胱氨酸的蛋白質，在n端有一個信號序列， 8個保守的半胱氨酸殘基能形成4個二疏鍵以維持蛋白的空間結構，推測在其空間結構的中心形成了一個能容納脂類物質的洞穴，但在體內還不知道nsltp的配體包括哪些物質，對于nsltp能否在體內能夠結合併轉運脂類還沒有明確的定論。
  20. Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage

    構建了echistatin融合蛋白表達載體，經dna測序鑒定正確后，溫控誘導表達，獲得了高效表達，表達產物經505一隊ge分析，分子量為16000oa ，符合預計的融合蛋白分子量，表達產物. ll 』菌體總蛋白的30 %以上， westem一blotting結果顯示，該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應，表明我們成功構建了eohistatin的高效融合表達克隆。
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