重組子 的英文怎麼說

中文拼音 [zhòngzǔzi]
重組子 英文
recon
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 重組 : bpr
  1. Meanwhile, in order to improve the e. coli with ability of using sugar. we have recombined the vecter of parg25 a which have ability of using sugar with the gene of sucrase length of 7800bp

    同時，為了促進所構建的大腸桿菌具有蔗糖利用能力，我們構建了含有大小為7 . 8kb的蔗糖水解酶基因sacc重組質粒，篩選得到含有兩種基因的重組子，並轉化大腸桿菌，使大腸桿菌獲得蔗糖利用能力。
  2. After the protein was electrophoresised and purified, the protein activity was detected by elisa, the protein activity of vp1 is higher than vp0 vp3. at last, the activity of vp1 made in our lab was detected with the agentia made in our lab

    將陽性重組子轉化到大腸桿菌er2566細菌內，用ipig進行誘導表達蛋白，蛋白經電泳、純化，然後用elisa方法檢測蛋白活性， vp1蛋白活性相對高於vp0 、 vp3 。
  3. The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli

    從轉化的平板中篩選出陽性重組子，進行不同iptg濃度和不同誘導時間的表達研究，並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ （ 12 ）在大腸桿菌e
  4. The yield reached 10 - 12mg / l. dr. chen ding hu in plant virus group of institute of plant protection of the caas ( chinese academy of agriculture sciences ) selected pap muts recombinants and wanted to produce pap through fermentation to prevention and cure plant virus disease

    中國農科院植保所植物病毒實驗室陳定虎博士成功的篩選到p . pastorispap利用甲醇緩慢型重組子（ mut ~ s ） ，希望能利用微生物發酵的方法來大量生產pap ，將其應用於植物病毒病的防治。
  5. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  6. These large populations facilitate the detection of rare mutational or recombinational events.

    這樣的大群體有利於檢出稀有的突變或重組子。
  7. Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis

    分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。
  8. As a result of corporate re - structuring, which included reorganizing our affiliates and cutting more than 3000 jobs, our earning power has improved

    公司經過重組，包括重組子公司，裁減員工300多個，結果我們的盈利能力提高了。
  9. In this paper, we constructed the genomic dna library of nephila clavipes with the vector supercos 1 cosmid

    以dig - oligo2為探針，菌落原位雜交篩選cosmid文庫，得到56個陽性重組子。
  10. In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia

    並以已構建的載體ptyb2 - mae為模板，通過pcr擴增出融合基因mae - imein - cbd ，將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  11. The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis

    取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。
  12. Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained

    Pcr產物經回收后，經瓊脂糖凝膠電泳鑒定后，與pcambia1303連接並轉化大腸桿菌dh5a ，陽性重組子經pcr和限制性內切酶酶切圖譜分析，表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。
  13. We sequence the inserted gene fragment of the indentified recombinant clone. the result is : angiostatin gene orf ( open reading frame ) links with the orf in expression vector correctly. but the first base of the codon aaa coding for lys414 in plg kringle 4 domain mutates from a to g which leads lys change to glu

    隨后取通過上述鑒定的重組克隆菌，對重組子插入片段測序，結果為： as基因開放讀碼框與表達載體的讀碼框正確匹配相連，但在其kringle4區相當于編碼plg的lys ~ （ 414 ）密碼子aaa的第一位堿基由a突變為g ，導致相應的氨基酸殘基突變為glu 。
  14. Segmentation and reassembly

    分段和重組子層
  15. Construction of recombinant pichia pastoris containing cdna encoding mature peptide of human bone morphgenetic protein - 7 bmp

    7成熟肽畢赤酵母表達重組子的構建
  16. Using rh 109 - 2 as original strain, we investigated the biological characteristics of fusion cells

    最後，以rh109 - 2為出發菌株，對融合重組子進行了生物學特性的考察。
  17. This cdna sequence has been accepted by genbank as hpd - 3 cloned from chinese. the number is af516673

    Pqe一80l / dhfr重組子經bamhi十kpnl雙酶切后，可見大小約為550bp的片斷。
  18. At last, hau3r gene was inserted downstream of ptipa of pij6021, a streptomyces expression vector

    將hau3 ~ r基因克隆到鏈黴菌表達載體pij6021的ptipa下游，獲得重組子phz2085 。
  19. Finally, the forward and backward subtracted cdna libraries including 863 and 360 clones respectively were obtained

    最後所獲正向差減文庫含863個重組子，反向差減文庫含360個重組子。
  20. According to the result it is sure that pa7 also has promoter activity in pseudomonas pseudoaligenes. pa7 - 2 was also proved having promoter activity in the same way

    將次克隆的得到的重組子ppa7 ? 2也以同樣的方法驗證了其在類產堿假單胞菌中的啟動子功能。
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