裂合酶 的英文怎麼說

中文拼音 [liègě]
裂合酶 英文
lyase
  • 裂 : 裂動詞[方言] （東西的兩部分向兩旁分開） open
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  1. As the cyclin dependent kinase, cdc2 may act at multiple levels during mitosis to repress ribosome biogenesis, which lead the biosynthesis to a relative silent phase, when the most of cell ' s energy expenditure is used in chromosome condensation, breakdown of the nuclear envelope, and formation of the mitotic spindle

    作為cyclin依賴激酶， cdc2的激酶活性在很大程度上抑制了細胞的合成代謝，使細胞的蛋白合成進入了一個相對的靜止期，而細胞主要的能量則被應用於細胞分裂時的染色體的濃縮、核膜的降解、紡錘體的形成等。
  2. Many organisms produce enzymes, termed photolyases, that specifically bind to these damage products and split the via a uv - a / blue light - dependent mechanism ( photoreactivation ), thereby reversing the damage. these two photolyase are specific for either cpds ( cpd photolyase ) or 6 - 4 products ( 6 - 4 photolyase ). a gene that expresses a protein with 6 - 4 photolyase activity in vitro, was recently cloned from high organisms ( arabidopsis thaliam, drosophila melanogaster, danio rerio, xenopus laevis and homo sapiens )

    目前已從高等生物擬南芥、鮐類、果蠅、人類和非洲爪蟾蜍屬中克隆到有（ 6 - 4 ）光裂合酶活性的基因，本研究從鹽生杜氏藻dunaliellasalina中克隆到（ 6 - 4 ）光裂合酶的基因，並將該基因在大腸桿菌中得以表達，這是首次在藻類中克隆到（ 6 - 4 ）光裂合酶基因，對光裂合酶的研究具有重要意義。
  3. We report here the cloning of a homolog of this gene from dunaliella salina. this cloned gene produces as protein with photolyase activity when expressd in escherichia coli

    運用blast方法對est序列進行分析，初步預測該est序列為光裂合酶藍光受體蛋白質家族中一個成員的部分cdna 。
  4. 5. after induced with iptg, jm109 with pgex - 4t - l / 6 - 4 was radiated by uv for 30 seconds, cultivate these e. coli with light or without light. the survival rate proved the gene of d. salina ( 6 - 4 ) photolyase has photoreactivation

    5 .工ptg誘導過的轉化菌，經過30秒的紫外光照射后，分別進行光培養或暗培養，計算存活率，從而證明了及sa了z 』 na的（ 6一4 ）光裂合酶在大腸桿菌中可進行光修復活性作用。
  5. Introduction telomerase is a ribonucleoprotein reverse transcriptase that synthesizes telo - meric dna sequences using its rna as template. it can remedy the loss of te - lomere after cellular mitosis, maintain telomere length and stabilize chromosome

    前言端粒酶（ telomerase ）是一種核糖核蛋白逆轉錄酶，能以自身的rna為模板，從頭合成染色體末端的端粒dna ，彌補細胞分裂時端粒dna的丟失，維持端粒的長度並穩定染色體。
  6. ( 3 ) uv - b radiation increased the phenylalanina ammonia - lyase ( pal ) activity in leafy thallus of duckweeds, and as a consequence, flavonoids were accumulated. flavonoids synthesis induced by supplementary uv - b is an adaptive response of duckweeks under enhanced uv - b radition

    （ 3 ）紫萍可以通過提高uv - b吸收物質如類黃酮合成酶苯丙氨酸氨裂解酶（ pal ）活性，促進類黃酮的積累來適應uv - b輻射的脅迫。
  7. The cyst cells enclosing spermatomeres maybe synthesize a kind of scf - like protein, which can recognize specially the c - kit receptor on the cellular membrane of spermatomeres. then c - kit is activated, dimerizing and autophosphorylating. at the same time, the tyrosine kinase domain of c - kit is activized, which phosphorylates the proteins that have sh2 domain

    精母細胞周圍的囊細胞可能合成scf樣蛋白，特異地識別精母細胞膜上的c - kit受體，並刺激c - kit發生二聚化、自體磷酸化，激活胞內酪氨酸激酶活性，活化具有「 sh _ 2結構域」的靶蛋白，可能通過一系列信號級聯，最終激活與減數分裂的相關蛋白或基因。
  8. Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel

    融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。
  9. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  10. Telomerase is at end of the telomere, it helps to keep the sequence of dna, to offset or postpone the continuously shortening of the telomere while the cell division takes place

    端粒酶位於端粒末端，作用是合成端粒dna序列，以抵消或延緩端粒隨細胞分裂的不斷縮短。
  11. Hydroperoxides were produced from unsaturated fatty acid in oils catalyzed by lipoxygenase, then in the hydroperoxide lyase, the grassy green and leafy green aroma volatile aldehydes were made

    摘要脂肪氧合酶催化油脂中的不飽和脂肪酸生成氫過氧化物，氫過氧化物再在裂解酶的作用下，生成具有青草香、青葉香香氣特徵的揮發性醛類物質。
  12. The basic approach of unsaturated fatty acid catalyzed and oxygened by enzyme, the preparation of lipoxygenase and production of hydroperoxides, the preparation of hydroperoxide lyase and cleaving of hydroperoxides were mostly discussed

    主要論述了不飽和脂肪酸酶催化氧化的基本途徑，脂肪氧合酶的制備及氫過氧化物的生成，裂解酶的制備及氫過氧化物的裂解過程。
  13. Then, nap300 and 75 were fused in a similar way to phba gene encoding 3 - ketothiolase for a direct comparison. the result indicated that the two promoter ' s expression efficiency reached a peak at the same developmental stage of tobacco, which means they have the advantage of being used simultaneously for expressing different foreign genes in plant

    將nap300 、 7s分別與phba基因（編碼3 -酮硫裂解酶）相連，在相似表達環境中對二者功能進行比較，發現兩個啟動子表達模式基本相同並在同一時期達到活性高峰，因此nap300可用於改善phb合成基因在植物體內的表達調控。
  14. It " s the first cdna code ( 6 - 4 ) photolyase found in low orgnism ( alga ), and this study is important for the reseach of 6 - 4 photolyase. the methods and analysis as bellows : 1. this est was analyzed by method of blast, and the conclusion suggests that the sequence probably be a partial cdna that can code one of protein family of photolyase / blue light photoreceptor

    利用3 』 race法擴增此cdna序列的3 』端部分，所得片段長度為2575bp ，含一個完整的開放讀框，長1800bp ，可編碼600個氨基酸；利用生物信息學法對此擴增序列進行分析，包括同源性分析、序列的讀框分析、蛋白質的保守性分析以及該蛋白的進化關系分析，預測出此序列編碼了d . salina的（ 6 - 4 ）光裂合酶。
  15. Numerous hairy roots were induced from protocalli on ms medium without any growth regulator. the paper electrophoresis revealed that all of the regenerated hairy roots tested synthesized the corresponding opines

    原生質體分裂形成的愈傷組織在無激素ms培養基上再分化出的發狀根仍具冠癭堿合成酶活性。
  16. Methods : extracting the total rna of human pbmc, the objective include 60 healthy blood donator, 30 patient with viral encephalitis and multiple sclerosis and parkinsonian syndrome, 30 patient with schizophrenia and affective disorder, this indviuals were inpatients or outpatients of the first hospital of chongqing university of medical science from december, 2000 to june, 2001. using nested rt - pcr techique to detect borna disease virus " middle fragment in orf i, and using southern blot hybridization to analyze the pcr product

    重慶醫科大學碩士學位論文方法：提取從2000年12月至2001年6月在重慶醫科大學第一附屬醫院神經科和精神科住院及門診的60例健康獻血者、 30例包括原因未明的病毒性腦炎、多發性硬化、帕金森綜合征，以及30例包括精神分裂癥和情感障礙患者pbmc中的總kn 』 a ，採用套式逆轉錄聚合酶鏈反應estedrticr ）技術進行了bdvorf基因中部片段的檢測，並對pcr產物進行電泳分析及southernblot雜交分析。
  17. 4. the expressed result of pbv - a, pbv - b and pbv - c showed that the expressed protein was soluble and no inclusion body was been found. sds - page analysis show the molecular weight of protein expressed by phaa was 42kda which was equal to 3 - ketohilase, the molecular weight of protein expressed by phab was 26kda which was equal to acetoacetyl - coa reductase, and the molecular weight of protein expressed by phac was 63kda which was equal to pha synthase

    表達載體pbv - a 、 pbv - b和pbv - c經誘導表達，發現所表達的蛋白均為可溶性蛋白，沒有包涵體出現；蛋白經sds ? page分析表明，基因phaa表達的蛋白分子量為42kda ，與?酮硫裂解酶分子量大小一致；基因phab表達的蛋白分子量為26kda ，與乙酰乙酰coa還原酶分子量大小一致；基因phac表達的蛋白分子量為63kda ，與pha合成酶分子量大小一致。
  18. Protein degradation is one of the major regulatory processes that allow the adaptation, repair, or removal of mutated or damaged thylakoid proteins during environmental or developmental changes. intracellular proteases play an essential role in the turnover of intracellular proteins

    在光合作用中，葉綠體類囊體膜蛋白復合物進行光能的吸收、傳遞和轉化，水的裂解及光合磷酸化等，這些膜蛋白的周轉更新需要蛋白酶的作用。
  19. A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses

    依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。
  20. We find that the fusion proteins are inclusion bodies when the bacteria lysised by the lysozyme. the product of pet - 32a ( + ) - igf - i was analyzed by western blotting, which confirmed that the hybrid protein was expressed as expected

    菌體採用溶菌酶裂解，融合蛋白以包涵體形式存在；對higf -融合蛋白進行了westernblot分析，確認所表達蛋白為目的蛋白。
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