酶選擇性 的英文怎麼說
中文拼音 [xuǎnzháixìng]
酶選擇性
英文
enzyme selectivity- 酶 : 名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
- 選 : Ⅰ動詞1. (挑選) select; choose; pick 2. (選舉) elect Ⅱ名詞(挑選出來編在一起的作品) selections; anthology
- 擇 : 擇動詞(挑選) select; pick; choose
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 選擇 : select; choose; opt; election; choice; culling; alternative
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The lipase b from candida antarctica ( cal - b ) displays high enantioselectivity on a broad range of substrates, making it an accepted biocatalyst for asymmetric organic chemistry
摘要從南極假絲酵母中提取的脂肪酶b ,具有較高的對應異構體選擇性,廣泛應用在培養基中,作為不對稱有機化學的生物催化劑。Analysis of copper binding protein by sds - page, three main protein bands observed. the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc. 4
Sds page分析得到3條主要蛋白帶,剪下這三條帶進行膠內賴氨酸內切酶的消化,通過高效液相色譜分離肽段,選擇性進行肽段的氨基酸序列測定。The bacteria - bursting enzymes that caught gasson s attention are called lysins. different lysins attack specific bacteria and the bacteriophage lysins researched by gasson s team can be used to detect or selectively kill listeria and clostridium
不同的細胞溶解酶攻擊特定的細胞,加森小組所研究的噬菌體細胞溶解酶可用於探測或選擇性地殺死李斯特氏菌和梭狀芽孢桿菌。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗,將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合,獲得新抗原- 35肽序列;並在合成、純化後分別與弗氏佐劑、免疫刺激復合物( iscoms )混合后免疫不同遺傳背景的雌性小鼠,觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變:以及在ivf下,新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。Abstract : a novel enzymatic reaction ? ammonolysis was introduced in thenineties. this reaction not only provides a synthetically useful and mild alternative method for the synthesis of amides, but also can be used for the kinetic resolution of chiral alcohols or chiral carboxylic acids. in addition to enantioselective hydrolysis, esterification and transesterification, ammonolysis also shows high enantioselectivity in the resolution of racemates
文摘:酶促氨解反應是20世紀90年代中期發現的一種新型反應,在脂肪酸酰胺的合成、手性藥物(手性酸及手性醇等)的拆分中顯示出巨大的應用潛力,是除立體選擇性水解、酯化、轉酯反應之外的另一具有較大開發應用前景的酶促新型反應。Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced
首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘,然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株;接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增,結果表明其中7個菌株具有nifh基因,這7個菌株的編號依次為: c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。By combining approaches, it is possible to improve lipase enantioselectivity, and much is learnt about the lipase enantioselective catalytic mechanism
這幾種方法的研究不僅使改善催化劑的對映體選擇性成為可能,還有助於深化酶對對映體選擇催化機理的認識。Beyond generating customized catalysts, these studies have helped to elucidate the enzyme ' s structure - function relationship and illuminate its enantioselectivity
這些工程不但生產出了指定的催化劑,還有助於闡明酶的結構作用關系,並且有助於說明這些酶的對應異構體選擇性。This review emphasizes some important aspects for the control of lipase enantioselectivity : medium engineering, substrate engineering, modification of enzyme and directed evolution
文章討論了調控脂肪酶對映體選擇性的幾種方法:反應介質工程、底物分子工程、酶分子修飾和定向進化。But polyadenylation in bacteria needs no specific consensus sequence or there is no such sequence signals found. the sites of polyadenylation of bacterial mrna are diverse, including the 3 ' ends of primary transcripts, the sites of endonucleolytic processing in the 3 ' untranslatd and intercistronic regions, and sites within the coding regions of mrna degradation products
細菌mrna多聚腺苷酸化的位點多種多樣,包括初級轉錄產物的3 』末端, 3 』端非翻譯區和順反子間區的內切酶加工位點及mrna降解產物的編碼區內,其腺苷酸化相對無特異性、無選擇性。For the advantages of glucose biosensors are obvious : high specificity, short time, simplicity and low cost of analysis, etc. one of the developments of glucose biosensor is the third generation amperometric enzyme electrode with direct electron transfer, which is commonly prepared by conducting organic salts and conducting polymers
葡萄糖生物傳感器具有選擇性高、簡便、快速的特點,是檢測葡萄糖濃度最常用的方法。為提高傳感器的性能,一個發展方向是制備直接電子傳遞的第三代電流型葡萄糖生物傳感器。目前把利用導電聚合物或導電有機鹽制備的酶電極叫做直接電子傳遞的生物傳感器。Reversed micellar extraction was relatively new separation technique which could provide high selectivity and make less inactivation of enzyme, thus being highly potential for recovery and purification of bioactive products
摘要反膠束萃取是一項新的分離技術,不僅具有高選擇性,而且不易使酶蛋白失活,在提取純化生物活性物質方面具有巨大的工業應用潛力。The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer, high temperature, ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and ultra - filter membrane
雙胸蚓組織中dna酶的分離純化雙胸蚓組織粗提取液經過選擇性酸變性、選擇性熱變性、硫酸按分段鹽析、 deae一纖維素( de52 )柱層析、超濾膜分級分離后得到一個電泳純的dna酶。A novel aqueous two - phase system can be formed by the mixtures of a polymer and cationicanionic surfactants. such a system can be used as a partitioning system of proteins. in this work, we investigated the formation, phase behavior and protein partitioning in aqueous two - phase systems formed by dodecyltriethylammonium bromide / sodium dodecylsulfate / peg and dodecyltriethylammonium bromide / sodium dodecylsulfate / dextran. the ligands with affinity were attached to the polymers and the affinity partitioning of proteins was investigated. it was shown that the surfactants and polymers are enriched in different phases of aqueous two - phase systems. phase separation are promoted by increasing temperature and adding inorganic salts. different proteins are partitioned in different phases. the selectivity of protein partitioning is increased by adding ligands with affinity
報道了由正負離子表面活性劑與高聚物混合溶液形成的一種可用於蛋白質的分離及分析的新型雙水相萃取體系.研究了正負離子表面活性劑(溴化十二烷基三乙銨/十二烷基硫酸鈉)分別與葡聚糖和聚乙二醇混合雙水相體系的形成規律、相行為及牛血清蛋白和溶菌酶在雙水相體系中的分配.通過在高聚物分子中接上親和配基,研究蛋白質在雙水相體系中的親和分配.結果表明,在該體系中,表面活性劑與高聚物分別富集於不同相中.升高溫度及加入無機鹽均可促進雙水相體系的形成,不同蛋白質可分配于不同的相中.親和配基的引入極大地增強了蛋白質分配的選擇性A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接;用連接產物轉化大腸桿菌jml09感受態細胞,置含amp 、 x - gal和iptg的lb平板上培養12 20小時;挑取白色菌落於選擇性培養基擴大培養,堿裂解法小量提取質粒dna ,並進行酶切分析鑒定,結果獲得整合有prvge基因的重組質粒pugedna ,並與其它prv分離株進行ge基因序列同源性分析。Huperzine a has been shown to be a powerful selective inhibitor of acetylcholinesterase and has attracted widespread attention because of its unique pharmacological profile and low toxicity
石杉鹼甲是一高效、高選擇性乙酰膽鹼酯酶抑制劑,由於其獨特的藥理特徵和低毒性,引起了人們的廣泛注意。Conclusion the selective dissociating effect of the detergent on hdl is extremly important and helps the modified enzymes to selectively reacts to hdl - c
結論:表面活性劑對高密度脂蛋白的選擇性解離作用非常重要,這有助於修飾酶發揮其對高密度脂蛋白膽固醇的反應選擇性。Abstract : huperzine a has been shown to be a powerful selective inhibitor of acetylcholinesterase and has attracted widespread attention because of its unique pharmacological profile and low toxicity
文摘:石杉堿甲是一高效、高選擇性乙酰膽堿酯酶抑制劑,由於其獨特的藥理特徵和低毒性,引起了人們的廣泛注意。Analytical methods using biological materials such as enzymes, receptors and antibodies suffer from a lot of disadvantages, e. g. complex and time - consuming procedures, poor stability and reproducibility although showing a high selectivity with these molecular recognition agents
生物物質如酶、受體、抗體等作為分子識別元件具有很高的選擇性能,但是這些物質往往不易長期保存,且操作穩定性不高。The aflp ( amplified fragment length polymorphism ) technique is based on the selective pcr amplification of restriction fragments from genomic dna digested by restriction enzymes completely
擴增片段長度多態性技術( aflp )是基於選擇性擴增完全酶切消化后的基因組dna片段。分享友人