酵素母質 的英文怎麼說
中文拼音 [jiàosùmǔzhí]
酵素母質
英文
zymogen-
Fish meal, corn gluten, wheat meal, krill meal, yeast, wheat germ ， fish oil, spirulina powder, vimamin ? and mineral permix, glucan
魚粉、小麥粉、磷蝦粉、酵母、麥胚芽、魚油、螺旋藻粉、復合維生素、礦物質、卵磷脂、葡聚糖等。Fish meal, corn gluten, wheat meal, krill meal, yeast, wheat germ ， fish oil, spirulina powder, vimamin and mineral permix, glucan
魚粉、小麥粉、磷蝦粉、酵母、麥胚芽、魚油、螺旋藻粉、復合維生素、礦物質、卵磷脂、葡聚糖等。Krill meal, fish meal, squid liver powder, mussel meal, liver meal, brewer yeast, spirulina, wheat flour, seaweed powder, pearl powder, astaxanthin, - glucan, vitamin and trace minerals
磷蝦肉，魚肉，烏賊肝末粉，牡蠣肉，肝臟，啤酒酵母，藍綠藻，麵筋粉，海藻粉，珍珠粉，蝦紅素， -多醣體，維他命及微量礦物質。Nutripet is used for many types of disorders, such as rickets, osteomalacia, retarded growth, digestive disorder, hepatic insufficiency, dermatitis, lack of appetite etc. that derive from deficiencies of calcium, phosphorous, trace minerals and vitamins, reinforces the vitality, healthiness and improves coat condition
育犬精- f是一個給貓和狗最好的多功能礦物質輔助品，它富含多種維他命肝精粉微量礦物質甲殼素蝦紅素- 1 ， 3 -多醣體，酵母粉及高含量的鈣與磷The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。For dogs, simply replace the vegecat mentioned below with vegedog. the vegecat vegekit, vegeyeast and digestive enzymes are necessary because, like meat, vegecat and vegekit contain taurine, which cats and kittens need to avoid blindness and heart failure. the vegeyeast supplies protein, b vitamins, flavor and a high acid content to prevent urinary failure, and the enzymes are living digestive enzymes that cats need for digestion
食用成幼貓用素食vegecat vegekit素酵母vegeyeast和素消化道酵素digestive enzymes對貓狗很必要，因為成貓用素食vegecat和幼貓用素食vegekit含有肉類中含有的牛黃酸，可防止成貓和幼貓患眼盲及心臟病素酵母vegeyeast含有蛋白質維他命群調味料和高酸性成分，可避免泌尿器官的病變而素消化道酵素digestive enzymes是貓消化時所需要的活性消化酵素。Quality beef ， chicken, egg ， ocean fish, meat meals, fish meals, white fish meals, taurine, soy beans, yeast, rice, whole corn, wheat flour, animal fat ， plant oil, vegetables, fibers ， vitamins & minerals ， antioxidants
精選牛肉、雞肉、雞蛋、海洋魚、肉粉、魚粉、白魚粉、牛磺酸、大豆、酵母、大米、玉米、小麥粉、植物油、動物脂肪、鮮蔬菜汁、膳食纖維、復合維生素和礦物質、抗氧化劑。Quality beef ， chicken, egg ， ocean fish, meat meals, fish meals, white fish meals, taurine, soy beans, yeast, rice, whole corn, wheat flour, animal fat ， plant oil, vegetables, fibers ， vitamins & amp; minerals ， antioxidants
精選牛肉、雞肉、雞蛋、海洋魚、肉粉、魚粉、白魚粉、牛磺酸、大豆、酵母、大米、玉米、小麥粉、植物油、動物脂肪、鮮蔬菜汁、膳食纖維、復合維生素和礦物質、抗氧化劑。Gap is a homogenic microencapsule consisting of a consolidated nutrient and is not a mixture. its ingredient include ; whole egg, shrimp head extract, casein, yeast extract, cholesterol, lecithin, enzyme, polyunstaurated fatty acid, trace minerals, vitamins
酪蛋白鮮蛋液魚肉蛋白分解物酵母抽取物幾丁質膽固醇磷酯脂酵素高級不飽和脂肪酸epa微量礦物質綜合維他命及其他未知生長因素u . g . f 。Brain fuel contains no : yeast, salt, sugar, gluten, dairy, starch, soy, animal products, artificial preservatives or coloring
腦活素不含：酵母、食鹽、糖、麩質、奶油、澱粉、豆類、動物產品、人工防腐劑、色素。Although yeast cannot digest cellulose or lignin, the molecules that form a plant ' s skeleton, some bacteria and other species of fungi are able to do the job
雖然那些組成植物莖干纖維素和木質素的分子不能為酵母所消化，但是某些細菌和其他種類的真菌可以完成這項任務。Protein and nucleic acid in yeast cells were degraded, then the product was refined into nutritional seasoning, which was in step with current tide of nutrition and nature
摘要酵母抽提物亦稱酵母味素，是將酵母細胞內蛋白質、核酸等進行生物降解，經加工而成的營養型天然調味料，符合目前「天然、營養、回歸自然」之潮流。A study of yeasts during the delignification and fungal transformation of wood into cattle feed in chilean rain forest
中譯：酵母在智利原生平原雨林牛隻飼料中進行木質的去木質素作用及真菌類轉換等的相關研究。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。Then, tlc was used to purify them, and antibiotic experiments were made to define which was the main antibiotic substance. results showed, orange pigment was the major antibiotic substance, and it could inhibit bacteria, but had no effect on yeasts and algae. the diameter of the inhibition zone was directly proportional to the value of absorption of orange pigment
（ 2 ）利用吸附柱分離三種色素，並用tlc法純化，刮取相應的色素點， 70乙醇溶解，濃縮，進行抑菌實驗，證實橙色素是主要的抑菌物質，對細菌具有較強的抑制效果，其抑菌性與其吸光度呈正比變化，橙色素對酵母菌和黴菌無抑制效果。分享友人