血清病抗原 的英文怎麼說
中文拼音 [xiěqīngbìngkàngyuán]
血清病抗原
英文
serum sickness antigen- 血 : 血名詞(血液 多用於口語) blood:吐血 spit (up) blood; 血的教訓 a lesson paid for [written] in b...
- 清 : Ⅰ形容詞1 (純凈) unmixed; clear 2 (寂靜) quiet 3 (清楚) distinct; clarified 4 (一點不留) w...
- 病 : Ⅰ名詞1 (疾病; 失去健康的狀態) illness; sickness; disease; malum; nosema; malady; morbus; vitium...
- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 原 : Ⅰ形容詞1 (最初的; 原來的) primary; original; former 2 (沒有加工的) unprocessed; raw Ⅱ動詞(原...
- 血清 : [免疫學] serum; blood serum血清病 serum sickness
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Scientists worry about vaccines that depend on antigens collected from the serum of carriers.
科學家們對依靠從帶病者血清中提取的抗原作為疫苗很不放心。From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本實驗採用spf雞胚及雞胚原代成纖維細胞,從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞,並且這種凝集可以被特異性抗血清所抑制。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。In group a, rabbits were immunized with isologous lens antigens ( il - ags ), + cfa while in group b rabbits were injected with pbs + cfa
方法:採用同種晶狀體抗原免疫家兔;觀察血清特異性抗體水平,晶狀體病理和裂隙燈下晶狀體形態學變化以及視覺電生理改變。Our results demonstrated that patients with pustules, erythroderma and poor control status showed higher serum levels of scc ag
結果顯示有膿皰,紅皮癥,及控制不良的乾癬病患,其血清的鱗狀細胞癌抗原會較高。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗,將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合,獲得新抗原- 35肽序列;並在合成、純化後分別與弗氏佐劑、免疫刺激復合物( iscoms )混合后免疫不同遺傳背景的雌性小鼠,觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變:以及在ivf下,新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。Study of serodiagnosis method for lyme disease using protein of flagellin of borrelia burgdorferi
重組伯氏疏螺旋體鞭毛抗原用於萊姆病血清學診斷方法的研究The diaphragm had the ability to detect the positive serum when it was diluted to 2 ' 11 and so it has good sensitivity ; stored at 4 for at least 7 months, the sensitivity and specificity of the diaphragm did not change, so it has good stability ; when 10 positive serum was detected 3 times, the result is reproducible, so the diaphragm has good reproducible. serums from experimental inoculated piglets was detected. the results showed that when the titer is l : 16, the pigs were infected with streptococcus suis ; and when 1 : 64, the pigs could survive after challege with streptococcus suis. all the results have shown that dot - ppa - elisa was a convenient, rapid, sensitive specific useful method for the detection of antibody
該法以硝酸纖維素膜為固相載體,包被膜載抗原製成的診斷膜片具有良好的特異性:不與仔豬副傷寒、豬巴氏桿菌病、豬大腸桿菌病、豬衣原體病、豬瘟、豬細小病毒病、豬偽狂犬病、豬布氏桿菌、豬丹毒陽性血清反應;膜片具有良好的靈敏性,陽性血清作2 ~ ( - 11 )稀釋亦能檢出;膜片具有良好的穩定性,在4至少能保存7個月,其靈敏性不變。Colibacollosis serotype k. 99 positive sera in ducks and duck plague positive sera were tested, whereas, the results of detection to r. a. sera were positive. the sensitivity of indirect elisa were 50 to 100 times ( serotype 1 ), ( 2 ) 25 to 100 times ( serotype 2 ), ( 3 ) 12 to 100 times ( serotype 4 ) and 25 to 200 times ( serotype 5 ) th an micro - agglutination test
包被鴨疫里默氏桿菌抗原時,對鴨抗5 : a多殺性巴氏桿菌陽性血清、鴨抗型鴨病毒性肝炎陽性血清、鴨抗鴨源大腸桿菌k _ ( 88 )陽性血清、鴨抗鴨源腸炎沙門氏菌陽性血清、鴨抗鴨源大腸桿菌k _ ( 99 )陽性血清、鴨抗鴨瘟陽性血清檢測結果呈陰性。These data suggest that scc ag levels may be an indicator of psoriasis activity
由此可知血清的鱗狀細胞癌抗原可能為乾癬疾病活動力的指標。A dot - ppa - elisa has been developed to detect the specific antibody against streptococcus suis. the antigen was isolated from group c d e r and type 2 streptococcus suis by three different method : ( a ) autoclave extraction method, ( b ) hcl - extraction method, and ( c ) fuller ' s method
本研究選取c 、 d 、 e 、 r群及2型鏈球菌標準菌株,以高壓法提取抗原建立了檢測豬鏈球菌病血清抗體的dot - ppa - elisa方法。The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively
豬瘟病毒ez基因的原核表達: pcr擴增出當前豬瘟流行野毒株,中國豬瘟兔化弱毒( c株)兔脾組織毒ez基因的主要抗原區,將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達,經sds page檢測表明,重組質粒能表達ez基因主要區蛋白, westernblot檢測表明,誘導表達蛋白與豬瘟陽性血清發生特異性反應,表達量為35和38 ,可用於基因工程診斷抗原。A dot - ppa - elisa method has been developed to detect the antibody in swine serum against pasteurellosis. the capsule polysaccharide and outer membrane protein of pasteurella multocida was prepared as the diaphragm antigen
本研究提取豬巴氏桿菌菌株的莢膜和外膜蛋白抗原,首次建立了檢測豬巴氏桿菌病血清抗體的dot - ppa - elisa方法。The expression of recombinant infectious bursal disease ( ibdv ) vp2 gene in transgenic tobacco was studied. it revealed that the vp2 produced in transgenic tobacco could react with serum specific for ibdv. these results were the basic of investigating the immunological function of foreign protein vp2 produced in transgenic plants and bolstered the concept of using transgenic plants for a novel, edible, safe vaccine production
研究了傳染性法氏囊病病毒抗原蛋白基因ibdvvp2在煙草中的表達情況,證明轉基因煙草中產生了能與ibdv特異抗血清反應的ibdv抗原蛋白,為研究轉基因植物中生產的外源蛋白vp2的免疫學功能奠定了基礎,並為利用轉基因植物生產可食疫苗提供了實驗依據。Of 5 immunogens. groel and ef were reported in other pathogens
其中groel與ef在其他病原菌的抗血清中也存在抗體。Circulating antigen detection in schistosomiasis japonica patients by sandwich elisa using polyclonal and monoclonal antibodies
法檢測日本血吸蟲病患者血清循環抗原Methods a double mono - clonal antibody sandwich enzyme - linked immunosorbent assay ( elisa ) was used to detect the circulating antigens, antibodies and immune complex in 109 sera of neurocysticercosis
方法:用elisa法檢測109例腦囊尾蚴病患者血清抗原、抗體及循環免疫復合物。This paper describes the development of a sensitive, serogroup - specific elisa for detecting antibodies to mouse parvovirus and distinguishing them from antibodies to minute virus of mice
本篇描述一個偵測小鼠小病毒抗原具敏感性且血清群特異之elisa ,並且可以與其他微細小鼠病毒之抗體區隔。In immuno - blotting, these fragments reacted specifically with hepatitis c patients " sera, suggesting that e. coli - derived e2 proteins carried hcv e2 - specific, glycosylation - and - conformation - independent epitopes
在免疫印跡檢測中,上述片段與丙型肝炎病人血清有特異性反應,表明大腸桿菌系統表達的e2蛋白攜帶有hcve2特異的、不依賴于糖化和立體構象的抗原決定簇。Analysis of h9 subtype : ( 1 ) seven out of fourteen h9 strains tested shared similar reaction patterns with dk / hk / y280 / 97 or ck / hk / g9 / 97 virus, while the rest showed almost no inhibition and low reactivity to all reference antisera
Hg亞型的分析: ( 1 )抗原性分析表明七株hgnz病毒與w hk yzso 97和n hk gg 97病毒反應相似。而其它病毒株與抗血清不反應或反應的滴度很低。分享友人