酶原粒 的英文怎麼說

中文拼音 [yuánlì]
酶原粒 英文
zymogen granules
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 原 : Ⅰ形容詞1 （最初的; 原來的） primary; original; former 2 （沒有加工的） unprocessed; raw Ⅱ動詞（原...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  1. The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame

    重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。
  2. The capybara, nearly the size of a grown human, was not expressing telomerase, suggesting evolution was willing to forgo the benefits in order to reign in cancer

    幾乎和成人體重相當的水豚並不表達端粒酶，說明進化還是更樂于放棄復原的益處而到達對癌癥的控制。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. On the other hand, - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established. methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation. this vector is used to pronucleus microinjection

    本實驗以pegfp - n1質粒為骨架載體，用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體，雙切線性化后回收，使用回收的表達載體經原核顯微注射生產轉基因小鼠。
  5. In this paper, the relationship between cyanide - resistant respiration and expression of alternative oxidase ( aox ) using polyantibodies prepared by synthetic polypeptide in mung bean seedling, as well as the effects of vernalization on cyanide - resistant respiration and expression of aox in winter wheat were studied respectively. in the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic s helix of aox. were synthesized by solid - phase method

    第一部分，我們按照交替氧化酶（ alternativeoxidase ， aox ）位於線粒體內膜外側親水區s螺旋的氨基酸序列，用固相法合成由12個氨基酸殘基組成的多肽，將此多肽與-牛胰凝乳蛋白酶原a相連，用作半抗原，免疫家兔制備人工合成12肽的抗體。
  6. There are very developed rough surfaced endoplasmic reticulums ( rer ) which was lamellar arrange in cytoplasm of acinar cells and there are much zymogen granules in cytoplasm where approach the lumen of acinus under the electron microscope

    電鏡下，胰腺泡細胞內有非常發達的粗面內質網，排列呈板層狀，靠近腺泡腔的胞質中有許多大而圓的酶原顆粒。
  7. Renin, a proteolytic enzyme formed in the granules of the juxtaglomerular apparatus cells, catalyzes conversion of the protein angiotensinogen to angiotensin i, a decapeptide

    腎素是腎小球旁體細胞顆粒內形成的一種蛋白水解酶，催化蛋白血管緊張素原轉換為血管緊張素，即十肽。
  8. When sparsely - planted rice overgrew its earing period, light intensity in the mid - lower part increased, photosynthesis prolonged, activity of nitrate and peroxide decreased, protein content increased by more than 50 %, velocity of leaf ageing slowed, but accumulation of dry matter of grain accelerated and 1000 - grain weight increased too

    摘要稀植栽培的水稻，抽穗后田間表現為中下部光強明顯增加，光合作用時間延長，硝酸還原酶和過氧化物酶活性降低，蛋白質含量增加50 %以上，葉片衰老延緩，籽粒干物質積累加速，千粒重提高。
  9. Nucleic acid sequences of azoreduclase were searched and blasted in genbank. a pair of primers based on the conserved regions were designed. a specific fragment was amplified by pcr from the plasmid of rhodopsedomonas palustris and sequenced. the sequence contained a complete 471bp orf ( open reading frame )

    脫色實驗證明沼澤紅假單胞菌（ rhodopsedomonaspalustris ）對偶氮染料有較強的降解能力，我們通過genbank搜索，對所獲得的所有偶氮還原酶基因在ncbi進行比對並設計引物，從沼澤紅假單胞菌質粒中擴增獲得了一條含471bp完整開放閱讀框架的序列。
  10. Methods : thirty - two patient samples of primary head and neck squamous cell carcinoma and 15 adjacent tissues were assayed using trap - silver staining for detection of telomerase activity

    方法：採用trap銀染法，對32例原發頭頸部鱗癌及15例癌旁組織進行端粒酶活性檢測。
  11. As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )

    為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。
  12. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  13. When inadequate n was added, the chloroplast structure in mesophyll cell was damaged in ear leaf, the amount of carbohydrate decreased in mestome sheath, and excessive n - redistribution and n - transportation to grain from vegetative mass appeared, which resulted in earlier leaf senescence. excessive n application led to too high activity of nitrate reductase, excessive vigorous nitrogen metabolism and too much exhaustion of carbohydrate in ear leaf, which resulted in the lack of enough carbohydrate in the lower leaf, meanwhile, the expansive chloroplast grana lamellae in leaf mesophyll cell and starch grain without nuclear in mestome sheath cell was observed, which led to the decrease of chlorophyll content and photosythetic capacity in maize leaf, then the early senescence occured

    氮肥用量不足導致穗葉葉肉細胞葉綠體結構性差，維管束鞘細胞碳水化合物累積減少，營養體氮素再分配率大而引起葉片早衰；而過量供氮則導致生長後期硝酸還原酶活性過高，氮素代謝過旺，消耗了大量碳水化合物，以致下位葉不能得到充足的碳水化合物供應而提早脫落，同時葉肉細胞葉綠體片層結構膨脹，呈「肉汁化」特徵，維管束鞘細胞澱粉粒大量消耗，無核澱粉粒出現，從而葉片葉綠素含量下降，光合能力降低而出現早衰。
  14. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  15. Acid protease gave play to synergetic action in liquor fermentation manifested as dissolving the granules of fermentation materials, advancing microbial propagation, decomposing protein to produce flavoring materials, and degrading yeast tropina

    摘要酒用酸性蛋白酶在白酒釀造的發酵過程中起協同作用，具有溶解發酵原料的顆粒、促進微生物繁殖、分解蛋白質生成香味物質、降解酵母菌體蛋白等多種功能。
  16. To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully

    本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。
  17. Selected one of the 14 strains - s93, s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18, then transformed into dh5 a. filtering the clone with hybridization in situ, a 1 kb frament clone has been cloned

    使用sau3ai對基因組dna進行不完全酶切，回收2 3kb片段，與puc18質粒連接轉化大腸桿菌，利用地高辛標記探針，使用菌落原位雜交篩選轉化子；篩選到包含有約1kb外源片段的轉化子。
  18. Objective, clone tissue - type plasminogen activator ( t - pa ) gene and construct a new kind of recombinant vector containing human tissue - type plasminogon activator ( t - pa ) cnda neither cytotoxiaty nor actovating prot - oncogenes

    目的：克隆組織纖溶酶原激活物（ t - pa ）基因並構建一種無細胞毒性、不激活原癌基因的真核表達的pcdna3 . 1 （ + ） / t - pa質粒載體。
  19. A 1. 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites. the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge. strain dh5a of e. coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so

    以質粒ppgedna為模板， pcr擴增出1 . 7kb的ge基因完整片段，將擴增產物以ecori和bamhi雙酶切后，插入原核表達載體pbv220的p _ rp _ l啟動子下游的ecori和bamhi位點間，得到重組表達質粒pbvge ，轉化了pbvge的大腸桿菌dh5a經溫敏誘導表達后，用sds - page和western - blot ，以及瓊脂雙擴散來檢測，結果表明prvfa株ge基因在原核載體上得到高效表達，表達產物約占總蛋白的17 。
  20. Effect of adjusted zuojin pills on proliferating cell nuclear antigen and telomerase activity in rats with gastric precancerous lesion

    加味左金丸對大鼠胃癌前病變增殖細胞核抗原和端粒酶活性的影響
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