達瓜通 的英文怎麼說
中文拼音 [dáguātōng]
達瓜通
英文
takua thung-
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。While it is common for people from poorer countries to migrate to developed, wealthier nations, experts found there was also migration between developing countries - - for example, from guatemala to mexico
盡管人們從相對貧窮的國家移民至發達和更富有的國家是很普通的，專家發現發展中國家之間也存在移民現象，如從瓜地馬拉移往墨西哥。The fusing gene was transformed into leaf tissue of tobacco cultivar nc89. more than 100 plants were obtained. transgenes were confirmed to be inserted into tobacco genome of r0 generation by pcr and dot - blotting analysis
通過kpn位點與成熟番木瓜蛋白酶的cdna連成一個融合基因，並插入到pbi121的原gus位點，構建成nib -番木瓜蛋白酶融合基因的植物表達載體pnpa 。Plant expression vector lba4404 ( pbin + - 35s - cbf3 + pal4404 ) was used in the genetic transformation of cucumber and micro - tomato. establishment of high efficiency regeneration system and determination of parameters involved in agrobacterium - mediated transformation, screening and characterization of transformant were systemically researched
將植物表達載體pbin ~ + - 35s - cbf3對黃瓜和小番茄進行遺傳轉化，建立了黃瓜高頻再生和遺傳轉化體系，獲得了一株轉基因黃瓜植株，並通過了植物基因組pcr檢測；獲得了兩個具有kan抗性的小番茄再生綠芽。分享友人