酰化酶 的英文怎麼說

中文拼音 [huà]
酰化酶 英文
acylase
  • 酰 : 名詞[化學] （酰基） acyl
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  1. E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd

    以構建的噬菌粒psurfpga轉化具有琥珀突變的大腸桿菌xl1 - blue ，以輔助噬菌體m13k07超感染，進行青霉素g酰化酶基因的表達和在噬菌體表面的展示。
  2. In these chloroplasts carbon dioxide combines with phosphoenolpyruvate to form oxaloacetic acid, which is transported to the bundle sheath cells, where the carbon dioxide is released, then fixed by the enzyme ribulose bisphosphate carboxylase to form glycerate 3 - phosphate, the first step in the calvin cycle

    在葉肉細胞的葉綠體中二氧化碳與磷酸烯醇丙酮酸結合形成草酰乙酸，后被運到鄰近的維管束鞘細胞，在那裡二氧化碳被釋放，后被核酮糖二磷酸羧化酶固定形成3磷酸甘油酸，這是卡爾文的循環第一步。
  3. Studies of aminoacylase immobilized on macroporous resin

    固定化氨基酰化酶的研究
  4. Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker

    本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。
  5. Localizaton of activity of immobilized penicillin gacylase by x - ray microanalysis

    酰化酶活性的定位
  6. A new carrier for immobilization of penicillin acylase

    固定化青霉素酰化酶的新載體
  7. Progress in support material for the immobilization of penicillin acylase

    青霉素酰化酶固定化載體材料研究進展
  8. Studies on immobilization of penicillin acylase to chitosan as larrier

    以殼聚糖為載體固定化青霉素酰化酶的研究
  9. The purified enzyme had a specific activity of 68. 6 u / mg protein. overproduction of pga was often limited by translocation and / or periplasmic processing steps, subsequently resulted in intracellular accumulation of various types of pga precursors and then formed inclusion bodies in the cytoplasm and / or periplasm

    經deae - sepharosecl6b離子交換層析和butyl - sepharosecl4b疏水層析，即可得純度提高20倍、比活為68 . 6u mg的青霉素g酰化酶，兩步純化的總得率達91 。
  10. Immobilization of penicillin acylase by intensive adsorption of chitosan on celite

    硅藻土強化吸附殼聚糖固定化青霉素酰化酶
  11. Immobilization of penicillin g amidase on beaded glycidyl methacrylate copolymer support

    青霉素酰化酶在甲基丙烯酸縮水甘油酯共聚物上的固定化
  12. The supernatant fraction and the precipitation fractions were analyzed by western blotfor strain dh5 a / pkkfpga, 5 - 10 % pga precursors formed as inclusion bodies in the cytoplasm while no inclusion bodies formed in the periplasm, this suggested most pga precursors were transported to the periplasm and matured to active pga and indicated that the maturation of pga in strain dh5 / pkkfpga was limited by the translocation step

    Western印跡分析表明對于菌株dh5 pkkfpga ， 5 - 10的原前體青黴g素酰化酶在胞內形成了包涵體，說明其成熟的限速步驟在胞內的運輸階段，而菌株dh5 psmlfpga則無明顯包涵體形成，說明菌株dh5 psmlfpga改善了青霉素g酰化酶的合成流，因而其表達能力高於菌株dh5 pkkfpga 。
  13. Studies of a polyacrylic acid as a carrier for the immobilizat ion of penicillin acylase

    聚丙烯酸載體用於青霉素酰化酶的固定
  14. Studies on immobilization of penicillin acylase on novel complex carrier pei silica gel

    青霉素酰化酶在新型復合載體上的固定化研究
  15. Emmobilzation studies of penicillin acylase on the porous bead with oxirane groups

    以環氧乙烷為活性基的多孔顆粒狀固定化青霉素酰化酶的制備
  16. Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g

    為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株，在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術，通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ，將其克隆到puc19載體中，經酶切鑒定、 pcr鑒定篩選出重組陽性質粒，並測序鑒定，通過美國blast程序進行了基因數據庫相似性比較分析。
  17. A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein. the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis. it was demonstrated that constructed phagemid can still express penicillin g acylase

    將包含信號肽和琥珀終止密碼子uag （ amber ）的完整巨大芽孢桿菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ，通過引入的11肽連接青霉素g酰化酶的c末端與噬菌體外殼蛋白gp3的n末端。
  18. 1, the histone deacetylase inhibitors were used to feed the larve of the fly, and then the polytene chromosomes were observed under the microscope. the results indicated that the histone deacetylase inhibitors had effects on the morphology of the polytene chromosomes. it is suggested that the acetytion has effects not only on the molecular interaction but also on the structure of the chromosomes

    得到的主要結果和結論如下： 1 ，通過用去乙酰化酶抑制劑處理果蠅幼蟲，觀察果蠅多線染色體在熱休克基因處的形態變化，發現去乙酰化酶抑制劑介導的乙酰化水平的升高可引起染色體結構的顯著變化，推測乙酰化修飾不僅影響分子間的相互作用而且還可以影響染色體的高級結構。
  19. 2, the hsp gene transcription was quantitatively determined by rt - pcr. based on this result, it is concluded that the change of acetylation level at the loci of hsp, mediated by histone deacetylase inhibitors, exerts important functions in hsp gene transcription. 3, after immunolabeling with anti acelated - lysine monoclonal antibody on the polytene chromosomes of heat shocked flies, fluorescence signals were detected at the hsp loci

    2 ，利用rt - pcr技術，對去乙酰化酶抑制劑處理后的果蠅的熱休克基因的表達水平進行檢測，結果表明經組蛋白去乙酰化酶抑制劑處理后的果蠅幼蟲，其熱休克基因的表達高於基礎水平，也就是說去乙酰化酶抑制劑誘導了熱休克基因的表達。
  20. The display of penicillin g acylase from bacillus megaterium not only offers the possibility of applying this technology for the selection of penicillin acylases with new side - chain specificities, but also facilitates our screen of mutant library constructed by using dna shuffling technique

    這是首次在噬菌體表面展示出有活力的巨大芽孢桿菌青霉素g酰化酶，為利用噬菌體展示技術進行青霉素g酰化酶突變庫的篩選奠定了基礎。
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