轉白劑 的英文怎麼說
中文拼音 [zhuǎnbáijì]
轉白劑
英文
leucotrope-
In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation
本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants
血吸蟲病疫苗研究已有60多年的歷史，大致經歷了死疫苗、活疫苗（照射致弱尾蚴疫苗）和基因工程疫苗等研究階段，產生了一些who / tdr推薦認為很有希望的疫苗候選分子，如谷胱甘肽- s -轉移酶（ gst ） 、副肌球蛋白（ sm97 ） 、照射致弱疫苗抗原5 （ irv - 5 ） 、磷酸丙糖異構酶（ tpi ） 、曼氏血吸蟲膜內在蛋白（ sm23 ）和脂肪酸結合蛋白（ fabp ， sm14 ）等，但其對宿主的保護作用均不甚理想，未能穩定地達到40或以上的保護力水平，因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑，進一步提高其保護力。It is interesting that pma plus calcium ionophore a23187 can inhibit pma - induced pta1 expression, and this effect ca n ' t be reversed by calcmeurin inhibiter fk506. pta1 mabs can inhibit ctl activation and differentiation in mixed lymphocyte culture system when added at the beginning of the culture but can induce platelet activation and aggregation in the fc dependent manner. in 1997, pta1 cdna was cloned from cdna library of tpa activated jurkat cells, which belongs to immunoglobulin superfamily ( igsf ) with two v - like domains of extracelluar region of pta1
Il - 2 、 tnf - 、 pma可以使t細胞pta1表達上調， tgf -可以下調pta1的表達，而pma加上鈣離子載體a23187可以顯著抑制pma的上調作用，且這種抑制作用不被calcineurin抑制劑fk506所逆轉， 1997年burns教授從pma活化的jurkat細胞cdna文庫中克隆了pta1cdna全長，證實pta1是一個新分子，屬于免疫球蛋白超家族，胞膜外區有兩個v樣結構域。Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry. the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines, and compared with pgp inhibitor quinidine. the pgp expression of mcf - 7 adr was strongly positive, the positive rate was 15 % ; the pgp expression of mcf - 7 was negative, the positive rate was 1. 8 %. ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115. 2g ml and 207. 6g ml respectively. ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129. 8mol l 42. 1g ml and 94. 1mol l 30. 5g ml respectively. tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7, but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly. immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively. tea polyphenol is not only an anti - tumor agent, but also a multidrug resistant modulator similar as quinidine. tea polyphenol is advantageous for its little toxicity in tumor treatment
用免疫組化法和流式細胞儀對腫瘤細胞系mcf - 7和mcf - 7 adr的p -糖蛋白表達水平進行定性定量研究。用噻唑藍比色法mtt研究茶多酚的細胞毒性及其對耐藥性的逆轉作用，並與pgp抑制劑奎尼定進行了比較。免疫組化法檢測p -糖蛋白表達水平， mcf - 7 adr呈強陽性，而mcf - 7呈陰性流式細胞儀定量檢測結果mcf - 7 adr細胞系細胞陽性率為15 % ， mcf - 7細胞系細胞陽性率為1 . 8 % 。Aminated and hydroxylated polysulfone membranes were prepared by amination and hydroxylation reaction, respectively. then bovine albumin ( bsa ) - fixed membranes were obtained by crossed - linking albumin into porous membrane with 1, 1 ' - carbonyldiimidazole and bisoxirane reagents, respectively. a mathematical model for facilitated transport in asymmetric membranes with fixed site carriers was derived by assuming an instantaneous, microscopic concentraion fluctuation in the membrane
以氯甲基聚碸為基材，通過相轉化法制備出具有底部貫通孔的非對稱膜，通過胺化和羥基化反應，分別制得胺化聚碸膜和羥基化聚碸膜，再採用羰基二咪唑和雙環氧烷兩種活化試劑對其進行活化，將牛血清白蛋白固載在膜內，獲得固定白蛋白促進傳遞膜。The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition
本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因（ ssnhx1 ）的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那黴素的適宜濃度是50mg . l ~ （ - 1 ） 。The a - transglucosidase was selectively modified by pcmb, me, edc, clac, acetyl acetone and nbs, and changes in the activities of the enzyme have been detected. the reaction of a - transglucosidase with pcmb, me, edc and clac resulted in a strong inhibition of the enzyme activities which decreased with the increase of modifier concentration. the acetyl acetone and nbs were found without inhibition effect
酚丙酮、小涅代唬拍酷亞胺剛b引等化學修飾劑對a葡萄糖轉苦酶的幾種氨基酸殘基進行選擇修飾，並測定其酶活力變化，結果表明： pcmb 、 me 、 edc 、 ciac能顯著抑制酶的活性，活力的下降與修飾劑的濃度有關，乙酚丙酮、 nbs白巾飾對酶的奪製作用不明顯。The cowpea trypsin inhibitor ( cptt ) gene is testified as a broad spectrum insect - resistant gene at present and its application in insect - resistant botanic transgenic engineering only after s / gene. the cpti transgenic plant developed rapidly for it ' s broad spectrum insect - resistant character and the target insects are uneasy tolerance to it
豇豆胰蛋白酶抑制劑（ cpti ）基因是目前在植物抗蟲基因工程中應用僅次於bt基因的廣譜性抗蟲基因。鑒於它抗害蟲的廣譜性和靶標昆蟲不易對其產生耐受性的優點，轉cpti基因植物得到了迅速的發展。Levels of fasting blood glucose and 24h urinary microcontent of albumin 24 h malb were determined dynamically ; the serum glycosyl hemoglobin hba1c was determined after the last medication ; the ultrastructural changes of kidney were observed by transmission electron microscope ; the expressions of collagen, fibronctin, laminin ln, and the ecm metabolism influencing factors, including mmp2, tissue inhibitor of metalloproteinase timp2, transfer growth factor 1 tgf 1 in renal tissue were detected by immunohistological chemistry and image collecting analytical system
動態檢測各組大鼠空腹血糖fbg 24h尿微量白蛋白24h malb ，末次給藥后測定大鼠血漿糖化血紅蛋白hba1c透射電鏡觀察各組大鼠腎臟超微結構改變，應用免疫組化技術及圖像採集分析系統測定各組大鼠腎臟組織中型膠原c纖維連接蛋白fn層粘連蛋白ln的表達，測定影響ecm代謝的基質金屬蛋白酶2 mmp2基質金屬蛋白酶抑制劑2 timp2及轉化生長因子1 tgf 1的表達。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。Clinical trial of fritillaria thunbergii bulb powder for reversing multidrug resistance in the patients with acute leukemia
浙貝母散劑逆轉急性白血病多藥耐藥的臨床研究The results showed that in the beef cattle fed with chinese medicine additive, daily gain, number of lymphocyies and glucose and calcium concentrations in the blood were significantly increased, while npn content was decreased, some indexes, such as number of leucocyte, feed conversion rate and digestive rate of nutrients, had a tendency to rise, but there were no significant differences between treatment and control groups
結果表明，肉牛日糧中添加中藥飼料添加劑，可顯著提高日增重、血液中淋巴細胞數量、血糖和血鈣含量，非蛋白氮含量顯著下降；血液中白細胞、分葉細胞、採食量、飼料轉化率、各養分消化率均有上升趨勢，但組間無顯著差異。Tar content in the gas was influenced by the gasification conditions, such as the gasification temperature, residence time of feedstock in the gasifier and the type of feedstock, which can help us to investigate the mechanism of tar production in gasification process. catalytic cracking of tar was performed in a downstream secondary fixed - bed cracker with dolomite, limestone and alumina brick as catalysts. by comparison, thermal cracking of tar was also performed with silica carbide
在固定床二級催化裂化反應器上，實驗了白雲石、石灰石、高鋁磚等幾種催化劑作用下的焦油催化裂化過程以及炭化硅作用下的熱裂化過程，並對裂化溫度（ 650 950 ） 、氣相停留時間（ 0 . 5 1s ）和催化劑類型等過程參數對焦油轉化效果和熱解煤氣的影響進行了分析，對各種催化劑材料的性能進行了比較，力爭開發出可適用於工業化生物質氣化系統的焦油催化裂化技術。Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed
構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。Mitogen - activated protein ( map ) kinase signal transduction cascades are routes through which eukaryotic cells deliver extracellular messages to the cytosol and nucleus, and the increasing evidences showed that mapks are involved in aba -, sa - or h2o2 - signaling respectively. in addition, plant guard cells have been a well - developed model system for understanding how components interact within a signaling network in a single cell
本實驗在表皮生物分析的基礎上，主要利用顯微注射技術、膜片鉗技術和激光共聚焦顯微技術，運用專一性蛋白激酶抑制劑處理，探索蛋白激酶對蠶豆（ viciafabal . ）氣孔保衛細胞中aba和sa誘導的h _ 2o _ 2產生及其信號轉導影響機理，結果如下： 1By means of plant genetic engineering, foreign insects resistance gene can be transferred into plant cell. we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method. and obtained the transgenic mustard plants. the main results are as follows : 1. isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves. the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers
本實驗是利用植物基因工程獲得抗蟲的轉基因芥菜植株，結果如下： 1豇豆胰蛋白酶抑制劑基因的分離分別提取豇豆種子、子葉及葉片的總rna ，逆轉錄成cdna 。以豇豆胰蛋白酶抑制劑基因兩端的序列為引物，用rt - pcr的熱啟動方法從上述cdna中擴增出目的基因片斷。Study on angiotensin converting enzyme inhibitory peptides from digestion of soy protein in vitro
大豆蛋白體外酶解物中血管緊張素轉化酶抑制劑活性肽研究Gfp gene was also fused behind the signal peptide sequence to construct plasmid p3301ubisiggfp and transformed to tobacco. the results of fluorescent detection showed gfp localization in the apoplast
Elisa結果顯示，馬鈴薯蛋白酶抑制劑基因的信號肽序列使crylac基因在轉基因煙草中的表達量顯著提高。2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively
3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞，進行瞬時表達， 48小時后，用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。分享友人