重組體基因組 的英文怎麼說
中文拼音 [zhòngzǔtǐjīyīnzǔ]
重組體基因組
英文
recombinant genome-
The complete nucleotide sequence of the mitochondrial genome of f. limnocharis was detailedly compared with those of 5 other amphibians. the nucleotide sequences of 22 trna encoded by 6 amphibians mitochondrial genomes were combined and aligned to the homologous sequences of the 11 veterbrate taxa. using teleosts as outgroup, the phylogenetic analyses results show that mp, nj and ml trees all strongly support the monophyly of living amphibians with respect to other living tetrapods and favor a sister group relationship for caecilians and salamanders
我們在測定了澤蛙線粒體全基因組序列的基礎上，與已知其它的5種兩棲類進行詳細的比較分析，同時選擇了11種高等脊椎動物的線粒體全基因序列，以硬骨魚類做外群，用22個trna基因合併數據進行系統發生重建分析，結果表明mp 、 nj和ml樹都強力地支持現生兩棲類動物為單系群並且蠑螈類和蚓螈類為姐妹群關系（自引導值分別為92 、 99 、 100 ） 。The trend is refered to combine the evidence from several genes representing both nuclear and organella genomes with morphology, ontogeny and life history for systematic studies ; while studies at the population level by applying molecular techniques with high differenciation lead an important direction
指出同時應用能夠代表核和細胞器基因組的多個基因結合形態、個體發育和生活史進行系統學分析是其必然發展趨勢；而應用高解析度的分子手段進行居群研究是一個重要的發展方向。Preparation and identification of recombinant adenoviral vectors containing human wild type spk and its mutant gene
攜帶人鞘氨醇激酶及突變體基因重組腺病毒載體的制備及表達Research topics cover the basic and applied parasitology, including the major parasitic groups, pathogenic biology, host specificity, epidemiology, ecology, the control of parasitic infections, and biochemical and molecular parasitology, and ranging from host - parasite relationships of intrinsic biological interest to those of social and economic importance in human and veterinary medicine and agriculture
在整體和分子水平上，應用現代生物技術對危害人體和重要經濟動植物的主要寄生蟲（原蟲類、吸蟲類、絳蟲類、動物線蟲類、植物線蟲類及醫學昆蟲等）和由其引起的病蟲害進行研究，在病原生物學（包括生活史、媒介種類、基因組結構與表達調控等） 、流行病學、生態學，寄生動物與宿主間的相互作用關系等研究基礎上，實現對病原物的診斷、治療和控制。Rearrangements have been occurred in at least twelve mitochondrial genes, which have drastically altered the gene order of the mitten crab e. japonica sinensis to the putative ancestral pancrustacean ( crustacean / insecta ) gene order
中華絨螯蟹線粒體基因組與推測的泛甲殼類原始的線粒體基因順序相比，至少有12個基因發生了重排，造成線粒體基因排列順序顯著的變化。According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene
因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。The 496 bp fragment of the orf of p22 gene and a 561 bp fragment were amplified from the genomic dna of zs strains of toxoplasma gondii. both 496 bp and 561 bp fragments were successfully cloned into the plasmid pthiohisa, b, c and pbudce 4. 1 respectively. 2
從弓形蟲zs株基因組中擴增出p22編碼基因的一長496bp ，另一長561bp的片段，並成功構建含p22編碼基因的原核質粒重組體pthiohisa ， b ， c / p22 ，及真核重組表達質粒pbudce4 . 1 / p22 。This paper describes several latest industrial microbial technologies in detail, which are the synthesis of the chiral diols by epoxide hydrolase from microbie, cofactors regeneration for redox with fdh, production of nano / micro wire by the phage display, metabolic network rebuilding for conventional fermentation and the application of the organic solvent tolerance and the metagenomics technology
本文綜述了幾項最新的工業微生物技術，主要包括：微生物環氧化水解酶催化合成手性二醇、微生物甲酸脫氫酶用於再生氧化還原反應的輔因子、通過噬菌體展示技術得到納米級金屬絲、代謝網路改造和重建用於傳統發酵生產以及有機溶劑耐受菌和宏基因組技術的應用。The biologic toxins produced by bacteria and virus have important effects to organic metabolism and reproduction. the study on bacterial toxin at molecular level, especially, on complete nucleotide sequence determination of pathogenic micro - organism has make it possible to comprehend pathogenic micro - organism pathogenesis and its rule. recently complete nucleotide sequences of near ten bacteria have been examined
細菌、病毒等所產生的生物性毒物對機體的代謝、繁殖機能有著重要的影響，目前對細菌毒素的研究比較透徹，已經上升到分子水平，特別是通過病原微生物全基因組序列的測定，使人們從更高層次上把握病原微生物的致病機理及其規律成為可能。Finally, a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv. the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes. based on the expression of lacz gene, recombinant prv were selected and purified by blue - colored virus plaque
利用脂質體介導的方法，將pltk - ha與prvbartha - k61基因組共轉染于亞單層vero細胞，依據報告基因lacz在細胞中的表達，篩選藍色蝕斑的重組病毒，經數次蝕斑純化后， pcr鑒定、 western - blot分析。Companioning the case of the national blue - star group companion being listed companion by shell of the chemical equipment of south - west listed companion which i did myself as a main charge, first this article will discuss the main factors of selecting shell companion and the patterns of exchanging stock and t heir price. the second this article will discuss the patterns of reconstructing the enterprise ' s assets and some important problems such as the growth and high profits in the recent times. the third, this article will discuss the ways of reconstructing of debts and their flavor. the forth, this article will discuss the effect of the government in the assets reconstructing of listed companions
本文以資本運營和資產重組的基本理論為基礎，結合本人親身參與的藍星科技買殼西南化機間接上市的實際運作經驗及體會，對買殼上市中涉及的目標公司選擇、股權收購方式以及資產置換、債務重組等關鍵性問題和難點問題進行分析，對影響買殼方買殼上市的目的實現的因素進行了論述，針對該案例中存在的具體問題和我國目前殼資源重組中存在的普遍性問題，提出了解決的辦法和思路，結合案例對政府在殼資源重組中應該發揮的作用進行了探討。A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss
採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段，將此基因片段克隆至表達載體pet36b （ + ）上，與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。This paper discusses on some important progresses obtained in the field of life science such as the newly developed pharmaceutics making people rejuvenated, the first case that the mammals give birth to its own clonal baby, the reproductive biological techniques with the characteristics of ooplasm substitution, the research on the measurement of human genome sequence, the activation of melanocyte changing the white hairs into the black ones, and the research on rice cytology, etc
論述了使人變得年輕的制劑、世界第一例哺乳動物生下自己的克隆體、以卵漿置換為特徵的生殖生物學技術、人類基因組測序研究、激活黑素細胞使白發變黑發以及水稻細胞學研究取得的重大進展，並闡述了其生物學意義和應用價值。Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。It was well known that studies on evolutions and taxa of species have been more and more fashionable by means of either some encoding and non - encoding genes of cpdna and nrdna or repeat sequences, its, and multiple genes families of nrdna
利用葉綠體基因組、核基因組中的一些編碼和非編碼基因，以及核基因組中的一些重復序列、基因間隔區和多基因家族等來進行物種演化分類的研究越來越多。Moreover, we also explore the mechanisms of gene rearrangements and taxonomic status of the rice frog. basing on the characteristics of the morphology and mitochondrial genome, we agree with the opinion of separating f. limnocharis from original genus rana
另外還探討了線粒體基因重排機制和澤蛙的分類地位，根據形態學和線粒體基因組的特點，我們認為將澤蛙從傳統蛙屬rana中分出的觀點是合理的。An expression vector with fragment 9 in antisense orientation was constructed to block the expression of the relevant gene ( fragment 9 related gene, fnr gene ) in vero cells. interestingly, we found that the nontargeted mutation frequency induced by mnng was increased significantly, implicating that the product of the blocked gene may be involved in the inhibition of nontargeted mutation
利用反義核酸技術構建含反向插入9號片段的真核細胞表達重組體並轉染細胞，以獲得反義rna阻斷vero細胞中相應基因的表達，發現mn 』 ng誘發的非定標性突變頻率顯著增高，提示被阻斷的相關基因的表達產物可能參與抑制非定標性突變的發生。Compared with nucleic genome, the chloroplast genome is very small and easily to be manipulated genetically, and the foreign gene is site - direct integrated into the chloroplast genome through homologous recombination
與核轉化相比，葉綠體基因組小，遺傳操作簡單，外源基因是通過同源重組機制定點整合進葉綠體基因組。Two bifunctional streptomyces - e. coli vectors were constructed that contained the phage lambda promoter ( pr ) upstream of the his6 - tagged recombinant pks gene
構建了兩個鏈黴菌-大腸桿菌雙功能pks表達質粒，在重組pks基因上游攜帶有噬菌體啟動子。However, with the mtdna sequences have been increasingly determined for vertebrate genomes, the rearranged and repeated genes had been found in many taxa. based on the complete mitochondrial genome
在我們測定的澤蛙線粒體基因組基礎上，通過研究genbank中200多種脊椎動物的線粒體全基因中發生基因重排的類群，總結出脊椎動物線粒體基因重排的特點。分享友人