酸性核蛋白 的英文怎麼說
中文拼音 [suānxìnghédànbái]
酸性核蛋白
英文
acid nuclear protein- 酸 : 酸構詞成分。
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 核 : 核構詞成分。
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 酸性 : [化學] acidity; acidness; acerbic; acidic property酸性材料 acid material; 酸性促進劑 acid acceler...
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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At rest, the heterodimeric rel / nf - k b complex is located in the cytoplasm bound to an inhibitory factor, i k b. upon stimulation, i k b is phosphorylated and degraded, free nf - k b then translocates into the nucleus where it binds to k b site to regulate transcription
靜息時, re皿4bh聚體與抑制蛋白kbs結合,在細胞漿中保持無活性。受到刺激, ikb分子迅速磷酸化並降解,釋放nf兒b轉位入核,通過與kb位點結合調節轉錄。The inclusion bodies of recombinant protein were purified with washing buffer consisting of various urea ( 2mol / l and 4mol / l ) for several times, and then dissolve the fusion protein in the denature buffer using 8mol / l urea as denaturant
用含有2mol l和4mol l尿素的包涵體洗滌液洗滌包涵體,在37條件下,洗去了大部分菌體蛋白及其它核酸物質。用8mol l尿素作為變性劑溶解包涵體,包涵體在8mol l尿素中的溶解性非常好。Whole mount in situ hybridization showed that amphihmgb transcripts were present ubiquitously in the early embryos from zygote to neurula, but they decreased gradually in mesoderm and endoderm and neural tube with development, and became restricted to epidermal cells in the hatched larva. it appear s that hmgb plays a role in the mitosis and differentiation of embryonic cells. another amphioxus cdna, amphiubf80, encoding ubiquitin / ribosomal fused protein s27a, was isolated from the gut cdna library of b. belcheri tsingtauense
S004cdna序列編碼一個156個氨基酸殘基組成的蛋白質,與人的泛素蛋白ubf80具有較高的氨基酸序列同源性( 145 / 156同源) ,且該蛋白具有泛素蛋白ubf80所共有的兩個結構域,即由72個氨基酸組成的泛素介導的蛋白降解結構域和由48個氨基酸組成的核糖體蛋白s27a結構域。Cyclic nucleotide dependent protein kinases
環化核苷酸依賴性蛋白質激酶G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位( 1001個堿基)和4012位? 4863位( 851個堿基)片段進行了反轉錄pcr擴增和序列測定,得到mrv的糖蛋白基因全序列,用dnasisv2 . 5demo分析軟體,與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析,結果表明在同一基因型中, mrv和國際標準攻毒株cvs的同源性最高( 96 . 5 ) ,和中國減毒株ctn的同源性最低( 79 . 8 ) 。Tsarg2 with 1 233 bp length was composed of 6 exons and spaned about 115 kb of genomic dna, the putative protein encoded by this gene was 305 amino acid with a theoretical mass of 34 751 and with no significant homology with any known protein in databases. a kind of nucleoprotein was the most impossible
Tsarg2基因的cdna全長為1233bp ,包含6個外顯子,基因組跨越115kb ,編碼由305個氨基酸組成的、分子量為34751的蛋白質,與已知蛋白質無明顯同源性,其最大可能是一種核蛋白。With the increase of concentration of aqueous extract from peganum multisectum increased, root vigor, the contents of chlorophyll, soluble protein and nucleic acid in roots and shoots of alfalfa seedlings decreased, while the activities of protease and nuclease, the contents of o2 ( superscript - ), h2o2 and malondiadehyde ( mda ) increased, the activities of superoxide dismutase ( sod ), catalase ( cat ) and peroxidase ( pod ) first increased and then decreased
幼苗根系活力和葉綠素、可溶性蛋白質、核酸含量隨水浸液濃度的提高而降低,蛋白酶和核糖核酸酶活性及超氧陰離子( o2 (上標- ) ) 、 h2o2和丙二醛( mda )含量則增加,超氧化物歧化酶( sod ) 、過氧化氫酶( cat )和過氧化物酶( pod )活性呈先升后降變化。Ros damage nucleic acid, protein and membrane
活性氧對核酸、蛋白、膜造成傷害。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗,將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合,獲得新抗原- 35肽序列;並在合成、純化後分別與弗氏佐劑、免疫刺激復合物( iscoms )混合后免疫不同遺傳背景的雌性小鼠,觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變:以及在ivf下,新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。Effect of exogenous spermine on proteinase and rnase in wheat seedling under water stress
外源精胺對水分脅迫下小麥幼苗蛋白酶和核糖核酸酶活性的影響The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination, results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm. by indirect fluorescent antibody test mdj - 01 strain was identified with tbev. a part of region encoding e protein was expanded by rt - pcr and sequenced. the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10, respectively, which were 95 %, 94 %. mdj - 01 strain was identified with tbev again
應用間接免疫熒光試驗進行血清學鑒定,結果表明mdj - 01株為tbev 。通過rt - pcr技術擴增部分e蛋白序列並測序,在genbank上進行同源性比較,發現mdj - 01株和森張株與tbevoshima5 - 10株的同源性最高,分別為94 、 95 ,從分子生物學水平上進一步證明mdj - 01株病毒為tbev 。在鑒定的基礎上,本實驗對兩株病毒進行了核苷酸全序列測定。Gene sequence analysis showed that the fragment we have obtained has 94 % and 90 % homology with common buckwheat storage protein and legumins, respectively. meanwhile, by analyzing the amino acid sequence, it shares 93 % - 83 % homology with legumins from common buckwheat and sword bean
採用上述方法獲得的tb22kda核苷酸序列與甜蕎過敏性貯藏蛋白及豆球類蛋白的同源性分別達到94和90 ;氨基酸序列與來自甜蕎中的球蛋白,刀豆蛋白有93 - 83同源性。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法:通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型,主要採用抗fos 、膠質原纖維酸性蛋白( gfap )和酪氨酸羥化酶( th ) (或加壓素? vp )免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法,系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核( son )神經元? ast超微結構的變化、延髓內臟帶( mvz )和son及下丘腦室旁核( pvn )之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。Rt - pcr was used to amplify the cdna of the genome. these cdna fragments were cloned into the plasmid pucm - t. the result indicated that the seguence of the genome was obtained. the genome of aev - nh937 composed of 7055 nucleotides, potentially encodes a polyprotein of 2134 amino acids. the genome of aev - nh937 has 94. 3 % nucleotides identity with the calnek vaccine strain of aev
把它們分段克隆在pucm - t載體上,經序列分析,獲得了aev - nh937毒株的基因組全序列及推導的氨基酸序列,基因組全長為7055個核苷酸,與calnek疫苗株具有94 . 3的同源性,編碼一個含2134個氨基酸的多聚蛋白。Results show that vp37 protein can bind single strand nucleic acid cooperatively and nonspecificallty, and the vp37 - ssrna complex was stable at high salt concentrations, suggesting vp37 is a possible mp. vp37 is the only protein characterized so far showing rna - binding ability in genus fabavirus
為了驗證vp37是否具有核酸結合能力,我們利用6his - vp37蛋白進行了核酸結合實驗,結果表明vp37是一種能非特異性結合單鏈核酸的蛋白;其在結合核酸時具有協同性; na ~ +的變化對其核酸結合能力影響較小。Especially, apoptin is partially similar with ribonucleotide reductase, uridylate kinase and phosphotriesterase in the amino acid sequence. the similarity between them indicates that apoptin maybe takes part in the signal pathway of apoptosis with phosphotriesterase activity or operates on hnrna with ribonucleotide reductase or uridylate kinase activity
凋亡素與它們的長序列相似性表明,它可能具有磷脂酶活性,在細胞信號通路中起作用;或者具有核糖核苦酸還原酶和尿苦酸激酶活性而對hnrna發揮作用;或者是與其中具有某種活性的蛋白質存在競爭性抑制作用。This course includes : the understanding of biomolecules ; the difference between prokaryotic and eukaryotic cell ; the structure of amino acids and their characteristics ; the stereo - structure of protein ; cell membrane and lipids ; the activity of enzyme ; the activity of nucleic acid ; the application of biotechnology
本課程主要包括有生物分子之了解,真核與原核生物之異同;胺基酸之結構及性質;蛋白質與其空間構型;脂質及細胞膜;酵素之作用;核酸之作用;生物技術之應用。It has been shown that domain ia is responsible for cell recognition, domain ii is to be involved in translocation of the toxin across membranes, and domain hi catalyzes the adp - ribosylation of elongation factor2, which arrests protein synthesis and results in cell death
人組蛋白h3是堿性核蛋白,富含精氨酸,在生理條件下, h3的精氨酸帶正電荷,而dna的磷酸基團帶負電荷,所以組蛋白和dna分子主要依靠靜電引力相結合。Tissue sections from every animal were double - labeled with the antibodies of map - 2, cox - 2, gdnf, caspase - 3 and either the neuron - specific antibody neuronal nuclear protein ( neun ) or the astroglial - specific marker glial fibrillary acidic protein ( gfap ). we carried out a series of research to explore the effects and mechanism of map - 2, cox - 2, gdnf, caspase - 3 during tbi and trie d to provide some useful theory basis for both the treatment of tbi in the practice and forensic medicine
並通過上述指標分別與神經元特異性標志物神經元核蛋白( neuronalnuclearprotein , neun )和星形膠質細胞特異標志物膠質纖維酸性蛋白( glialfibrillaryacidicprotein , gfap )進行免疫組織化學雙染色,探討腦損傷后神經元及神經膠質細胞反應性變化情況及其分子生物學機制,以期為腦損傷研究提供有益的數據材料,也為以上指標在法醫學實際檢案的應用提供必需理論依據。To make clear the hypothesis, a middle cerebral artery occlusion ( mcao ) and hypoxia and glucose - deprivation ( hgd ) ischemic models were used in in vivo and in vitro study, respectively. we first studied the cellular localization of kvl. 2 and the co - localization of kvl. 2 protein and vegf receptors flk - 1 and flt - 1, observed the effect of mcao on kvl. 2 expression and phosphrylation in the rat brain in vivo, then investigated the effect of vegf on ischemia / hypoxia cell damage and tyrosine phosphorylation of kvl. 2 in sh - sy5y cells. finally, in order to further elucidate the relationship between vegf ' s neuroprotection and its regulation on kvl. 2 phosphorylation, we used a specific antisense oligodeoxynucleotide ( odn ) to knockdown the expression of endogenous vegf to observe its role in ischemia / hypoxia cell damage and regulation of kvl. 2 phosphorylation
為了驗證上述假設,本文分別在整體和離體水平,採用大腦中動脈缺血( middlecerebralarteryocclusion , mcao )和體外氧?糖剝奪( hypoxiaandglucose - deprivation , hgd )缺血模型,首先了解了kv1 . 2蛋白的細胞定位及與vegf受體flk - 1和flt - 1的共存情況,觀察了整體mcao后缺血再灌不同時間大鼠腦內kv1 . 2蛋白的磷酸化水平變化,然後通過外源性給予vegf蛋白,在sh - sy5y細胞株上觀察其對缺血細胞存活率及kv1 . 2蛋白磷酸化水平的影響,最後利用vegf反義脫氧寡核苷酸( oligodeoxynucleotide , odn )特異阻斷內源性vegf蛋白的表達,觀察內源性vegf蛋白在缺血細胞損傷及調節kv1 . 2蛋白磷酸化中的作用,以進一步明確vegf缺血保護效應與其調節kv1 . 2蛋白磷酸化之間的關系。分享友人