蟲體原基 的英文怎麼說
中文拼音 [chóngtǐyuánjī]
蟲體原基
英文
polypidian primordium-
Genomic analysis of parasitic human pathogens, particularly plasmodium falciparum, and leishmania major
人類寄生性病原體的基因體分析，特別是瘧原蟲與利什曼原蟲。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Research topics cover the basic and applied parasitology, including the major parasitic groups, pathogenic biology, host specificity, epidemiology, ecology, the control of parasitic infections, and biochemical and molecular parasitology, and ranging from host - parasite relationships of intrinsic biological interest to those of social and economic importance in human and veterinary medicine and agriculture
在整體和分子水平上，應用現代生物技術對危害人體和重要經濟動植物的主要寄生蟲（原蟲類、吸蟲類、絳蟲類、動物線蟲類、植物線蟲類及醫學昆蟲等）和由其引起的病蟲害進行研究，在病原生物學（包括生活史、媒介種類、基因組結構與表達調控等） 、流行病學、生態學，寄生動物與宿主間的相互作用關系等研究基礎上，實現對病原物的診斷、治療和控制。Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues
二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。The 496 bp fragment of the orf of p22 gene and a 561 bp fragment were amplified from the genomic dna of zs strains of toxoplasma gondii. both 496 bp and 561 bp fragments were successfully cloned into the plasmid pthiohisa, b, c and pbudce 4. 1 respectively. 2
從弓形蟲zs株基因組中擴增出p22編碼基因的一長496bp ，另一長561bp的片段，並成功構建含p22編碼基因的原核質粒重組體pthiohisa ， b ， c / p22 ，及真核重組表達質粒pbudce4 . 1 / p22 。The research interests of this group include : aborvirus diagnosis technology development and the interaction of aborvirus and mosquitoes, entomopathogenic bacteria and insecticidal gene resources, microbial genomics and comparative genomics, insecticidal proteins and their mode of action, construction of engineering strains with higher toxicity and wider active spectrum, production, standardization and the application of bio - pesticide and other microbial agents, resistance mechanism in target insects and the resistance management
重點研究登革熱病毒、乙型腦炎病毒和西尼羅病毒的快速檢測及病毒與宿主的相互作用關系，蚊蟲病原微生物菌種及其基因資源，微生物基因組學和比較基因組學，殺蚊毒素蛋白特性和作用方式、殺蚊細菌的遺傳改良和工程菌株的構建，新型細菌殺蚊制劑的研製及野生型和重組微生物對環境的安全性評估等，發展新的生物防治技術，建立和完善以生物防治為主的蟲媒病毒媒介蚊蟲綜合防治體系。The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male
Page電泳結果表明：麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白，分子量分別為181kd和136kd ； sds - page電泳分析：存在3條雌特異性帶，其分子量為123kd 、 120kd和91kd ，由此，可推定卵黃原蛋白（ vitellogenin ， vg ）和卵黃磷蛋白（ vitellin ， vn ）由2個蛋白組成，其中分子量較大的蛋白由2個亞基組成；雙向電泳結果顯示，在120kd附近有兩個特異性點，其等電點為5 . 5和5 . 7 ；雙擴散表明，麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應，而與雄蜂血淋巴無免疫反應，說明了vg與vn具有免疫同源性，是雌特異性蛋白，且由脂肪體合成。In this study, a new gene c / wew, encoding cholesterol oxidase, was isolated from rhodococcus equi. 4 - 2g2 found in china, which may be useful in clinical diagnosis healthy food and pest management in agriculture. in addition, the gene has been expressed successfully, the expression product has cholesterol oxidase activity, thus this work provided theoretical basis to the development of genetic engineering of cholesterol oxidase
本研究從我國自行分離的馬紅球菌4 ? 2g2菌株中分離到一種編碼膽固醇氧化酶的新基因choew ，它將可應用於臨床檢測、保健食品和農業抗蟲等領域；同時利用原核表達載體成功的在大腸桿菌中表達了目的基因，表現出膽固醇氧化酶酶活，為膽固醇氧化酶的基因工程利用開發作了一定的理論實驗基礎研究。In this study we had used the sensor to detect antibody - antigen reactions of fasciolsis, nd, ibd and eds. it is proved the sensor is fitted to use in detecting antibody - antigen reactions of these diseases. the results of detecting antibody - antigen reactions of fasciola gegatica, nd, ibd and eds by using the sensor basically conform to that with standard samples
在實驗研究中，我們先後應用電化學免疫傳感器測試了大片形吸蟲，雞新城疫（ nd ） 、雞傳染性法氏囊病（ ibd ） 、雞減蛋綜合征（ eds ）等的抗原抗體反應，結果與我們先前初步設定的判斷標準基本相符。An organ phosphorous pesticide, 4 - ( methylthio ) phenyl di - n - propyl phosphate ( propaphos ), and intermediates, di - n - propyl phosphite and di - n - propyl chlorphosphate, were synthesized by using n - propanol, phosphorus trichloride, 4 - ( methylthio ) phenol, etc. as starting materials
摘要以正丙醇、三氯化磷、 4 -甲硫基苯酚等為原料，合成有機磷殺蟲、殺菌劑丙蟲磷以及中間體二正丙基亞磷酸酯等。Some of this last third are known : genetic predisposition, ultraviolet sunlight, pollutants such as pesticides, and other factors including cosmic radiation and a naturally occurring radioactive gas called radon
已知的這些原因有：基因缺陷、日光的紫外線輻射、殺蟲劑之類的污染物，其他的原因包括宇宙射線和自然產生的放射性氣體? ?氡。Cloning and sequence analysis of the ribosomal dna its gene of leishmania donovani isolates from hill foci of china
山丘疫區杜氏利什曼原蟲核糖體基因內轉錄間隔區的克隆及序列分析Ts87 gene fragment was picked out by screening adult t. s cdna library using sera of rabbit raised against soluble antigen of t. s and using sera of rabbit infected artificially with t. s. the ts87 fragment was ligated to vector pcdnas. l, which contains a cmv promoter / enhancer
本研究採用通過免疫篩庫得到的旋毛蟲抗原基因片段ts87與載體pcdna3 . 1連接。該載體含有人類巨細胞病毒（ cmv ）的高效啟動子和增強子，是一種外源基因在哺乳動物細胞內高效表達的理想載體。分享友人