鏈接片段 的英文怎麼說

中文拼音 [liànjiēpiānduàn]
鏈接片段 英文
link slices
  • 鏈 : Ⅰ名詞1. （鏈子） chain Ⅱ動詞（用鏈栓住） chain; enchain Ⅲ量詞（計量海洋上距離的長度單位） cable length
  • 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
  • 片 : 片構詞成分。
  • 段 : Ⅰ量詞（部分） section; segment; part; paragraph; passage Ⅱ名詞（姓氏） a surname
  • 鏈接 : interlinkage; interlinking; [自動化] catenate;catenation; chaining
  • 片段 : part; passage; fragment; extract; segment; bit; episode; snatch; section
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively

    以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。
  3. Firstly, the major ampullate glands were got from araneus ventricousus. total rna and mrna of ampullate glands were isolated and purified. double strands cdnas were synthesized in the assistance of amv - rt and e. coli dna polymerase i etc. by reverse transcription and replacement synthesis

    首先剝離大腹園蛛主壺腹腺，提取總rna和分離純化mrna ，反轉錄合成cdna ，凝膠層析除去小於400bp片段，並在雙鏈cdna兩端引入ecor的銜接頭，對其進行磷酸化處理。
  4. Then, the plasmid was transformed into jm109. the full length pstvd cdna recombinant plasmid was further identified by restriction mapping analysis and the nucleotide sequence of cdna cloned in pmd 18 - t vector was analyzed with ab1 377 dna sequence. test showed that the cdna of this chinese pstvd isolate was the same as pstvd - kf - 6 mild " type " isolate which originated from naturally infected potatoes in field of cornell university potato breeding program

    利用rt - pgr技術，設計一對引物，對田間採集的經鑒定含pstvd的陽性樣品進行全序列擴增，並將所得產物純化回收，連接到pmd18t - vector中，並轉化至大腸桿菌jm109中，挑選白斑進行雙酶切（ saci / ecorv ）鑒定證明插入片段為359bp大小，進行序列測定，所得克隆基因為pstvd全序列負鏈，大小為359bp 。
  5. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  6. A 1. 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e. coli / streptomyces shuttle vector with conjugation function ( containing orit gene ). as a result of above procedures, a recombinant plasmid pid03 was obtained

    將1 . 5kb的安普黴素抗性基因片段插入到aved基因中的nrui酶切位點，再將此滅活的aved基因片段插入到具有接合轉移功能（含有orit基因）的鏈黴菌?大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pid03 。
  7. A fragment, containing 2. 0kb cloned 5. tenebrarius dna and reported genes of erme and xyle, was inserted in plasmid phz132 ( an e. coli - streptomyces shuttle plasmid incorporating orit from rk2 ) to construct disruption plasmid pzxb0l4. the plasmid was transformed into e. coli et12567 ( puz8002 ) to construct recombinant e. coli et12867 ( puz8002, pzxb014 )

    將克隆到的鏈黴菌dna2 . 0kb片段以及報告基因erme 、 xyle插入到具有orit的大腸桿菌?鏈黴菌穿梭質粒phz132中構建接合轉移質粒pzxb014 ，並將其轉入大腸桿菌et12567 （ puz8002 ）中，獲得供體菌et12567 （ puz8002 ， pzxb014 ） 。
  8. An internal segment of the whig gene of s. griseus was amplified from plzl through pcr. the 304bp dna fragment was inserted into the ecori / bamhi site of e. coli - streptomyces shuttle plasmid pkc1139, generating pkc1139 : : a whig, named plz107, for gene disruption

    Pcr法克隆whig基因內部304bp片段，連接到大腸桿菌-鏈黴菌穿梭質粒嚇0139 ，構建了基因陽壞用重組質粒賊q139 ： ：凸mg ，命名plz107 。
  9. Of course, you want to be sure that the schema snippets in the wsdl contain semantic links, but you also want to try to sprinkle semantic links into other parts of the description as well

    當然，您希望wsdl的模式片段中包含語義鏈接，但是也希望把語義鏈接應用到描述的其他部分。
  10. S. tenebrarius chromosomal dna, partially digested with sau3al to yield fragments of 6 ~ 15kb, was ligated with vector pij702

    提取黑暗鏈黴菌h6總dna ，經sau3ai不完全酶切、凝膠電泳，收集6 15kb片段連接到載體pij702 。
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