轉甲基醇 的英文怎麼說
中文拼音 [zhuǎnjiǎjīchún]
轉甲基醇
英文
methylferase-
It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation
通過電轉化作用該基因片段被成功地整合至酵母cs115的染色體上，經過甲醇誘導之後，該基因得到了分泌表達。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。Methanol fuel cell is a new type of energy conversion equipment based on proton exchange membrane fuel cell. it has been received widespread attention because it directly use methanol as fuel in fuel cell. at the same time, it has wide application in the future
甲醇燃料電池是在質子交換膜燃料電池的基礎上發展起來的一種新型能量轉換裝置，由於直接將甲醇作為燃料電池的燃料，因而受到廣泛的關注，具有廣闊的應用前景，但是目前甲醇燃料電池的催化劑低的活性以及成本較高等問題限制了甲醇燃料電池的應用。The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask. having been induced with methanol for 36 hours, the target enzyme could be examined in the supernatant by measuring amidolytic activity
首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中，經電激轉化至畢氏酵母菌株gs15中，再經甲醇誘導，在150ml搖瓶畔1獲得細胞外分泌表達產物。Abstract : aim to synthesize a new prodrug, resveratrol trinicotinate. methods in presence of lithium and a catalytic amount of naphthalene, the reaction of p - methoxybenzyl trimethylsilyl ether and 3, 5 - dimethoxylbenzaldehyde gave resveratrol after a series of translation. resveratrol trinicotinate was obtained by the reaction of resveratrol and nicotinoyl chloride hydrochloride. results a mutual prodrug resveratrol trinicotinate was designed and synthesized. conclusion a novel method for synthesis of resveratrol and resveratrol trinicotinate has been afforded. the e - isomer is selectivily obtained by dehydration of the compound 2 with khso4
文摘:目的合成一種前藥白藜蘆醇煙酸酯.方法在金屬鋰片和催化量的萘的存在下， 3 ， 5 -二甲氧基苯甲醛與對甲氧基苯甲醇的三甲基硅醚反應經過一系列轉變得到白藜蘆醇，白藜蘆醇與煙酰氯反應得到白藜蘆醇煙酸酯.結果設計併合成了白藜蘆醇煙酸酯.結論提供了一種合成白藜蘆醇及白藜蘆醇煙酸酯的方法，採用khso4脫水可選擇性的得到反式產物Tecbios biodiesel process is based upon transesterification of vegetable oils and animal fat with methanol or ethanol in excess, in the presence of naoh or koh as catalyst
Tecbio公司的生物柴油工藝，主要是使用naoh或koh作為催化劑，對菜油和動物脂肪進行酯基轉移反應，制取甲醇或乙醇。The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase. main results of this research are as follow : 1, xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser. 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418. they all grew quickly on both md plates and mm plates, which proved their phenotypes of methanol utilization were mut +
主要實驗結查如下：西南農業大學碩士學位論文1 、用乃ai酶切攜帶植酸酶基因表達片段的重組質粒ppicgk夕句） a ，回收dna ，用genepulser電擊轉化畢赤酵母，塗布md平板，又用含不同濃度g418的ypd平板進行抗性篩選，得到98個可檢測到植酸酶活力的陽性轉化子，它們在md 、 mm平板上均表現快速生長，說明其甲醇利用表型是mut干。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。分享友人