質粒轉導 的英文怎麼說

中文拼音 [zhílìzhuǎndǎo]
質粒轉導 英文
plasmid transduction
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 轉 : 轉構詞成分。
  • 導 : 動詞1. （引導） lead; guide 2. （傳導） transmit; conduct 3. （開導） instruct; teach; give guidance to
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )

    獲得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育，我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發芽的葉片，共207片（基因槍109片，農桿菌98片） 。在含basta0 . 4mg l的篩選培養基上進行篩選，得到抗性葉片181片（基因槍93片，農桿菌88片） 。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. This paper describes a strategy that has developed to transfer the cdna of tobacco mnsod gene into the commercially important breeding line - baoding alfalfa via agrobacterium infection. transgenic alfalfa plants have been generated that overproduce a nicotiana plumbaginifolia l. manganese superoxide dismutase ( mnsod ). the results domenstrated that baoding alfalfa is an important breeding line which easily amenable to genetic transformation

    本研究採用我國農藝性狀優良的豐產苜蓿品種保定苜蓿，通過農桿菌介導的轉基因方法，使用特定的質粒載體pchlsod將煙草mnsod基因的cdna序列導入保定苜蓿中，說明保定苜蓿是一種易於遺傳轉化的優良苜蓿育種品系。
  5. Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector

    本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。
  6. ( 4 ) transformation of mdmv - cp gene mdmv - cp gene was successfully into r18. w18 and 501 in 2001 fall, each material being bombed 20, 15, and 10 plates. 213 bastar ( effective constitution ppt ) resistant plants were obtained of which r18, w18 and 501 resistant plants counted 103, 90, and 20 plants respectively. molecular test was undergoing

    O ） mdmvcp因的邀傳轉化於2001年秋用基因槍將mdmvcp基因巾35scpbar質粒）導入、 w18 、 501三個優良自交系，利用已建立好的遺傳轉化體系，經抗性篩選、分化、壯苗等步驟，已得到抗bastar （有效成分ppt ）植株213棵，其中r18103株、 w1890株、 50120株。
  7. Gfral was high expressed in e. coli after iptg induction. by ni2 + chelation affinity chromatography, the purified recombinant gfral protein were obtained. based on the evolutionary trace method, multiple sequence alignment and dendrogram construction were then carried out by the clustalx program and constructed a phylogenetic tree from a multiple sequence alignment for a protein family

    2 . gdnf的表達及其對pc12基因工程細胞的影響用本教研室已構建好的表達質粒pet一gdnf轉化大腸桿菌bl21 （ de3 ） ，經lmmipto誘導gdnf表達，並在niz氣nta柱上進行純化，稀釋復性后，純度達90 %以上。
  8. In the presented study, using h7721 human hepatocarcinoma cell line transfected with sense or antisense cdna of gnt - v, the effects of gnt - v on signal transduction an d its mechanism as well as the alteration of gene expression were investigated. we expected to elucidate whether and how the transfected glycosyltransferase modulate the cell signal transduction and gene expression

    本文以穩定轉染gnt - v正義或反義cdna質粒的h7721人肝癌細胞為材料，從下列四個方面對gnt - v影響信號轉導及其機制以及引起基因表達的變化進行了研究，企圖闡明糖基轉移酶轉染是怎樣調節細胞信號轉導的
  9. The recombination vectors were transformed into host strain of bl21 and induced with iptg. all the recombinant protein was expressed into inclusion body. the recombinant protein was identified with sds - page and westen - blot and purified through elution method. the muticlone antibody was got by immuning the rabbit with the purified protein

    把重組質粒轉化入表達受體菌bl21 ，經iptg誘導后都獲得了表達，且都以包涵體的表達形式存在，用sds - page和western - blot的方法對重組蛋白進行了鑒定。
  10. The suitable concentration of hyg to screen for resistant shoots was 50 mg / l. the kind of antibiotics to inhibit the growth of agrobacterium eha105 was cef, of which concentration was 250 mg / l

    以洋桔梗葉盤為外植體，通過根瘤農桿菌菌株eha105介導，用構建於質粒pcambia1301的npr1基因轉化洋桔梗。
  11. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。
  12. The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin

    本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。
  13. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  14. Reversal of hydroxycamptothecin resistant phenotype in human colon cancer cells by sirna transfection

    表達質粒轉導逆轉人結腸癌羥基喜樹堿耐藥表型的研究
  15. With the precondition that invitro tissue could survive after implantation, the study on transferring gus gene into arabidopsis callus was done. and we got the transformants. transformation efficiency was up to 2 %

    在活體組織注入后可以存活的前提下，通過對離子束介導質粒dna導入擬南芥愈傷組織的遺傳體系的研究，得到了gus基因轉入擬南芥愈傷組織的轉化植株，轉化效率達2 。
  16. The recombinant plasmid was translated into e. coli dh5 and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 22ku protein which was equal to chicken ifn - y protein in molecular weight was expressed in e. coli dh5

    將重組質粒轉化大腸桿菌dh5 ，於37誘導培養8h ， sds - page凝膠電泳表明該基因在大腸桿菌中獲得了高水平表達，表達的雞ifn -融合蛋白分子量約為22ku 。
  17. In order to establish the genetic transformation system of saussurea medusa maxism by agrobacterium rhizogenes, some work were done. the main results were following : 1 establishment of regeneration systems two systems of regeneration from saussurea medusa maxim were established without cold treatment. the somatic embryos were induced from callus cultured in mise for 35 days. the shoots were induced from cotyledon after cultured in misc 20 days, and from leave which were cultured in misl. the experiment showed that the carbon and glycine in the medium could help to increase the regeneration rate to 95 %

    為篩選水母雪蓮代謝突變體和轉基因研究奠定了一定的基礎，陳亞瓊： m質粒介導水母雪蓮的遺傳轉化及毛狀根中決刪合成的調節p義摘要也為研究類黃酮代謝途徑的關鍵酶基因的轉化、高效表達及作用機理提供了理想的實驗體系。主要的實驗結果如下： 1水母雪蓮高頻再生體系的建立通過體細胞胚胎發生途徑和器官發生途徑，水母雪蓮（ squssureamedusamaxim ）可以在常溫下獲得再生植株。
  18. The gene recombinant strain no. 42 ca n ' t generate ampramycin, which indicated that the cloned gene is involved in apramycin biosynthesis in s. tenebrarius

    通過接合轉移的方法將質粒pzxb014導入黑暗鏈黴菌h6中，篩選基因發生重組的菌株。
  19. Procaryotic expression vector recombinant was transformed into competent bl21, induced and expressed by iptg revulsant, explored the best inducing time and the best concentration of revulsant

    將原核表達載體的重組質粒轉化大腸桿菌bl21 （ de3 ） ，用iptg誘導物進行誘導表達，探索最佳誘導時間和誘導物的使用濃度。
  20. These results are very important for us to further understand the genetic background, biological characteristics, evolutionary rule and the anti - schistosomajaponicum mechanism of microtus fortis at the molecular levels. the specific base changes of the dna fragme nt between the two subspecies are probably correlative with the animal immigration, survival conditions, and species evolution. the cdna library of microtus fortis bone marrow cells was transferred in situ to nylon membrane, which was divided into eight equals ( ga ~ - gh )

    利用已經建立的東方田鼠骨髓細胞質粒cdna文庫，將cdna文庫轉化菌落印跡至尼龍膜，將膜均分成8份（ ga gh ） ，制備基因池，分別培養、提取基因池質粒dna ，通過lipofect - 2000脂質體轉染技術，將基因池質粒dna導入hek293細胞， 48h后收集轉染細胞上清液，即條件培養基。
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