轉白試驗 的英文怎麼說
中文拼音 [zhuǎnbáishìyàn]
轉白試驗
英文
blanching reaction-
Transgenic plants were comfirmed by molecular determination including pcr, southern blot and rt - pcr. 4. functional analysis and iron content of transgenic plant crude proteins of transgenic plant were extracted from leaves, and then used to test its antibiotic activities
4 .轉基因植株抗菌能力加強，鐵含量得到提高提取轉基因植株葉片粗蛋白，以大腸桿菌dhsq和酵母菌ahiog為試驗靶標，側定了轉基因植株粗蛋白的抑菌活性。The experimental results indicated that geotrichum candidum was an ideal microbe species which could produce 18. 6 % protein content after fermentation
試驗結果表明，白地霉對丟糟的轉化較為理想，發酵后可使蛋白質含量高達18 . 6 % 。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。705bp dna fragment of mxnrampl gene and full cdna of mxlrtl gene which were related to resist iron stress were cloned by using malus xiaojinensis cheng et jiang - the first iron - efficient genotype in the genus malus in the world as material. ( 1 ) using fragment of nramp gene from wheat and fe ( ll ) - transporter gene fragment of maize ( zmlrt ~ ) as probes, we analysed these genes by blotting hybridization technique in malus xiaojinensis cheng et jiang
本實驗以中國農業大學園藝植物研究所篩選到的一個蘋果鐵高效基因型? ?小金海棠（ malusxiaojinensischengetjiang ）為試材，分別克隆了小金海棠的抗缺鐵相關基因mxnramp1基因的752bp基因組dna片段和fe （ ） -轉運蛋白基因（ mxirt1 ）的cdna全長，為深入探討小金海棠抗缺鐵的分子機理奠定了基礎。The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus
結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene
因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。The experimental results showed that the various indexes such as biomass, enzyme activity, true protein content and so on through the rotating drum reactor were all superior to those through the rotating shaft reactor under the same experimental conditions
試驗結果表明在相同的試驗條件下，採用轉鼓式反應器進行發酵，產物中生物量、酶活、真蛋白質含量等各項指標均優于轉軸式反應器。The study was undertaken to isolate fe ( ii ) - transporter cdna and related binding protein cdna under fe - deficiency stress from a fe - deficiency root cdna expression library of malus xiaojinensis by screening library using maize fe ( ii ) - transporter cdna and wheat tamre - bp cdna as a probe. the main results as follows : 1 out of approximately 120000 plaques, four positive cdna clones encoding fe ( ii ) - transporter proteins, designated pftl -
本試驗以蘋果屬小金海棠（ malusxiaojinensischengetjiang ）為試材，利用玉米fe （ ）轉運蛋白基因片段以及小麥金屬反應元件結合蛋白tamre - bpcdna為探針，篩選小金海棠缺鐵根cdna表達文庫，目的在於克隆蘋果鐵高效基因型小金海棠的fe （ ）轉運蛋白基因以及與缺鐵脅迫相關的結合蛋白基因。There is no characteristic in the amino acid sequence 63 - 152 and it is the piece that we want to delete to identify the function of the segment. ie180 gene mutants deleted the 64 - 151 amino acid was amplified by muti - pcr and were cloned by pmdist - vector. the clone plasmids were named pjmp1p3p2. the segment corresponding to the sequence of 1 - 1079 amino acid of the genbank sequence amplified by pcr, its clone plasmids was named pjmp1p2. the segment corresponding to the sequence of 454 - 1079 of genbank sequence amplified by pcr and its clone plasmids is named pjmp6p2 - three clone plasmids and pcdna3 were digested by restriction enzyme bamhi and hind, the gene segment of p1p2, p1p3p2, p6p2 were recycled
本試驗應用dnamis及prosis軟體分別對genbank中登記號為no352564的ie180序列進行了蛋白質序列分析，結果表明其1 - 34段氨基酸序列具有典型helix - turn - helix特徵序列，並且富含酸性氨基酸d及e ，是典型的dna識別序列；富含絲氨酸序列的152 - 409氨基酸序列是一個與激活有關的、潛在的磷酸化位點； 454 - 696氨基酸區域是dna結合域； 64 - 151氨基酸片段沒有明顯的序列特徵；從中可看出ie180蛋白的1 - 1080氨基酸段具有典型的轉錄激活因子結構特徵。At the same time, it detailed demonstrate and carry out the way, which apply housing electric spindles at generation state to carry out housing toque loading in the joint testing and indirectly estimation housing torque loading by the electromagnetism torque of inner tube electric spindles. thus, the technical problem that the high speed shafting torque loading device and torque sender are difficult to ins tall is solved. and it saves a set of expensive high speed shafting torque loading and measuring device
同時，詳細論證並實施了採用外套電主軸工作在發電狀態實現接合試驗的外套扭矩加載和通過內套電主軸的電磁轉矩來間接估算外套加載扭矩的方法，從而解決了高速軸系扭矩加載裝置和扭矩傳感器不宜安裝的技術難題，並且節省了一套昂貴的高速軸系扭矩加載和檢測裝置，該成果填補了國內高速單向軸承試驗臺扭矩加載領域的空白，達到了國內領先水平。The immunomodulatory effects of crude product were detected by lymphocyte transformation test ( lit ) and plaque forming cell assay ( pfc )
Kp莢膜糖蛋白粗產品免疫功能檢測採用淋巴細胞轉化試驗（ mtt法）和溶血空斑試驗，分別測定細胞免疫和體液免疫功能。In this study, a series of plant expression vectors were constructed to comparing the expression level of ctb / cs3 in different organs, and transformed by co - cultivating leafdisc with agrobacterium strains lba4404 harboring target vector
為了優化表達系統，本試驗構建了分別將ctb cs3蛋白定位於細胞質、細胞間質和內質網的pbinctbatg cstaa 、 pbinctbsp cstaa 、 pbinctbsp cskdel植物表達載體及對照載體pbinctbatg cskdel ，分別通過農桿菌介導法轉化煙草。24, 48, 72 hours later after transfection, we testified the expression of pp38 with mab h19, and pp24 with the antiserurn of pp24 expressed in e. coli. the tests justified the expression of pp24 in prokaryotic and eukaryotic expressing systems. in order to study the correlation of pp38 and pp24, we cloned pp38 gene and pp24 gene into pbudce4
為研究pp38和pp24之間的關系，將型mdvmd11株的pp38基因和pp24基因的完整orf克隆到真核雙表達載體pbudce4 . 1中，轉染cef后，通過ifa檢測和用抗pp24多克隆血清進行western - blotting試驗檢測到了pp38和pp24磷蛋白的共表達。Introduced infected by the turnip mosaic virus. there are many useful objective genes can be used in vegetable genetic transformation, but the research work of chinese cabbage genetic transformation is little. the reaserch on transforming chinese cabbage using agrobacterium - med ated method has n ' t been report at home and abroad. ln the test, tumv - cp gene was transferred into chinese cabbage ( brassica campestris l. ssp. pekinensis ) via agrobacterium - mediated method. a high effective regeneration and genetic transformatin system has been established, the detection by the method of molecular biology, has proved that the regenerative plants are transgenic plants and the target genes have been expressed transgenic plants partly. meanwhile transgenic progenies were traced and investigated so that heredity, stability and expression of target gene were researched. the virus resistant, stable plants were expected to obt ain so that theoretical base can be established for chinese cabbage breeding by gene engineering
利用農桿菌介導法轉化大白菜抗病基因的研究工作在國內外未見報道。本課試驗採用農桿菌介導法將tumv - cp基因導入大白菜中，建立了高效的大白菜離體再生、遺傳轉化體系，並對轉基因植株進行分子生物學檢測，證實得到的再生植株為轉基因植株，目的基因已在部分植株上表達。同時，對轉基因植株的後代進行檢測，分析該基因所控制性狀的遺傳穩定性以及基因表達情況，為大白菜基因工程抗病育種提供理論依據。The trial was undertaken with the expectation that the higher hemoglobin target would be associated with better clinical outcomes ; however, the opposite effect was found
該試驗原本期望達到高血紅蛋白靶目標會伴隨著更好的臨床轉歸，結果卻截然相反。Based on the plant expression vector of pcambia1300 - hbmp - 3m and pcambial 301 - hbmp - 3 constructed, we set up the system of high frequency regeneration and transformation of tomato cotyledons. the hbmp - 3m gene and hbmp - 3 gene were respectively transformed into tomato and tobacco by transfection of agrobacterium tumefaciens. and the transgentic plants were detected. the main results are as follows : l. the plasmid pcambial300 - hbmp - 3m carried hbmp - 3m gene and pcambia - 1301 - hbmp - 3 carried hbmp - 3 gene were respectively transferred into agrobacterium tumefaciens by electroporation
本試驗是在已經構建好的植物表達載體pcambia1300 - hbmp - 3m和pcambia1301 - hbmp - 3載體的基礎上，建立番茄子葉的高頻率再生體系和番茄子葉的高頻遺傳轉化系統，利用根癌農桿菌介導的方法，將人骨形成蛋白- 3成熟肽（ hbmp - 3m ）基因和人骨形成蛋白- 3 （ hbmp - 3 ）全長基因分別導入番茄和煙草，並對轉基因植株進行了檢測。分享友人