血胚細胞的 的英文怎麼說
中文拼音 [xiěpēixìbāode]
血胚細胞的
英文
hemocytoblastic- 血 : 血名詞(血液 多用於口語) blood:吐血 spit (up) blood; 血的教訓 a lesson paid for [written] in b...
- 胚 : 名詞[生物學] (初期發育的生物體) embryo
- 細 : 形容詞1 (條狀物橫剖面小) thin; slender 2 (顆粒小) in small particles; fine 3 (音量小) thin ...
- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 的 : 4次方是 The fourth power of 2 is direction
- 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
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From dead chickens, one virrus was isolated by using eggs and chicken embryo fibroblast. lt was able to agglutinate chicken ' s erythrocytes and this heamagglutination could be inhibited by newcastle disease antiserum. this strain ' s biological property was tested by barren spot, cross - enutralization and cross - heamagglution inhibited and it was found that it was homological with the standard newcastle disease virus ( ndv ) virulent strain and avirulent strain but it had some diference with the standard strain
本實驗採用spf雞胚及雞胚原代成纖維細胞,從河北省某雞場新城疫免疫抗體很高的病死雞的腦組織中分離得到一株病毒。此株病毒能凝集雞的紅細胞,並且這種凝集可以被特異性抗血清所抑制。The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以細胞培養液m199 (含2既的小牛血清,常規量雙抗)為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式(例如,從0一一6 ,凍存速度為一0 . 05 / min ,再以一0 . 5 / min的速度從一6一一40 ) ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養,解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁,但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52
以細胞培養液m199 (含20 %的小牛血清)為稀釋液, dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣,採取先慢后快的方式,冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍,成活率為72 % ,第二組冷凍保存13天後解凍,成活率為520 / 0 。Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f )的es細胞培養液中形成典型的胚胎幹細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。As a member of this superfamily, human acidic fibroblast growth factor ( afgf or fgf1 ), expressed by a variety of cells from all three germ layers, shows great potential for clinical applications for therapy of a variety of diseases, such as parkinson ' s disease, spinal cord contusion injury, neural regeneration in reimplantation of broken - off finger, brain ischemia, renal ischemia, myocardial infarction, occlusive vascularitis, retinal ischemia, gastric ulcer and nonhealing wound and so on
人類酸性成纖維細胞生長因子( afgf , fgf - 1 )是成纖維細胞生長因子超家族成員。來自於三個胚層的多種細胞都可以表達afgf 。 afgf在治療帕金森綜合癥、急性脊柱扭曲性損傷、斷指中神經功能重建、腦缺血、腎缺血、心肌梗塞、閉塞性脈管炎、視網膜缺血、胃潰瘍及難愈合性傷口等多種臨床應用方面具有巨大潛力。Development of chicken embryo fibroblast cell line resistant to j subgroup avian leukosis virus infection
亞群禽白血病病毒的雞胚成纖維細胞系建立( 3 ) isolation and culture of human primordial germ cells ( pgcs ). human pgcs collected from gonadal ridges and mesenteries were grown on mouse feeder layers in the presence of human recombinant leukemia inhibitory factor ( lif ), human recombinant basic fibroblast growth factor, and forskolin as described previously. initially, pgcs were visualized by alkaline phosphatase activity staining
( 3 )人類pgcs的分離和培養從4 10周齡藥物流產胚胎的生殖嵴和腸系膜組織中分離原始生殖細胞( primordialgermcells , pgcs ) ,培養在添加人重組白血病抑制因子( lif ) 、人重組堿性成纖維細胞生長因子( bfgf )和forskolin的小鼠飼養層細胞上。4 ) after the activated nuclear transfer embryos had been cultured in m199 ^ and czbm for 48h, the cleavage rate can reach at 16. 41 % and 17. 41 %, and the morula rate were 7. 46 %, % and 6. 74 %, respectively
08融合率:血請饑餓法和nocodazole法處理的供體細胞構建的重組胚胎,經6 dmap乙醇ccb激活,分別獲得17 33o和17 02o的卵裂率以及6The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days. the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying. the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage. the chicken embryo dead in a week after it inoculated. the body was dropsy and hemorrhage. dead before it hatched out, the embyo body were dropsy, pale and slime. the liver was yellow and swolled, gallbladder ( vesica fellea ) was filled with bile. bursa and glandula thymus analosis. the kindey dropsy. bowel lamina were humble, dilatation. gas and yellow foam were filled the bowel. histopathological changes were that, in early stage, obvious changes of liver and kindey were dropsy, hemorrhage and necrosis. two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs. the obvious changes of bursa were dropsy, adverse folliiculated growth and little lymphocytes proliferating, 19 - 21 days chicken embryo, one or two big empty vacuoles were prensent in cells of liver and kindey. the number of the folliculi was growing, the vacuoles between cells were larger
膽囊充盈、其內充滿稀薄的膽汁;法氏囊、胸腺萎縮,腸道擴張、腸壁菲薄、內充滿氣體及黃色泡沫狀物;腎臟腫大。病理組織學變化方面,早期肝臟、腎臟、腸主要以出血、水腫和壞死為主,且肝細胞核及腎小管的上皮細胞核內均發現有核內包涵體,包涵體呈嗜酸性,為大型圓形包涵體或不規則的顆粒狀;法氏囊則以水腫、濾泡發育不良、小型淋巴細胞數量增多為主。 19 21日齡雞胚肝細胞、腎小管上皮細胞的胞漿內出現1 2各大的空泡,法氏囊濾泡數目增多細胞間有較大空隙。The endocrine cells in the digestive and glands of alligator sinensis embryos aged from 8th to 55th day were localized and compared by using immunohistochemical method with thirteen kinds of antiseras of hormone. during the development of pancreas in alligator sinensis embryos, somatostatin ( ss ) immunoreactive ( ir ) cells, 5 - hydroxytryptamine ( 5 - ht ) - ir cells, glucagon ( glu ) - ir cells, epidermal growth factor ( egf ) - ir cells appeared on 18th day. no p53 protein - ir cell, gastrin - ir cell, testosterone - ir cell, chromogranin a - ir cell, vasoactive intestinal polypeptide - ir cell, epithelial membrane antigen - ir cell or insulin - ir cell was found in the pancreas of alligator sinensis embryos
本實驗採用免疫組織化學技術,應用13種不同的抗血清,對孵育時間8 ? 55天揚子鱷胚胎消化道及消化腺內分泌細胞的種類進行鑒別、定位和比較,結果如下:揚子鱷胚胎胰腺中,生長抑素、 5 ?羥色胺、胰高血糖素、表皮生長因子、胰多肽免疫反應陽性細胞出現于第8天; p物質免疫陽性細胞出現于第18天; p53 、胃泌素、睪酮、嗜鉻素a 、血管活性腸肽、上皮膜骯原、胰島素免疫陽性細胞在各期揚子鱷胚胎胰腺中均未發現。Granulocytic sarcoma ( gs ), also known as extramedullary myeloblastoma or chloroma is an unusual manifestation and represent soft tissue masses composed of immature cells of the granulocytic series
顆粒性白血球肉瘤也稱為骨髓胚細胞瘤或綠色瘤,是一種少見的疾病。Pre - clinical studies showed that needleless injected fluids follow the path of least resistance and does not pass into the substance of bone, media of blood vessels or into the fibre substance of the nerve trunks
臨床研究顯示,無針美槊療法所注入的液體不會滲入骨頭、血管或神經內,它會沿著最少抗體的路徑進入肌膚組織,並且加速皮膚細胞的新陳代謝,讓雞尾酒中胚層綜合物質更容易於肌膚所吸收。In addition, the stomach contained glu - ir cellsn gas - ir cells and vip - ir cells, and the duodenum contained glu - ir cells and gas - ir cells. different endocrine cells varied from one to another in the distribution pattern, the stage of appearance and the number. no glu - ir cell, gas - ir cell or vip - ir cell was found in the oesophagus and no vip - ir cell in the duodenum
另外,胚胎期揚子鱷消化道內分泌細胞的種類和分佈與成體有差異,胚胎期消化道檢出胰高血糖素、胰多肽和p物質免疫陽性細胞,而成體未檢出;胚胎期消化道中胃泌素、血管活性腸肽,生長抑素免疫陽性細胞分佈與成體消化道不同。The morphology of single cell and the ultrastructure of cell membrane were observed. by means of afm, the ultra - thin sections of murine es cells were investigated in order to make afm capable of gaining the information of the inner structure of cells. in addition, the morphological changes and damaging effect of trichosanthin ( tcs ) on red blood cell ( rbc ) membrane were observed by afm
對原子力顯微鏡( atomicforcemicroscope , afm )的成像技術進行了多方面探索;用afm研究膠原蛋白分子在雲母表面的吸附和自組裝行為;對小鼠胚胎幹細胞和人血紅細胞進行afm成像,觀測單個細胞的形態以及細胞膜的微觀結構;利用afm得到了小鼠胚胎幹細胞超薄切片的高解析度圖像,探索用afm研究細胞內部結構,拓展其應用領域;天花粉蛋白( tcs )和紅細胞的相互作用,利用afm觀察到天花粉蛋白( tcs )和紅細胞相互作用前後紅細胞膜超微結構的變化,據此討論了二者的作用機理。The results of the experiments showed that hydrolysates can remarkably enhance the thymus and spleen index, the phagocytsis index and phagocytsis percent of macrophage, and the lymphocyte transformation for normal mouse while also promote the form of hemolysin in mouse body
研究結果表明,玉米胚芽蛋白酶解物能顯著提高正常小鼠的免疫臟器指數、腹腔巨噬細胞的吞噬百分率、吞噬指數和淋巴細胞的轉化功能活性,促進溶血素的形成。Promotive action of lysophosphatidic acid on proliferation of rat embryonic neural stem cells and their differentiation to cholinergic neurons in vitro
溶血磷脂酸促進離體大鼠胚胎神經幹細胞的增殖及其向膽堿能神經元分化Experimental condition optimization study on inducing mouse embryonic stem cells differentiation into insulin producing cells by serum - free culture in vitro
無血清培養法誘導小鼠胚胎幹細胞分化為胰島素分泌細胞的實驗條件優化選擇Finding regulatory pathways specific to fetal blood - forming cells could help scientists understand childhood leukemias and generate blood - forming cells for bone marrow transplants, said the researchers
研究者提出找到胚胎造血幹細胞特殊的調控路徑可以幫助科學家理解兒童白血病,以及讓骨髓移植體產生造血幹細胞。In a discovery that fills a critical gap in the understanding of stem cells, researchers have discovered a protein that fetal, but not adult, blood - forming stem cells need to replenish themselves
在幹細胞的理解上,一個發現填充了一個重要的缺口,研究人員已經發現一種蛋白質- -胚胎(而非成人)的造血幹細胞,需要自我補充。In contrast to murine studies, studies of human hscs have relied on a number of in vitro assays that identify cells with the capacity to generate hematopoietic progenitors in long - term culture and to differentiation into multiple hematopoietic lineages. recently, two elegant animal models that enable the in vivo measurement of human hscs marrow repopulating potential have been developed. one is the sheep in utero transplantation system in which human hematopoietic progenitor cells are transferred into the sheep fetus in utero before the development of the ovine immune system
早在80年代中期, zanjani等就利用胚胎期免疫系統發育不完善,將人的造血幹細胞移植到妊娠中期胚羊腹腔內,成功地建立了人胎羊造血幹細胞嵌合體移植模型,並以此實驗模型作為體內檢測人造血幹細胞和人骨髓間充質幹細胞多向分化潛能的方法。分享友人