酶細胞化學 的英文怎麼說

中文拼音 [xìbāohuàxué]
酶細胞化學 英文
enzyme cytochemistry
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 學 : Ⅰ動詞1 （學習） study; learn 2 （模仿） imitate; mimic Ⅱ名詞1 （學問） learning; knowledge 2 （學...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. The distribution of the brine shrimp hgcs varies greatly from the species studied till now. one hour after hatching, neither the dorsal - anterior area nor the other dorsal area remained positive immunoreactivity signal. and 2 hours after hatching, there was no typical hgcs in the body of the brine shrimp and the remained hatching enzymes may participate in digesting the left vitellin in the nauplius

    鹵蟲hgc最初出現至孵化前1h時均為全身性分佈，從孵出到孵出后2h ，頭鹵蟲孵化酶的生物化學性質及孵化腺細胞的免疫組織化學研究部的孵化酶顆粒已經減少，而變為非全身性分佈，到孵出后sh ，孵化酶顆粒已基本消失殆盡。
  2. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  3. The potential for a wide range of biotransformations using either immobilized cells or extracted plant enzymes undoubtedly exists, the major restriction to development being our lack of fundamental knowledge of the general biochemistry and enzymology of natural product synthesis

    盡管我們在自然產品合成方面的普通生物化學和酶學知識還很匱乏，但使用固定化細胞或植物提取酶的生物轉化得到大范圍應用的可能性無疑是存在的。
  4. In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test

    在第一部分試驗中，首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液，然後經6層非連續percoll梯度離心法（ 15 、 22 、 30 、 40 、 50和60 ）分離，通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量，並以伊紅y排斥試驗測定細胞的存活率。
  5. Methods the influence of fluoride on the rat first passage osteoblast were evaluated by histochemistry, enzymehistochemistry and electron microanalysis in vitro

    方法應用組織化學、酶組織化學和電子顯微分析等手段觀察氟對體外培養的鼠第一代成骨細胞的影晌。
  6. The results of these early research work showed that rna polymerase iii transcription was localized in the nucleoplasm. however, with the development and the application of new technologies since 1990s, the controversy arose on the transcription sites of rna polymerase iii. in recent years, more and more scientists presumed that rna polymerase iii transcription might not occur in the nucleoplasm but in the nucleoli

    自上個世紀八十年代初期，人們相繼運用細胞化學染色、電鏡放射自顯影等進行研究的結果表明： rna聚合酶的轉錄發生在核質中，但隨著新的研究技術的發展和應用，人們卻發現rna聚合酶的轉錄可能發生在核仁中，從而對早期的研究結果提出了質疑。
  7. This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected

    本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。
  8. Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly

    方法：通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型，主要採用抗fos 、膠質原纖維酸性蛋白（ gfap ）和酪氨酸羥化酶（ th ） （或加壓素? vp ）免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法，系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核（ son ）神經元? ast超微結構的變化、延髓內臟帶（ mvz ）和son及下丘腦室旁核（ pvn ）之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。
  9. Elevation of intracellular calcium ions may be partly induced by increased influx through sarcolemma l type - calcium channels. intracellular calcium elevation, on one hand, would activate calpain, a calcium - dependent cysteine protease that degrade the myofibrillar proteins and cause muscle atrophy ; on the other hand, result in activation of calcineurin which enhance the activity of mhc i promoter and inhibit a shift of mhc isoforms from slow to fast in soleus

    這樣，可能使得萎縮比目魚肌細胞內鈣離子水平升高，細胞內鈣離子靜息濃度的增加一方面激活calpain ，增加收縮蛋白的降解，使肌肉萎縮；草四軍醫大月卜祠成士學位論文另一方面通過激活鈣調神經磷酸酶，增加快型mhc基因的表達，使骨骼肌肌球蛋白重鏈（ mhc ）發生由慢型向快型的轉化。
  10. There was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i

    G ） i型膠原、纖維粘連蛋白促進細胞增殖，細胞接種后3 、 6 、 gd三個檢測時間點，實驗組細胞均明顯高於對照組。與1型膠原相比，纖維粘連蛋白刺激作用更強。 ） i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化，不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ，而且堿性磷酸酶活性明顯增高，堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性， i型膠原組mscs細胞堿性磷酸酶活性較fn組更高，有顯著性差異；同時，兔疫組化染色表明，經纖維粘連蛋白作用的mscs1型膠原表達陽性。
  11. Along with the development of the cytobiology and the molecular biology, and thoroughly research of the biophysics, the biochemistry, the genetics and immunology, it has cultivated the modem biological technology, such al genetic engineering, cellular engineering, enzyme engineering, fermentation engineering and so on, to change biology characteristic to carry on the material transformation, has formed the front biological examination technology : the dna probe, the pcr technology, the molecular mark, the bioluminescence technology, genechip technology and so on the widespread application of these advanced biotechnologies in dairy industry baa impelled the dairying technical transformation, and has been having vital significance to dairy production, research and dairy product security

    摘要隨著細胞生物學和分子生物學的發展及對生物物理、生物化學、遺傳學和免疫學研究的深入，培育了基因工程、細胞工程、酶工程、發酵工程等改變生物特性進行物質轉化的現代生物技術，形成了dna探針、 pcr技術、分子標記、生物熒光技術、基因晶元技術等前沿性的生物檢測技術，其在乳品工業中的廣泛應用，推動了乳業的技術變革，對乳品生產、研究和乳品安全意義重大。
  12. Green : the peroxisomes labeled with fitc ; blue : the dna labeled with dapi ; material : rat liver cells

    [何鍵.大鼠肝細胞過氧化物酶體的免疫膠體金標記.中山大學本科生畢業論文. 2005
  13. Microwave ablation of hepatocellular carcinoma : the tumor cell viability determined by enzyme histochemical stain

    微波凝固肝癌細胞活性的酶組織化學檢測
  14. The results showed the technique suit either completely to immunohistochemical study of the pulp matrix and of the cell surface expression that was weaker antigenicity, or to enzyme histochemical staining of the dental pulp

    結果表明，它完全滿足牙髓基質和抗原性較弱的細胞表面標志等成分的免疫組化染色，同時也適用於牙髓的酶組織化學染色。
  15. Lin s x, wang q, wang y l. interactions between escherichia coliarginyl trna sythetase and its substrates, biochemistry. 1988, 27 : 6348

    汪靜英，王應睞.琥珀酸脫氫酶的研究- -琥珀酸脫氫酶還原細胞色素c的性質.生物化學與生物物理學報. 1981 . 13 : 347 - 352
  16. Unicolor and lilium asiatic hybrids or cultivars in lilium asiatic hybrids were researched with their parents by karyotype, soluble protein, esterase isoenzyme and peroxidase isoenzyme. the results provided identification markers of cytology and biochemistry for hybridization at the early stage in lily breeding programs. the cluster analysis according to similarity coefficient of soluble protein and peroxidase showed that " yellow " and " omega " have the most closest relation in lilium asiatic hybrids

    本研究對百合屬幾個植物的親緣關系進行了可溶性蛋白質、過氧化物酶同工酶分析，同時對亞洲百合雜交系內雜交及其與原種系間的雜交後代進行了核型、可溶性蛋白質、過氧化物酶和酯酶同工酶分析，以期為百合屬植物親緣關系分析提供生化依據，以及為雜交後代的早期鑒定提供細胞學、生化水平的檢測指標。
  17. 7 fernandez - rodriguez cm, prieto j, quiroga j, et al. enhanced urinary excretion of cgmp in liver cirrhosis, relationship to hemodynamic changes, neurohormonal activation, and urinary sodium excretion. dig dis sci 1997, 42 ( 7 ) : 1416 ? 1420

    6黃潁秋.促紅細胞生成素與一氧化氮合酶抑制劑對肝硬化大鼠高動力循環狀態的影響.中華醫學雜志， 1998 . ， 78 （ 2 ） ： 139 ? 142
  18. In the laboratory experiment part, human peripheral blood, cultured cells and icr mice were study objects. the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test. the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test. the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry, western blot and rt - pcr

    流行病學結果顯示長期小劑量輻射接觸與染色體不分離呈正相關，為進一步在細胞遺傳學和分子生物學方面研究小劑量電離輻射與染色體不分離關系及其機制，本課題第二部分以外周血、培養細胞、 icr小鼠為研究對象，用外周血染色體計數和單細胞受精卵染色體計數的方法研究小劑量輻射和拓撲異構酶復旦大學2000級博士生學位論文11a抑制劑及其二者的協同效應對有絲分裂和減數分裂染色體不分離的影響，用免疫細胞化學染色、 westernblot 、 rt pcr等方法研究了電離輻射引起拓撲異構酶a表達變化。
  19. Cells were centrifuged for 5min at 1000rpm and removed supernatant. the cell peller was resuspended in 1 ml culture media and divided into two glass flask. cell surface makers ( cd45, cd90 ) were detected by immunocy - tochemistry

    當傳到第五代時，取一瓶mscs用胰酶消化，以8x10vcm 『濃度接種在六孔板中的蓋玻片上，應用免疫細胞化學方法檢測cd45人d90的表達。
  20. The fetal liver stem cells were isolated by collagenase digestion, gravity sedimentation and density gradient centrifugation, identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition

    採用膠原酶消化、重力沉降及密度梯度離心方法分離人胎肝幹細胞，通過免疫細胞化學方法對其進行初步鑒定，以及應用流式細胞儀等對其生長狀況進行評估。
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