通用連接組 的英文怎麼說

中文拼音 [tōngyòngliánjiēzǔ]
通用連接組 英文
typieal installation
  • 通 : 通量詞（用於動作）
  • 用 : Ⅰ動詞1 （使用） use; employ; apply 2 （多用於否定: 需要） need 3 （敬辭: 吃; 喝） eat; drink Ⅱ名...
  • 連 : Ⅰ動詞1 （連接） link; join; connect 2 （連累） involve （in trouble）; implicate 3 [方言] （縫） ...
  • 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 通用 : be in common use; be current; apply or be used universally
  • 連接 : connect; fit together; link; marry; mate; joint; association trail; linkage; concatenate; concate...
  1. The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation

    利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。
  2. 9 smith d, cypher a, spohrer j. kidsim : programming agents without a programming language. communications of the acm, july 1994, 37 : 54 - 67

    為支持組件之間的松耦合連接，該范型使用事件驅動中間件作為其底層的通信設施，並且採用基於事件驅動模型的construct定義組件之間交互。
  3. Total length flexes from 78cm to 130cm, the t shape pole is fixed with two extrusive slots respectively. the middle umbrella gear is connected with the pole. turn the mop pole to move the rail beside gear through the umbrella gear function

    ：總長度可由78公分伸縮拉長到130公分， 「 t 」形拖把桿上橫桿兩端分別有2個突出的卡槽中間一組傘形輪與拖把桿本體連接固定，轉動拖把桿通過傘形輪的作用「 t 」形拖把桿上輪兩端的橫桿即跟著傳動。
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  5. The sentry box of the activity fully use internal wall panel and one s own intensity of the roof boarding, through draw nail, bolt, connection nailed to make up finalize the design the prefab system. construction swift, being portable, especially suitable for urban municipal administration, entrance guard room, service department, etc

    活動崗亭是通過充分利用夾芯墻板及屋面板的自身強度，通過拉釘螺栓自攻釘將鋼結構底座墻面夾芯板和屋面板連接組成的房屋系統。
  6. The main research advances can be summarized as follows : ( 1 ) study the signal processing ' s performances and methods of homing torpedo system comprehensively, in order to setting up a corresponding mathematical models ; ( 2 ) analyze the ocean channel ' s effects on the work of homing system, then found some models such as target echo signal, noise ( including background noise, target radiating noise, etc ), ocean reverberation. according to them, simulate the array signal ; ( 3 ) the system structure, every function blocks composing are studied and founded thoroughly. then, discuss methods of signal processing in time domain and airspace domain ; ( 4 ) program the simulation software of torpedo ' s homing system according to the simulation models and flow charts, which connected with torpedo ' s control part

    本文所作的主要工作及研究成果主要有以下幾個方面： （ 1 ）對自導工作過程中的信號與信息處理的基本理論與方法進行了較為全面的研究，為建立一個較為完備的自導模擬系統提供了理論基礎； （ 2 ）討論了自導系統工作過程中海洋通道對目標回波信號與目標輻射噪聲信號等的影響，建立回波信號的數學模型、環境場中的噪聲信號模型（包括海洋環境噪聲、目標輻射噪聲與魚雷背景噪聲等）與海洋混響模型，模擬產生了聲自導系統基陣接收到的回波信號與噪聲信號； （ 3 ）深入研究並建立了自導模擬系統的總體框架，給出各個具體功能模塊組成，討論了聲自導系統對信號的時域與空域處理，並結合模擬程序中陣列信號處理模塊，給出固定多通道波束形成的模擬實現過程； （ 4 ）根據系統的模擬模型與已建立的模擬流程圖編制了通用魚雷模擬器自導系統模擬軟體，通過網路與控制系統相連，組成完整的魚雷模擬器。
  7. By transforming architecture elements ( component and connector ), we can get the process view of architecture. in the end we use the method to analyze the two cases in kwic system and compare the results of architecture performance analysis

    軟體體系結構由構件和連接件組成，本文分析了構成體系結構的六種組成元素，通過這些組成元素的轉化提取體系結構的進程視圖，然後應用排隊理論建立性能建模。
  8. Suitable for any type of conference. system includes operator system, vote system, vote host, vote machine, sign in card, control computer and so on. match the type of t connect mode, installed conveniently

    適用於任何大型會議場合，系統由操作軟體表決軟體表決主機表決器簽到卡控制電腦等組成，配以專用的「 t 」一線通連接方式，安裝方便。
  9. Because of the different function of roads and the difference of region, the form of intersections and connections between them is different with each other. at present, the types of nodes adopted in china and abroad include uncontrolled intersections, all - way stop - controlled intersections, two - way stop - controlled intersections, signalized intersections, roundabouts, grade - separated junctions and their combinations. each type of nodes has different characteristics and adapts to different function demands

    城市道路是以網路形態分佈於城市區域內的地面交通設施，由於道路的功用不同以及地域的差異，各道路間的交叉和連接方式各不相同，目前國內外所採用的節點形式有無控制平交節點、全停牌控制平交節點、二路停牌控制平交節點、信號燈控制平交節點、平面環行節點、立體交叉節點以及它們的組合形式，各種節點具有各自的特性，適合於不同的功能需求。
  10. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  11. Secondly, this paper made some theoretic researches on its engineering classfication and applicable scope for this technology ; then combining with project example, this paper carried out scheme design for this technology, and compared some different kinds of underpinning scheme and node and structure design, and put forward the method of " reinforcce steel bar through column " to build pile cap beam, " resistant bend and shear anchored reinforce steel bar " to strengthen column consolidation effect, and the method of " steel plate hoop " to build reinforcing bar connection of the foundation beam, etc. in the process of the consturction scheme research and implement, this paper synthetically elaborated the organizaton for project construction, put forward the construction technical measure of specific aim on the artificial pile, and pile cap beam, and underground adding layer and structure stabilization, and overall structure stabilization and so on. for this project, adopt reinforcing bar concrete to brace hole wall to ensure the safety of engineering in the artificial pile construction, use flexible connection catch to make the cage hoisting easier to simplify the construction process, use the method of earthwork statified symmetrical balance in the process of underground adding layer excavation

    本文首先對于基礎托換與結構加固技術的目的和意義、國內外發展狀況進行了綜合闡述；其次對該項技術工程分類及適應范圍進行了理論上的分析研究；然後結合工程實例對該項技術進行了方案設計，對比幾種不同的托換方案和節點及構造設計，提出了「通筋穿柱法」做承臺梁， 「抗彎抗剪錨筋法」強化柱加固效果， 「鋼板箍法」做地基梁鋼筋連接點等多項技術；在施工方案的研究和實施過程中，對于工程施工組織進行綜合闡述，並就人工挖孔樁、承臺梁、地下加層及結構加固、整體結構穩定等專項施工方案提出有針對性的施工技術措施，人工挖孔樁施工採用鋼筋砼護壁確保挖孔樁的安全成型，使用柔性連接鉤進行鋼筋籠吊裝簡化施工工序；承臺梁施工採用梁主筋橫穿柱身化學膠錨固等；地下加層綜合施工技術採用土方分層對稱平衡開挖，分段挖土做筏基結構自穩等；最後對于該工程實施后的效果分析，說明該項綜合施工技術的可行性。
  12. 2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates

    應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體，應用蛋白酶k法從病毒粒體中提取病毒rna基因組，根據已報道的tumv的cp基因序列，設計併合成了一對特異引物，利用rt - pcr法克隆了6個分離物的外殼蛋白基因，與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。
  13. The compartment modeling approach is likely more realistic for application, but further research is needed into the development of more appropriate dynamic and interactive connections, through which the multi - objects analysis can be solved based on a meaningful physical system

    組合模型更加容易應用，但是從長期的研究來看，需要一個適當的動態連接，通過內在的交互方法求解經濟和水文部分。
  14. Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues

    二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。
  15. It ' s well known that the project documentations " storage and management have an very important effect on the development of all kinds of project, and as the information age is coming, network technology has been applied to this procedure gradually. this article mainly introduces the study and realization of a internet - based project documentation storage and management system which application programming frame is built with delphi6. 0 and sql server2000. based on the tcp / ip protocol, the system try to establish a c / s model with connection - oriented, dependable stream sockets and multithread technology to complete some especial data communications by networks, such as the files of project, directory of user, all self - defined commands or messages and their parameters, and so on

    本文所研究的《基於internet的項目文檔存儲管理系統》通過delphi6 . 0和visualc + + 6 . 0相結合的開發平臺，結合sqlserver2000 ，將client server模式的數據庫技術和windowssocket網路通信技術進行集成，遵循tcp ip協議，同樣採用c s結構運行於internet ，通過服務器將處于各個不同物理位置的客戶機連接起來，形成一個交流平臺；其中，引入了目前國際上流行的先進的安全管理控制方法? ?角色控制理論對用戶進行權限分配，使擁有相應角色的人具有相應權限；然後系統自動根據組織部門或用戶的設立在服務器端生成相應的網路「虛擬文件櫃」 ，使擁有相應權限的人可以對相應文件櫃中的文檔進行整理；再通過多線程技術和windows套接字通信機制在tcp ip協議上實現了單個文件及目錄的傳輸。
  16. Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv

    方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。
  17. An often portable structure consisting of two long sides crossed by parallel rungs, used to climb up and down

    梯子一種通常是可移動的結構物，由長長的兩邊和兩邊平行的梯級連接組成，用於爬上爬下
  18. As such, dependencies are defined by wiring sca components together using references

    因此，通過使用引用連接sca組件，可以定義依賴項。
  19. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  20. Values that specifies a group of flags for controlling the advisory connection

    值，它指定用於控制通知連接的一組標志。
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