達病毒 的英文怎麼說

中文拼音 [dábìngdú]
達病毒 英文
da virus
  • 達 : Ⅰ動詞1 （暢通） extend 2 （達到） reach; attain; amount to 3 （通曉; 明白） understand thoroughly...
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  1. Canine adenovirus type 2, cav

    表達盒克隆入犬2型腺病毒
  2. Expression and genetic immunization of hantaan virus g2 recombinant adenovirus

    2重組腺病毒的表達及其基因免疫的研究
  3. The hsp70 mrna level in the hepatopancreas of agonal shrimps infected with wssv by feeding was lower remarkably than that of normal shrimps, while no rule of hsp70 transcription was discovered in muscles of shrimps infected with vibrio anguillavium and wssv for different hours by injection

    但對經注射wssv病毒及焦傳珍編碼中國對蝦和凡納對蝦一種卜isp70的cona研究博士學位論文鰻弧菌后不同時間的對蝦肌肉組織進行hsl770mrna水平檢測，未發現兩種病原感染不同時間后hsl刃0表達的明顯規律。
  4. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  5. Overexpression of sweet potato feathery mottle virus coat protein in e. coli and preparation of its specific antiserum

    甘薯羽狀斑駁病毒外殼蛋白基因在大腸桿菌中的表達及特異抗血清的制備
  6. Viral infections are associated with up to 50% of asthmatic attacks in children.

    受氣喘病侵襲的兒童中，有高達50的人與病毒感染有關。
  7. The expression of viral antigens on the cell surface and disruption of the cytoskeleton can cause the cell - to - cell interactions and cellular appearance to change, making the cell a target for immune cytolysis

    病毒抗原在細胞表面表達，以及細胞骨架的破壞能引起細胞細胞相互作用，細胞的外形會改變，導致細胞成為免疫殺傷的靶位。
  8. Expression and purification of erns gene of classical swine fever virus in e. coli

    2基因在家蠶桿狀病毒表達系統中的融合表達
  9. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  10. The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks

    生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。
  11. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  12. The characteristics of tm - 22 expression presented in transgenic tobacco : 1 ). virus specificity in either homozygote or heterozygote ; 2 ) tm - 22 gene integrated in tobacco genomic dna with single copy and in inheritance and segregation to progenies on the mendel role ; 3 ). transgenic line with tm - 22 promoter ( ptm47 ) showed infected symptoms with cell death distinguished to one with 35s promoter ( ptm49 ) after inoculation with tomv - 2a

    其次，通過氨基酸序列和結構的比較，確定tm - 2 ~ 2基因的編碼蛋白與tomv病毒在抗病反應中相互識別的特異氨基酸及其功能；然後，應用重組dna技術，互換tm - 2 ~ 2基因和tm - 2基因的對應結構域，構建嵌合基因，獲得嵌合蛋白表達的轉化體，驗證tm - 2 ~ 2編碼蛋白中變異氨基酸的作用。
  13. Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified

    為建立對豬傳染性胃腸炎快速有效的診斷方法，並試圖在預防上提供有效的免疫制劑，本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化；並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。
  14. The virus may persist in the inoculation site for several days.

    這種病毒可留在接種部位達數天。
  15. Results of laboratory tests on blood samples from kampala and kamwenge performed by the centers for disease control and prevention ( cdc ), atlanta, usa have confirmed marburg virus infection in the putative index case, a mine worker, and in one of his close contacts during his illness

    美國亞特蘭大疾病控制與預防中心對烏干達坎帕拉居民與卡穆文奇居民的血樣進行了檢測，實驗檢測結果證實了所推斷的首個病例感染了馬爾堡病毒：一個礦工與他治療期接觸過的一個人。
  16. Construction of adenoviral vector for luciferase driven by htert core promoter modified with myc - responsive elements

    核心啟動子引導熒光素酶表達的腺病毒載體
  17. Construction and expression of rnase - resisting virus - like particles containing partial sequence of alpha - fetoprotein messenger rna

    部分序列的耐核糖核酸酶病毒樣顆粒的構建和表達
  18. The outbreak was traced to infected vervet monkeys cut up in the labs for polio research ; they came from close to the present outbreak in uganda

    其病毒的蔓延可追溯到在實驗室因脊髓灰質炎的研究而致死的受感染的黑長尾猴，這些猴子來自最近爆發疾病的烏干達。
  19. The purpose of this study was to clone the major structural protein vp3 gene of gpv hl isolate after pcr, and express by protokaryotic and eukaryotic expressing system, then develop molecuiar diagnostic reagent of goose piaque and construct recombillat fowlpox vina life vector vaccine

    利用pcr方法擴增和克隆gpvh1分離株主要免疫原性蛋白基因vp3 ，並對其進行原核和真核表達，是建立小鵝瘟分子診斷方法、構建vp3基因重組禽痘病毒活載體疫苗的基礎，具有極為重要理論和實踐意義。
  20. In order to breed maize dwarf mosaic disease ? esistance maize species, the pap gene was introduced into maize inbred strains and hybrid strains mediated by pollen and particle bombardment, 10 transgenic maize plants were achieved and the pap gene was indeed integrated into the maize genome by analysis of molecular biotechnological methods. the pap gene in one of the transgenic maize plants had been transcription and translated, the ability of the virus resistance is undergoing

    為了解決玉米矮花葉病害的防治問題，採用花粉介導法及基因槍法將pap基因分別導入玉米的雜交種及自交系，獲得了10株轉基因玉米， pcr及southern - blot檢測結果表明pap基因已經整合到了玉米基因組中，並且其中有一株pap基因已經得到了表達，病毒抗性鑒定表明轉基因玉米能明顯推遲玉米矮花葉病害癥狀的表現。
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