酶復合體制備 的英文怎麼說

中文拼音 [fùgětǐzhìbèi]
酶復合體制備 英文
enzyme complex preparation
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 復 : Ⅰ形容詞1 （重復） repeated; double; duplicate 2 （繁復） complex; compound Ⅱ動詞1 （轉過去或轉過...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 體 : 體構詞成分。
  • 制 : Ⅰ動詞1 （製造） make; manufacture 2 （擬訂; 規定） draw up; establish 3 （用強力約束; 限定; 管束...
  • 備 : Ⅰ動詞1 （具備; 具有） have; be equipped with 2 （準備） prepare; provide with; get ready 3 （防備...
  1. The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )

    以抗h3n2流感病毒的多克隆抗體（ 100ng l ）包被酶標板，加入制備好的肽庫，用tbst洗去非特異結合的噬菌體，加0 . 2mol l甘氨酸-鹽酸（ ph2 . 2 ） ，室溫放置10min以洗脫特異結合的噬菌體， 2mol ltris - hcl （ ph9 . 1 ）中和后，取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定，其餘噬菌體擴增後用于下一輪篩選，共重復3輪淘洗。
  2. Finally enzyme is covalently immobilized. the reclaimed efficiency of immobilized enzyme that is prepared by this vector is over 76 %, but others are between 45 % and 65 %. it shows that activated magnetic polymer composite microspheres is an efficient vector that can be applied immobilization field

    通過這類載體制備的固定化酶的酶活回收率在76以上，而由一般載體制備的固定化酶的酶活回收率在45 - 65之間，這表明活化后的磁性高分子復合微球是一類可以應用於酶固定化領域的高效載體。
  3. In vitro injury models of brain slice ( ogd and nmda insult ) and primary neuronal cultures ( nmda insult ) oxygen / glucose deprivation ( ogd ) - induced injury of rat hippocampal slice in vitro the rat hippocampal slices prepared were allowed to recover in the normal artificial cerebrospinal fluid ( acsf ) bubbled with gas mixture of 95 % o2 + 5 % co2 for 1 h, then they were thansfered to glucose - free nacsf which was bubbled with gas mixture of 95 % n2 + 5 % co2. after treatment with ogd, the slices were placed into 2 % ttc solution in dark and incubated at 37 * cfor 1h. the slices were weighted and a 50 : 50 mixture of ethanol / dimethyl sulfoxide was then added to extract the formazan in dark for 24 h

    離體腦片損傷模型（ ogd和nmda ）及原代培養神經元nmda損傷大鼠離體海馬腦片缺氧缺糖（ ogd ）損傷大鼠離體海馬腦片制備后，在通氧混合氣的正常腦脊液（ nacsf ）中恢復60min ，然後移入通氮混合氣的無糖腦脊液（吵化sf ）中缺氧缺糖，取出腦片與2 ttc避光37 』 c溫浴60dll ，染色后根據每克濕重加入20ml抽提液（乙醇：二甲亞礬一50 ： 50 ） ，在密閉容器內避光置24h ，測量前搖勻后取200pi至96孔板，在490urn波長，酶標儀測定各孔od值。
  4. High active phytase producing fungs - aspergillus niger were selected by mutiple uv mutation, the definitions of phytase activity were analysised and the measure wavelength of the enzyme was modified, the factors that influence the preparation of protoplast were investigated. base this, use protoplast - uv mutation and protoplast fusion to filter phytase produce asp. niger

    本文以多輪紫外誘變為主線技術篩選植酸酶的黑麴黴高產菌，分析比較植酸酶酶活定義和植酸酶酶活測定方法並修正其測量波長，考查黑麴黴原生質體制備的影響因素，並在此基礎上，用原生質體紫外復合誘變和原生質體融合技術篩選植酸酶的黑麴黴產生菌。
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