融合基因 的英文怎麼說

中文拼音 [rónggějīyīn]
融合基因 英文
fusion chain
  • 融 : Ⅰ動詞1 （融化） melt; thaw 2 （融合; 調和） blend; fuse; be in harmony Ⅱ形容詞[書面語]1 （長遠; ...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 融合 : fuse; mix together; anastomosing; reconcile; harmonize; compromise; amalgamate; coalesce; coalesc...
  1. Sodium succinate as carbon source could lead to earlier sporulation and earlier expression of cryld - lacz, when sodium acetate acted as carbon source, cryid - lacz could keep high, stable expression in different hosts

    琥珀酸鈉作碳源時導致芽胞形成提前， cry1d - lacz融合基因表達也隨之提前。而以乙酸鈉作碳源時，在不同亞種的菌株中都能高水平、持續穩定地表達。
  2. The blood of each rabbit was collected at days 0, 7, 14, 2l, 30. the antibody titers were evaluated by k - agglutination test. the results showed that lower agglutination titers was observed at day 7 and up to higher levels over 8000at day 21 induced by the vaccine of recombinant fused protein

    將融合基因工程菌株在營養肉湯中培養，離心後上清用0 . 1mmgcl _ 2沉澱，離心，提取表達產物。經sds - page電泳證明表達產物為纖毛蛋白與il - 2的融合蛋白。
  3. To target this mitochondrial enzyme into chloroplast, the cdna sequence of mnsod was fused to a chloroplast transit peptide from a pea rubisco small subunit gene, whereas expression of the chimeric gene was driven by the camv 35s promoter

    Pchlsod質粒含有煙草mnsod基因的cdna序列，與豌豆核糖體小亞基葉綠體引物肽（ tp ）的編碼基因序列構成融合基因，由35s啟動子調控。 npt基因為選擇標記基因， pgv2260為輔助質粒。
  4. To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated

    但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。
  5. Css is the one of the colonization factor antigens which is a protective antigen can cause immune reaction by the means taking orally. in this study, carrot was separately transformed with agrobacterium tumefacience strain lba4404 that contains ctb or ctb - cs3 fused gene in order to get oral diarrhea vaccine. this is a potent strategy to produce etec oral vaccine

    本研究期望通過農桿菌轉化系統將ctb （霍亂毒素b亞基，可作為佐劑和載體）基因和ctb - cs3融合基因分別轉入胡蘿卜植株，使ctb和ctb ? cs3融合基因穩定整合到胡蘿卜基因組內，希望以此獲得以胡蘿卜為受體的etec口服疫苗，使服食者在進食的同時就可獲得腹瀉免疫。
  6. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  7. In our study we have cloned the osd gene from s. typhimurium by pcr, characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd

    再將hpylori尿素酶b亞單位基因與尿素酶b和熱休克蛋白a融合基因分別克隆入ptrc99a一asd質粒的多克隆位點之內。
  8. ( 4 ) in order to investigate the influence of the carbon source on the expression of cryld - lacz, cryld - lacz was introduced into different strains and cultured in liquid medium containing different carbon sources

    檢測了在蘇雲金芽胞桿菌不同菌株中，不同碳源對cry1d - lacz融合基因表達的影響。
  9. Besides, vgb gene expression also increased the chitinase secretion. in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis, an inducible promoter ( levansucrase ) was cloned from b. subtilis wb600 and ligated with a promoter - less vgb gene. the resulted gene is called sacvgb and was demonstrated to express in e, coli by sds - page and carbon monoxide binding assay

    由於vgb基因啟動子不能在枯草桿菌中啟動表達，因此，根據已發表的果聚糖蔗糖酶基因（ sacb ）序列設計引物，從枯草桿菌wb600總dna中擴增出該基因的啟動子片段，然後將其與vgb基因編碼區及終止子序列相連，成功地組建了sacvgb融合基因。
  10. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  11. In the third part of the thesis, a chlamydomonas reinhardtii chloroplast expression vector, pactbvpl, containing the fusion of the foot and mouth disease virus ( fmdv ) vp1 gene and the cholera toxin b subunit ( ctb ) gene was constructed. transformation of c. reinhardtii chloroplast was achieved by biolistic bombardment with pactbvpl

    論文第三部分主要敘述了將o型fmdvvp1與強黏膜免疫佐劑霍亂毒素b亞基（ ctb ）的融合基因克隆重組到衣藻葉綠體表達載體中，並採用基因槍法轉化衣藻葉綠體，獲得了具有壯觀黴素抗性的轉化子。
  12. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  13. The influence of cellular immunity and genetic factor on the effect of mother to infant transmission interrupted with hepatitis b vaccine

    6融合基因在恥垢分枝桿菌中的表達及其抗原性的初步研究
  14. Based on the foundation research of the interaction of virus and host actin, we recombine gfp gene - a reporter gene - with 5c actin gene of drosophila melanogaster, a gfp - actin fusion gene was obtained

    本文在總結前人關于病毒與宿主肌動蛋白相互作用的研究的基礎上，利用綠色熒光蛋白基因為標記基因，與果蠅肌動蛋白基因5cactin基因融合，構成gfp - actin融合基因。
  15. In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia

    並以已構建的載體ptyb2 - mae為模板，通過pcr擴增出融合基因mae - imein - cbd ，將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  16. The expression and localization of wild - type p53 - gfp fused gene on human high - metastasis hepatocellular carcinoma cell line

    熒光蛋白融合基因在高轉移人肝癌細胞中的表達與定位
  17. ( 2 ) deletions of four different lengths were conducted using pcr amplification with the information of sequence of pdf 1. 2 gene promoter. these mutated promoters were fused to the gus reporter gene and introduced into tobacco plants. the results of gus activity driven by these different constructs showed that the promoter sequence between positions - 300 and - 243 ( containing a gcc box and a g box - like sequence ) was an essential ja - responsive element region to activate the expression of gus reporter gene, and the - 300 bp position was defined as the boundary of the minimal functional promoter in response to ja signaling

    （ 2 ）對該啟動子的5 』端進行不同長度的缺失突變，突變的啟動子與gus構建的融合基因在煙草中受meja誘導的瞬時表達結果表明，該啟動子中- 300 - 243bp區域（有一個gccbox和一個gbox - likesequence ）是其應答meja處理所必須的區域，並將- 300bp作為該啟動子應答ja信號的最小區域的邊界位置。
  18. ( 3 ) on the basis of the deletion analysis, three substitution mutants ( ml : 6bp sequence upstream of gcc box m2 : gcc box and m3 : g box - like sequence ) by pcr were designed to isolate the essential ja - responsive element. transgenic tobacco plants containing promoter substitution constructs were generated by agrobacterium - rnqdiaied leaf transformation. loss - of - function experiment, using transient expression analysis of gus reporter genes, confirmed that gcc box act as an essential element to respond ja signaling in pdf1. 2 promoter

    （ 3 ）在缺失突變的基礎上，通過對gccbox及其相鄰的上下游六個堿基進行取代突變，將突變啟動子與gus構建融合基因，在煙草中受heja誘導的瞬時表達結果表明， h1和m3的突變對該啟動子應答ja信號的影響很小，而m2 （ gccbox的突變）則幾乎使該啟動子應答ja信號的功能完全喪失，所以gccbox是該啟動子中應答ja信號的必需元件。
  19. In addition, we also focused on constructing a plant virus - based gene vector pvx to express ltb - st fused gene by means of agroinfection. the possibility of producing vaccine was probed through plant virus - based gene vector expression system

    同時本研究還通過構建ltb ? st融合基因pvx病毒表達載體，並用農感染法轉化植物，探索了暫態表達系統生產疫苗的可能性。
  20. 3. using clamp technique, ex vivo gene transfer into liver graft was performed during cold preservation via perfusion of the portal vein with 5ml ringer ' s solution containing replication - defective adenovirus vector adhuctla4 - ig

    供肝冷保存時，採用血管夾技術lamptechnique ）經門靜脈灌注攜帶融合基因hllctla4dg的重組腺病毒，于術后3天、 7天能定性檢測到hllctla4ig在受體外劃血卜1 。
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