酶的特異性 的英文怎麼說

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酶的特異性 英文
enzyme specificity
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 的 : 4次方是 The fourth power of 2 is direction
  • 特 : Ⅰ形容詞（特殊; 超出一般） particular; special; exceptional; unusual Ⅱ副詞1 （特別） especially; v...
  • 異 : 形容詞1 （有分別; 不相同） different 2 （奇異; 特別） strange; unusual; extraordinary 3 （另外的;...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 特異性 : distinction
  • 特異 : 1 （特別優異） exceptionally good; excellent; superfine2 （特殊） peculiar; distinctive特異功能 s...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  3. Such as when the substrates was benzidine and a - naphthol, the absortion peak was 450nm after catalyticed by dna, and 501nm after catalyticed by pod, if changing substrate to benzidine and pyrocatechol, the absortion peak was 503nm after catalyticed by dna and 603nm after catalyticed by pod. the second difference was the different result after hplc : the result of hplc ( substrates : benzidine and a - naphthol ) showed that the products under the affect of dna and pod had different value on the peak and the area

    然後對以聯苯胺與-萘酚為底物，以h _ 2o _ 2為氧化劑，進行高效液相色譜分析，分析結果表明dna與過氧化物酶作用后的產物在峰面積和峰高上有不同數值；同時，不同形式的dna對該反應都有催化活性，表明只要是生物來源的dna （非特異性dna ）都具有一定程度的催化活性。
  4. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  5. The hatching enzyme from brine shrimp, artemia shrimp, is a pivotal protease which help the encysted embryo escape from its hatching membrane when hatching

    鹵蟲孵化酶（ he ）是由鹵蟲早期胚胎特異性分泌的、在孵化過程中起關鍵作用的一種蛋白酶。
  6. Constructing human colorectal cancer cell line with stably down - regulated grp94 ( 1 ) the plasmid prc / rsv - ribol that contains specific grp94 - targeting ribozyme and the control plasmid prc / rsv were miniprepared, respectively, cleaved by endoenzyme pvuii

    穩定下調grp94的人大腸癌細胞克隆株的構建（ 1 ）分別對含有特異性打靶grp94核酶的質粒prc rsv - ribo1和對照組質粒prc rsv進行小量提取、 pvu酶切鑒定。
  7. The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label

    酶免疫分析技術的質量依賴于抗原的純度、抗體的特異性、合適標記酶的選用，其靈敏度取決于標記酶的高度純化和高轉化率。
  8. This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected

    本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。
  9. Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

    首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
  10. In addition, the heparinase is stimulated in the presence of ca2 + and mn2 +, but is inhibited by cu2 + and fe3 +. neither pmsf nor edta significantly affected enzyme activity. by infrared spectrum and proton resonance spectrum analysis, we found smaller polysaccharides, unsaturated bond and reducing sugar, which are specific products by heparinase

    在產物分析中發現，該酶作用於肝素后，還原糖數量明顯增加，通過紅外色譜及質子譜的分析發現，產物中出現了含不飽和雙鍵的還原寡糖，這是肝素酶作用於肝素后的特異性產物，從而可以進一步證明該菌確實產生肝素酶。
  11. But there are still no reports about the relationships of dnpi - like and gabaergic immunoreactive ( gad - li ) terminals with pag - like immunoreactive ( pag - li ) neurons in " zone - shaped area ". to answer these questions, we observed systemically the synaptic connections among the pv - like immunoreactive neurons, fibers and terminals and the connections between dnpi - like, gabaergic terminals and pag - li neurons using the methods of electron microscopic imrnunohistochemistry, triple - immunofluorescence histochemistry and retrograde tracing method combined with pre - embeded immunoelectron microscopic double - labeled technique

    但是目前對新發現的囊泡膜mu轉運體一dnn樣陽性終末與帶狀區內pag樣陽性神經元之間ej關系，以及谷氨酸脫發酶（ gad ，是gaba能神經元和終末的特異性標識物）是否參與其調控作用，尚缺乏系統的形態學資料。
  12. Glu of cns mainly comes from glutamine and phosphate activated glutaminase ( pag ) can decomposed the glutamine to glu and ammonia and is the main source of the glu of the cns, so pag is used as the special marker of glutaminergic neurons

    磷酸激活的谷氨酞胺酶（ pag ）能催化谷氨酞胺水解為giu和氨，是中樞內gill的主要來源。困此pag一直被作為研究gill能神經元的特異性標識物。
  13. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  14. Biological recognition with enzymes or antigen antibody binding are used to achieve high specificity

    酶的生物識別或抗原抗體結合使光纖傳感器可以獲得高的特異性。
  15. It delivers the false ? folding peptides for presentation to proteasomes in normal cells, while in tumor cells it acts as main tumor - resistant antigen, inducing specific immune response. recent research found that grp94 also relates with cell cycles, differentiation and apoptosis

    此外發現它還具有抗原提呈作用，在正常細胞中參與折疊錯誤蛋白到蛋白酶體的提呈；在腫瘤細胞中作為主要的腫瘤排斥抗原，引發針對該腫瘤細胞的特異性免疫反應。
  16. Chi 1 degraded colloidal chitin both endolytically and exolytically with jv - acetylglucosamine, chitobiose and chitotriose as its products while a c - teminal 302 - amino - acid d eleted mutant had its degrading pattern shift to chitobiose, chitotriose and chitotetraose. further research showed a shifting of the minimal length of chitooligosaccharide needed for these two correlated proteins. in the case of full - length chitinase, it was chitotriose while with its truncated form, it turned out to be chitotetraose

    進而對chi1和chi1 ~ -做了體外表達、純化及功能的比較分析，首次揭示了幾丁質酶潛在的幾丁結合區對酶作用底物與產物的特異性影響，即chi1 ~ -仍具有較強活性，但其膠體幾丁質降解產物為幾丁二、三、四糖。
  17. The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation, deae - sepharose fast flow ion - exchange chromatography, and sephacryl column gel - filtration, and its biochemical and enzymological properties were identified in this study. it was found that the deduced molecular weight of he in sds - page is about 98. 5 kda, and its proteolytic activity was optimized at ph of 7. 5 - 8. 5 and at temperature of 40, respectively

    我們利用67硫酸銨沉澱、 deae - sepharosefastflow陰離子交換柱層析和sephacryl凝膠過濾柱層析，並以酪蛋白為其蛋白酶水解活性的檢測底物、以卵膜為其卵殼裂解活性的特異性底物，從鹵蟲胚胎孵化液中分離純化出了鹵蟲的孵化酶分子，其在sds - page電泳中的分子量約為98 . 5kda 。
  18. Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method

    首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。
  19. Based on the genetic identification of 5 animal drugs and compound recipe in arthropod, 5 parts are studied. they are the total dna extracted of the drugs, the 18s rrna genetic fragment studying of 5 animal drugs, the particular primer identification of 5 drugs, the genetic enzyme cutting identification of 5 drugs and the particular primer identification of the compound recipe

    本文圍繞5種節肢動物及其復方小兒抽風散的dna遺傳標記鑒定，主要進行了5個有關方面的研究，即：商品藥材總dna的提取； 5種動物藥18srrna基因片段的序列研究； 5種藥材的特異性引物pcr鑒定； 5種藥材的基因酶切鑒定； 5種藥材在復方中的特異性鑒定。
  20. The amplified e2 fragments of two hcv strains were all 1280bp in length by 2 % agrose gel electrophoresis. expected size of 1280bp of 2 fragments and their specificity were confirmed by restriction endonuclease digestion and then they were cloned respectively into pmd - 18t vector

    以此病毒rna為模板，利用rt - pcr技術，擴增出了豬瘟野毒株hcv - jn 、 hcv - yc完整的e2基因，用pmd - 18t載體克隆，經電泳檢測、酶切分析及pcr鑒定初步證實了所擴增片段的特異性。
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