重篩 的英文怎麼說

中文拼音 [zhòngshāi]
重篩 英文
resieve
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 篩 : 名詞[書面語] （植物名） sedge
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Movement analysis for the barycenter offset self - in - phase vibration screen

    重心偏移式自同步振動篩的運動分析
  3. Third filter top - quality full groundnuts : when selecting materials, arawana groundnut oil deliberately adjusts the librating boult 2mm wider than usual, only select big and full groundnuts for materials

    第三重保證篩選優質飽滿花生原料：金魚花生油在選取原料上，特意將振動篩調大2mm ，只選取大顆飽滿的花生，符合要求的尺寸，才篩選來作榨油原料。
  4. Check the state of the straw separated : if the straw is seriously broken, the cylinder and the concave must be adjusted to lessen excessive threshing ; if the straw is complete and unbroken, the quantity of wind shall be increased to make the chaff on the sieve surface the state of floating or the opening of the chaffer shall be slightly increased to make the grain fall through the sieve

    檢查單個稻草的狀況：如果稻草嚴重受損，必須調節滾筒和凹面以減小脫粒過猛；如果稻草完好無損，則必須增大風量使得谷篩表面的廢渣飄浮起來，或者慢慢加大谷篩孔，使得穀物穿過谷篩。
  5. Two spider silk protein genes ( avfl and avf2 ) screened from the library are characterized in a lot of repetitive motifs, a high guanosines or cytidines content, a strong preference for adenosine or thymidine in the third position of a codon and rich residues of glycines or alanines in the proteins translated

    試驗結果，該文庫容量為4 . 9 10 ~ 6 。從文庫中篩選到avf1和avf2蛛絲蛋白新基因，具有典型重復序列多， g c含量高，密碼子第三個堿基偏愛使用a t及編碼蛋白中含有大量gly和ala殘基等特點。
  6. According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified

    以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。
  7. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  8. The newly molted 5th instar larva of silkworm bombyx mori was infected with the recombinant virus

    另外，通過共轉染和篩選純化獲得了攜帶appa基因的重組家蠶桿狀病毒。
  9. Selection maskers are the important ingredient of the vector, and it is also a hot research spot of the genetics of lactic acid bacteria at present

    篩選標記是載體的重要組成部分，也是目前乳酸菌遺傳學研究領域的一個熱點。
  10. The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )

    以抗h3n2流感病毒的多克隆抗體（ 100ng l ）包被酶標板，加入制備好的肽庫，用tbst洗去非特異結合的噬菌體，加0 . 2mol l甘氨酸-鹽酸（ ph2 . 2 ） ，室溫放置10min以洗脫特異結合的噬菌體， 2mol ltris - hcl （ ph9 . 1 ）中和后，取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定，其餘噬菌體擴增後用于下一輪篩選，共重復3輪淘洗。
  11. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  12. The document will be retried in a single - threaded filter

    將在單線程篩選器中重試此文檔。
  13. Improved hand picking technology greatly enhance the mineral processing tate of waste in many dressing plants. the flotation in normal temperature is a big breakthrough in mineral processing technology of scheelite. improved jig sizing applied in roughing and grqvity dressing, and improved tungsten fine deposit recovering technology enhance the recovery of tungsten fine deposit

    手選工藝的改進，使各選廠的廢石選出率得到大幅度地提高；常溫浮選是白鎢選礦工藝的重大技術突破；推廣並改進動篩跳汰使這在粗、重選中獲得應用；鎢細泥工藝的改進提高了鎢細泥的回收率。
  14. A cube - shaped rock plucked out of his sifters turned out to be a 4. 38 - carat, tea - colored diamond

    一塊從篩子上拔出的方形石頭原來是一塊重4 . 38克拉（ 0 . 876克）的茶色鉆石。
  15. This paper analysis the data mining of the single nd multiple streams time series, and draw a conclusion that the relationship between the events of the multiple streams time series are the association patterns dependency patterns, sudden patterns, this paper call them are structure patterns, the existing algorithm have n ' t discuss these patterns, although msdd discussed the dependency patterns, however, it ignored the association patterns, sudden patterns, this paper have a definition of the association patterns, sudden patterns and dependency patterns, and have a complete, frank algorithm called twma ( time window moving and filtering algorithm ), the peculiarity of this algorithm is that events is listed by the time window, by this way, the relationship of the events is clear

    本文將它們統稱為結構模式，而這正是目前其它演算法、沒有考慮到的，雖然msdd考慮了事件之間的依賴關系，但它忽略了突變模式，關聯模式等重要的知識表示。本文給出了關聯模式、依賴模式、突變模式的定義，提出了一個比較靈活全面、直觀的挖掘它們的演算法：時間窗口移動篩選演算法twma （ timewindowmovingandfilteringalgorithm ） 。該演算法的一個突出特點是將時間序列事件按時間窗口序列化，使得事件之間的時間關系表示很直觀，該演算法能成功地從多流時間序列中發現了事件之間的關系。
  16. Meanwhile, in order to improve the e. coli with ability of using sugar. we have recombined the vecter of parg25 a which have ability of using sugar with the gene of sucrase length of 7800bp

    同時，為了促進所構建的大腸桿菌具有蔗糖利用能力，我們構建了含有大小為7 . 8kb的蔗糖水解酶基因sacc重組質粒，篩選得到含有兩種基因的重組子，並轉化大腸桿菌，使大腸桿菌獲得蔗糖利用能力。
  17. This paper summarized the main endpoints and special sensitive indices of fish in the study of endocrine disruption, and introduced the main biological characteristics of several fish species such as chinese rare minnow, zebrafish, medaka, guppy, swordtail fish and fathead minnow, and their use in screening, detecting and assessment for endocrine disruptors

    本文主要綜述了利用魚類進行內分泌干擾研究的主要測試終點和敏感效應指標；重點介紹了稀有鮑鯽、斑馬魚、日本青? 、孔雀魚、劍尾魚、黑頭軟口鰷等魚類的主要生物學特性，以及利用它們進行內分泌干擾物的篩選與測試方面的研究成果。
  18. Screening separates much of the heavier trash by use of different-sized screens.

    許多較重的雜質，經由各種不同的篩子篩除。
  19. If trilinear filtering is requested for a texture involved in multitexture blending operations and validation fails, turn off trilinear filtering and revalidate

    如果請求對涉及多紋理混合操作的紋理進行三線性篩選，並且驗證失敗，請關閉三線性篩選，然後重新驗證。
  20. Provide a string array of expressions to apply multiple filters at once

    使用表達式字元串數組可以實現一次完成多重篩選的效果。
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