親核物質 的英文怎麼說
中文拼音 [qīnhéwùzhí]
親核物質
英文
nucleophile-
Unicolor and lilium asiatic hybrids or cultivars in lilium asiatic hybrids were researched with their parents by karyotype, soluble protein, esterase isoenzyme and peroxidase isoenzyme. the results provided identification markers of cytology and biochemistry for hybridization at the early stage in lily breeding programs. the cluster analysis according to similarity coefficient of soluble protein and peroxidase showed that " yellow " and " omega " have the most closest relation in lilium asiatic hybrids
本研究對百合屬幾個植物的親緣關系進行了可溶性蛋白質、過氧化物酶同工酶分析，同時對亞洲百合雜交系內雜交及其與原種系間的雜交後代進行了核型、可溶性蛋白質、過氧化物酶和酯酶同工酶分析，以期為百合屬植物親緣關系分析提供生化依據，以及為雜交後代的早期鑒定提供細胞學、生化水平的檢測指標。To detect whether crosslinking reaction influence the fluorescence properties of the samples, oxygen - evolving psil core complexes were treated with five crosslinking agents at different concentration, then the chlorophyll fluorescence emission, excitation, and intrinsic fluorescence spectra of these samples were measured. the results showed that both chlorophyll fluorescence and intrinsic fluorescence of oxygen - evolving psil core complexes were altered by the crosslinking reaction. the maximum chlorophyll fluorescence band at 682 nm decreased and the intrinsic fluorescence emission intensity at 308 nm and 328 nm either increased or decreased
結果表明：交聯處理對psll放氧核心復合物葉綠素熒光和內源熒光都有影響，引起682urn處葉綠素熒光強度的降低、 308urn或328urn處蛋白質內源熒光強度的增大或減小，並與處理時所用交聯劑的濃度、交聯劑的親疏水性和交聯臂長相關。When copper exceeds the cellular needs, it is toxic through the production of highly reactive hydroxyl radicals, that have deleterious effects on cellular components including destabilization of plasma membranes and lysosomal membranes
但是也具有毒性，銅可以與一些脂質，蛋白質和核酸等物質高親和性地結合，並催化其氧化，所以維持銅濃度在一定的正常范圍很重要。Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3. the recombinant plasmid was transformed to e. coli bl21 and the gst / mpem fusion protein was induced to express with iptg. the fusion protein was purified by affinity chromatography
方法： pcr擴增小鼠pemcdna編碼序列，將它克隆到含有gst的原核表達質粒載體pgex - 4t - 3上，轉化大腸桿菌bl21 ，誘導表達gst mpem融合蛋白，通過親和層析，獲得初步純化的產物，以羥胺切割驗證其一級結構。分享友人