遺傳載體 的英文怎麼說

中文拼音 [yízhuànzǎitǐ]
遺傳載體 英文
genetic carrier
  • 遺 : 遺動詞[書面語] （贈與） offer as a gift; make a present of sth : 遺之千金 present sb with a gener...
  • 傳 : 傳名詞1 （解釋經文的著作） commentaries on classics 2 （傳記） biography 3 （敘述歷史故事的作品）...
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 遺傳 : [生物學] heredity; hereditary; inheritance; inherit
  • 載體 : [化學] carrier; supporter; isotopic carrier
  1. But more and more studies showed that iron - overload was involved in many diseases ( such as atherosclerosis, ischemia / reperfusion injure, hereditary hemochromatosis, and so on )

    然而近來的研究發現，鐵超載在機體一些疾病（如動脈粥樣硬化、心肌缺血復灌性損傷、遺傳性血色素病）的發展過程中起了重要的作用。
  2. Many studies show that leafy is high homolog even among distantly related plant species. exception of these, little studies on tissue culture and transformation of ginkgo have been done. this paper emphasizes on the isolation, cloning and analysing two ginkgo orthologs of leafy from the male tree

    為此，本實驗從銀杏leafy同源基因的克隆入手，分析其雌雄株lfy基因結構差異，構建lfy基因的植物正義反義表達載體，建立矮牽牛遺傳轉化體系，以研究銀杏lfy同源基因的功能，同時建立了銀杏組織培養體系，為銀杏的遺傳轉化和提早開花結果奠定基礎。
  3. Selection maskers are the important ingredient of the vector, and it is also a hot research spot of the genetics of lactic acid bacteria at present

    篩選標記是載體的重要組成部分，也是目前乳酸菌遺傳學研究領域的一個熱點。
  4. This paper describes a strategy that has developed to transfer the cdna of tobacco mnsod gene into the commercially important breeding line - baoding alfalfa via agrobacterium infection. transgenic alfalfa plants have been generated that overproduce a nicotiana plumbaginifolia l. manganese superoxide dismutase ( mnsod ). the results domenstrated that baoding alfalfa is an important breeding line which easily amenable to genetic transformation

    本研究採用我國農藝性狀優良的豐產苜蓿品種保定苜蓿，通過農桿菌介導的轉基因方法，使用特定的質粒載體pchlsod將煙草mnsod基因的cdna序列導入保定苜蓿中，說明保定苜蓿是一種易於遺傳轉化的優良苜蓿育種品系。
  5. As a popular vegetable, tomato is rich in vatamin a, b, p and other nutrients ; among them, lyxopene can prevent prostate gland tumor. so introducing stilbene synthase gene into tomato to get new health - protection tomato will have important social and economic effect. our research is made up of four parts ; the cloning and sequencing of stilbene synthase ; tomato genetic transforming mediated by agrobacterium - lba4404 ; quantification of resveratrol in transgenic tomato by hplc analysis

    本研究包括四個方面的內容：芪合酶基因（ stilbenesynthasegene ）的克隆與全序列分析；芪合酶基因（ stilbenesynthasegene ）植物表達載體的構建；農桿菌lba4404介導的番茄遺傳轉化： hplc技術分析轉基因植株中表達白藜蘆醇的含量。
  6. Leaves of tobacco ( nicotiana tabacum ) were wounded, infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring expression vector pbgsb and pbglsb and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 5mg / l 6 - ba + 0. 7 % agor ph 5. 7 )

    4以煙草（品種sr ）無菌苗葉片為外植體，採用農桿菌浸染的葉盤轉化法，用構建好的植物表達載體對煙草進行遺傳轉化。外植體置於無抗生素的誘芽培養基（ ms 0
  7. Construction and transformation of inverted repeat of turnip mosaic virus coat protein gene

    基因反向重復植物表達載體的構建及遺傳轉化
  8. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  9. Armed with modified antibacterial peptide ( spcema and mcema ) and plant defensin afp, transgenic plants with broad - spectrum and strong disease resistance may be obtained

    以上三個單價和一個雙價抗菌肽表達載體在植物中的遺傳轉化，有望獲得對病源真菌有廣譜抗性的轉基因植株。
  10. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。
  11. Construction of plant expression vector with human endostatin gene and transformation of tobacco

    利用人內皮抑素基因構建植物表達載體及對煙草的遺傳轉化
  12. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  13. Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )

    將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中，導入根瘤農桿菌gv3101后，由花浸泡法進行擬南芥遺傳轉化，轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素（ 25mg ）的ms培養基上篩選，獲得t ；代轉基因植株。
  14. Of the species, . characters of ecology, reproductive biology, genetic diversity, population genetic structure, endangering mechanisms, and sampling strategy have been studied in view of conservation biology. the results may be summarized as follows : by field investigation, we found that the localities recorded for four specimens of d. versipellis and d. pleiantha had disappeared, and most of the present populations are located in protected subforests. the distributional range of d. versipellis has also been reduced greatly, evidently, the number of present populations has decreased, the resources are reduced sharply, and the species are clearly endangered

    從保育生物學的角度，對八角蓮種群生態學和生物學特性、遺傳多樣性水平和遺傳結構、瀕危機制及保育取樣策略進行了研究，結果如下：通過野外調查發現，原標本記載的一些八角蓮和六角蓮種群已難以找到，該類群目前多分佈於自然保護區外圍次生林地帶，分佈范圍明顯縮小，現存群體的規模也明顯減少，資源量貧乏，物種處于瀕危狀況。
  15. Site experiment were carried out on the testing vehicle for ahs and the magnetic markers road in national intelligent transport system center. also, study on multi - sensor information fusion and control model by using neural net system, fuzzy logic and genetic algorithm were carried out. the work completed are as follows mainly : ( 1 ) hardware system for lane keeping system including magnetic marker, magnetic sensor, control system and executor machine were designed

    本文以國家智能運輸系統研究中心的自動公路系統試驗車和專用的磁軌釘道路為工作平臺，在大量的現場實驗基礎上，針對基於磁軌釘導航的車輛車道保持技術中的若干問題展開研究，運用神經網路、模糊邏輯、遺傳演算法等方法在多傳感器信息融合、控制模型等方面進行了系統的研究，主要完成了以下幾方面的工作： （ 1 ）對車道保持系統硬體平臺進行了設計，論述了導航設施? ?磁軌釘的設計過程和車載工控機、步進電機等的選擇。
  16. The chromosome is the carrier of genetic material. there are genes on it. it decides human being ’ s configuration and physiological function

    染色體是遺傳物質的載體，它上面帶有遺傳因子，決定人體的形態特徵和生理機能。
  17. Two strains of prrsv were isolated from the swine infected with prrsv in shangdong province and daqing area, in order to clarify the source and genetic background of porcine reproductive and respiratory syndrome virus ( prrsv ) from different parts of china, thus providing theoretic basis for the study of vaccine against it. the prrsv was cultured on mark - 145 cells for 5 ~ ~ 6 passages. when the cpe was obvious, the virus was harvested and purified

    為了弄清我國不同地區prrsv的來源以及其遺傳學背景，為疫苗學研究提供理論根據，本研究在ch - 1a株完整的基因組獲得以後，從流行於我國山東（ sd ）和黑龍江大慶（ dq ）地區疑似prrs的豬體內分離到prrsv ，在mark - 145細胞上盲傳5 6代，細胞出現明顯病變以後，收獲病毒液，然後提純，提取全病毒rna ，經過反轉錄、 pcr擴增獲得結構基因orf2 7的目的基因片斷，然後與pmd - t載體連接，轉化，得到陽性質粒后進行測序，並將其與ch - 1a株進行了比較分析，同時對這兩個毒株的結構基因組的理化性質進行分析。
  18. The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin

    本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。
  19. Such information is first placed into the ascension grid work, which then is downloaded to the genetic grid work by the angels and divas involved with the weaving of form

    這類信息首先被放置到提升晶格層中，然後由與身體編織有關的天使和提婆們下載到遺傳晶格層中。
  20. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
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