酶的誘導 的英文怎麼說
中文拼音 [deyòudǎo]
酶的誘導
英文
enzyme induction- 酶 : 名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
- 的 : 4次方是 The fourth power of 2 is direction
- 誘 : 動詞1. (誘導) guide; lead; induce 2. (使用手段引人隨從自己的意願) lure; seduce; entice
- 導 : 動詞1. (引導) lead; guide 2. (傳導) transmit; conduct 3. (開導) instruct; teach; give guidance to
- 誘導 : guide; lead; induce; guidance; induction
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Effect of active component of four types of chinese herbs on aldose reductase activity and non - enzymatic protein glycation in d - galactose induced rats
半乳糖誘導大鼠醛糖還原酶活性和蛋白非酶糖化的作用In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays
本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體,研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響;然後在得到高頻再生體系的基礎上,通過農桿菌介導法轉化甜菜堿醛脫氫酶( badh )基因,以gus染色組織分析為依據探討了影響轉化效率的各種因素,建立了高效、可重復的基因轉化體系,為四倍體刺槐目的基因的導入打下了基礎。This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan
在里氏木霉rutc - 30的基礎培養基( mandels營養液)中加入固定碳源乳糖和槐豆膠,然後將可變碳源(雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖)進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗,並以槐豆膠為底物用3 , 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基,用該培養基培養的里氏木霉( t . reesei ) rutc - 30使其轉錄的-甘露聚糖酶( - 1 , 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ) mrna量能夠滿足rt - pcr的要求。Gene inductive expression of bifidobacterium on glutathione s - transferase in ht29 cell line
29細胞谷胱甘肽巰基轉移酶基因的誘導表達Induction of inulinase formation in kluyveromyces marxianus by xylose
木糖對馬克斯克魯維酵母菊糖酶合成的誘導作用H2o2 was cytochemically detected in the cells of epidermal hair and the cell wall of vascular parenchyma cell. cat and apx were the two major h2o2 - scavenging enzymes in plants. when seedlings of arabidopsis thaliana were treated by vd - toxin, the activity of cat and cat3 mrna level were decreased after sa treatment
外源sa 、 no供體處理擬南芥幼苗均能誘導h _ 2o _ 2的積累, no供體的誘導作用最強; no合酶抑制劑處理則未表現出h _ 2o _ 2含量的增強: h _ 2o _ 2的積累部位主要在葉片的表皮毛和維管束組織。The carbofuran - degrading experiment of cds - 1 was carried out in lab scale, the results showed that the highest degrading efficiency was obtained with ph, temperature being 7. 0, 30c respectively ; the change of aeration had no influece on degrading rate ; the increasing inocula could accelerate carbofuran degrading progress ; the degrading capability of cds - 1 was n ' t inhibited by high carbofuran concentration ; the addition of low concentration of nutrients had no distinct effect on the degrading rate while high concentration had inhibiting effect the distribution of degrading enzyme was also primarily studied, the results showed that degrading - related enzyme was endocellular and degrading progress was not cometabolism
Cds - 1的降解酶(系)是誘導酶(系) ,存在明顯的誘導期;胞內、胞外酶實驗表明呋喃丹降解酶(系)存在於細胞內。添加低濃度外源營養物質對cds - 1的降解性能無明顯影響,說明cds - 1降解呋喃丹的過程不屬于共代謝過程,可以在無外源營養物質存在的條件下降解呋喃丹。添加高濃度外源營養物質會對該菌降解性能產生抑制。It was found that b - 6 isolated by the method of distinct zone of clearing in cellulose - congo red agar medium combined with measuring the enzyme activity of liquid culture filtrates had comparatively higher cellulase activity. by measuring activity of cellulase of strains growing in medium with different carbon sources and of washed mycelium induced by different carbon sources in certain time, it found that the formation of cellulase was regulated by the nature of the carbon source used for b - 6 and as3. 3711
真菌纖維素酶是一種誘導酶,碳源同時也是主要的誘導物來源,為了研究碳源對真菌纖維素酶合成的誘導機理,本文利用液體生長培養和洗滌菌絲誘導培養法研究了不同碳源對兩菌株的誘導特性,並用電泳分析法研究了不同碳源的誘導酶譜。To study the biologic effects and the underlying mechanism of secretory phospholipase a 2 ( spla 2 ) and ceramide, we firstly investigated the relationship between certain lipid signalingmolecules - mediated signal transduction and the spla - induced cell death
為了研究分泌型磷脂酶a2和神經酰胺對細胞產生的生物學效應及其機制,我們首先探討了某些脂類活性分子介導的信號傳導與分泌型磷脂酶a誘導的細2胞死亡之間的關系。The tissue distribution of monooxygenase activities of cotton bollworm, helicoverpa armigera
氰戊菊酯和苯巴比妥鈉對敏感和抗性棉鈴蟲中腸多功能氧化酶系的誘導作用This article elaborately studied the induction mechanism of different carbon sources to cellulase synthesis of trichoderma sp. ass. 3711 and aspergillus sp. b - 6, evaluated the cellulase - producing property of b - 6 isolated from rubbish compost by contrast to as3. 3711 offered by ccccm, and studied comparatively the physico - chemical properties of cmcase come from as3. 371 land b - 6
本文以從垃圾堆肥中篩選的高產纖維素酶的麴黴b - 6 ( aspergillussp . )菌株和中科院微生物菌種保藏中心ccccm惠贈的木霉as3 . 3711 ( trichodermasp . )菌株為實驗材料,較為詳盡的研究了碳源對兩株真菌纖維素酶合成的誘導調控機理。For the acute exposure duration, there expressed the dose - thresholds and time - thresholds in the effects of cu2 + on the activities of cat, pod and sod in two macrophytes. when the test species were exposed at low dose and for a short period, cu2 + can stimulate activities of antioxidant enzymes. once exceeding the thresholds, cu2 + may inhibit enzymes activities
Cu ~ ( 2 + )低濃度和短時間內對抗氧化酶有誘導作用,超過一定的閾限會產生抑制作用;金魚藻cat 、 pod 、 sod活性在7天內的最大抑制率與暴露cu ~ ( 2 + )濃度呈顯著正相關。After the screening, the expression and identity of the gene for nadp - malic enzyme in leaves of aloe vera l. under salt stress had been done. methods : construction and screening of the subtractive library : to construct a cdna subtractive library of aloe vera l. under salt stress, the leaves of aloe vera l were removed from the seedlings which were treated with 300 mm nacl. suppression subtractive hybridization ( ssh ) was carried out and a subtractive library was constructed
鹽脅迫下庫拉索蘆薈葉子中nadp -蘋果酸酶基因的表達鑒定:為了檢測蘆薈鹽脅迫下庫拉索蘆薈cdna消減又庫的構建篩選及nadp一蘋果酸酶基因的鹽誘導表達中nadp一蘋果酸酶基因『刀叻心)的表達和nadp一蘋果酸酶( nadp一ma1ieenzyme )蛋白的積累是否受著高鹽的誘導,我們利用耐鹽品種庫拉索蘆薈( a了口。And regulation of stem elongation, flower inducement and seeds and fruit development are three very crucial sections in fruit industrial, so it is important to clone the gibberellins biosynthetic enzyme genes and to research on their expressing modes and their regulation roles
而莖的生長,開花的誘導,和果實種子的發育都是果樹生產中控制產量和果實品質非常重要的三個環節,因此克隆赤霉素生物合成酶的基因並研究其表達方式及表達調控在果樹生產上是非常重要的。Based on the principle of sedimentology and the environmental behavior characteristics of heavy metals, the index of geoaccumulation igeo is used to evaluate heavy metal pollution in sediments in the benxi section of the taizi river. the results show that heavy metal pollution in the sediments are serious. the main heavy metal pollutions are copper, lead, zinc, cadmium and mercury
論述了查爾酮合酶基因chs在植物防禦反應中與病原物的相互關系,探討了與查爾酮合酶因誘導表達相關的順式作用元件和反式作用因子及其dna -蛋白質間的相互作用,闡明其在植物抗病性防禦反應中的重要作用。Addition of iptg to growing culture of the lysogen induces t7 rna polymerase, which in turn transcribe the target dna in the plasmid. in the presence of glucose and appropriate conditions such as temperature and concerntration of iptg, a 52kd protein with tryptopanase activity was expressed
摸索發酵條件,如改變培養溫度和iptg濃度等,發現在30培養條件下, 0 . 2mmiptg誘導時,發酵液中的吲哚含量最高,表明低濃度的誘導劑或低溫誘導有利於表達出有活性的色氨酸酶。A. niger m - l which was screened in our laboratory produced a strongly a - transglucosidase. in this paper, studies on the fermentation conditions, purification and characterization of a - transglucosidase and its necessary groups was carried out in this dissertation. the main reports were as following : the fermentation conditions in shaking flasks were investigated by the method of single - factor analysis, the suitable main medium was achieved : which contained 4 % a, 2 % b and 1 % g ; the a. niger m - l was inoculated into 100ml medium in flask, shaking in 33 c at 140r / min for four days, with initial ph6. 5 and 6 % inocula volume ; adding 0. 1 mmol / l methyl a - d - glucopyranoside had inductive effect on enzyme formation, the a - transglucosidase activity amounted to 296. 05u / ml
本研究以黑麴黴m - 1為出發菌株,對其-葡萄糖轉苷酶的產酶影響因素、純化、酶學性質以及必需基團進行系統的研究,結果如下:通過對影響黑麴黴m - 1產-葡萄糖轉苷酶的單因素分析,得液態發酵生產-葡萄糖轉苷酶的最適產酶條件為: 4 a , 2 b和1 g ;在33 ,起始ph值為6 . 5 ,轉速為140r min ,接種量為6 ,裝液量100ml條件下,發酵4 . 0d ,酶活力達296 . 05u ml ,添加0 . 1mmol l的酶作用底物甲基- - d -葡萄糖苷對產酶的誘導作用最大。Expression and identity of the gene for nadp - malic enzyme in leaves of aloe vera l under salt stress : to investigate whether the expression of nadp - me gene and the accumulation of nadp - me protein induced by salt treatment are related to salt tolerance in the aloe plant, northern blot analysis was performed and the activity of nadp - me protein was measured in a tolerant aloe, aloe vera l, and a sensitive aloe, aloe saponarea haw under salt stress
對其中的3個進行單向測序。其中一個序列在genbank中登錄,獲得的序列號為no . ay179511 . 2 .鹽處理12個小時后nadp一蘋果酸酶mrna誘導增加,且兩種蘆薈中的表達量都在48 , j 、時后達到高峰。但是其表達水平是不相同的, a了。Maximal yields of cellulase were obtained on the relatively undegraded cellulose ( for example, a - cellulose and avicel )
結果發現結構較完整的纖維素類碳源對酶的誘導活性優于其它碳源物質。From the result of electrophoresis, it known that the different components of the enzyme system were expressed cooperatively. in order to study the essence of cellualase induction of different carbon sources, the extracellular, plasm - membrane - bound and intracellular cellulases were made to transform different soluble inducers, and the productions were analyzed by gc chromatogram. the results supported the assumption that cellobiose acted as the direct inducer or the metabolic analogue, b - gentiobiose from cellobiose acted as the true inducer through different metabolism ways in different strains
制備細胞膜外、細胞膜、細胞內纖維素酶,用定位於這三部位的纖維素酶分別轉化底物,然後進行氣相色譜定性分析,從而探討了不同碳源之間的誘導本質,結果認為不可溶的胞外纖維素以纖維二糖為橋梁,遵循不同的代謝途徑,直接或間接地誘導了兩株不同真菌纖維素酶的合成。分享友人