限制內切酶 的英文怎麼說

中文拼音 [xiànzhìnèiqiē]
限制內切酶 英文
restriction enzyme
  • 限 : Ⅰ名詞（指定的范圍; 限度） limit; bounds Ⅱ動詞（指定范圍, 不許超過） set a limit; limit; restrict
  • 制 : Ⅰ動詞1 （製造） make; manufacture 2 （擬訂; 規定） draw up; establish 3 （用強力約束; 限定; 管束...
  • 內 : 名詞1. （內部; 里頭; 里邊） inner; inside; within 2. （妻子或妻子的親屬） one's wife or her relatives 3. （姓氏） a surname
  • 切 : 切Ⅰ動詞1 （合; 符合） correspond to; be close to 2 （用在反切后頭 表示前兩個字是注音用的反切）見 ...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 限制 : place [impose] restrictions on [to]; astrict; restrict; limit; confine; shut down on [upon]: 限制...
  1. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  2. The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation

    利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。
  3. Large dna molecules are first dissected with restriction enzymes to produce specific fragments.

    首先用限制性內切酶將大的DNA分子切斷，產生出特殊的片段。
  4. Insertional mutagenesis of trichoderma obtained by remi technique

    利用限制性內切酶技術獲得木黴菌插入變異
  5. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  6. Based upon the comparison of cyto b gene sequences in 15 deer species downloaded from genbank, a universal primer set l15774 / hsf21 was used as positive control of the template quality, at the meantime, two species specific primer sets df / dr and cf / cr were desgined to identify red deer ( cervus elaphus ), sika deer ( cervus nippori ) and roe deer ( capreolus capreolus ) from other species. a musk deer ( moschus ) specific primer set wf / mr was designed, siberian musk deer ( afoschus moschiferns ) and forest musk desr ( moschus berezovskii ) could be discriminated when restriction endonuclease rsa i was used to cut the pcr products

    經過對來自genbank中的15種鹿類動物的cytob基因序列的比較，用通用引物l15774和hsf21作為模板的質量控制，設計了特異性引物df dr和cf cr來鑒定馬鹿、梅花鹿、狍；設計了麝類特異性引物mf mr ，用限制性內切酶rsa酶切擴增產物來區分原麝和林麝。
  7. Restriction enzymes and dna probes are used to determine the exact sequence, but it is possible to get a rough estimate by analyzing the frequency of recombination between the alleles of linked genes

    雖然可以利用限制性內切酶和dna探針精確的分析出特定序列，但是在分析連鎖基因等位基因間的重組序列時，會出現不準確的估計。
  8. An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined

    將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上，構建了4個全長基因的分子克隆，分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中兩個轉移到低拷貝載體plg338上，命名為plgd30343 、 plgd70344 。
  9. After the plasmid was assayed for dna sequence, it was transformed into gs115 by electroporation

    利用限制性內切酶分析、 a序列測定在a水平對質粒進行鑒定。
  10. It indicated that the chinese isolates belong to north american group. two pairs of different primers of orf7 were used to identify the genotype of prrsv isolates in china, and then compared with the reference isolates of north american and european serotype and modified live prrsv vaccine. the results further proved that prrsv prevalent in china belongs to b genetype. combining the restriction enzyme digestion patterns obtained from mini, hindi and sactt, we observed 2 distinct rflp patterns

    在此基礎上，擴增各毒株的orf5基因，用mlu ， hinc和sac限制性內切酶切割orf5基因，通過這3種限制性內切酶獲得了各毒株的orf5基因限制性酶切圖譜，經rflp分析表明國內分離毒株與美洲型強毒株有著相同的rflp圖譜，而與疫苗毒的rflp圖譜存在明顯差異，進一步證明國內分離毒株的基因型屬於美洲型的強毒株。
  11. Methods isolates were identified as acinetobacter calcoaceticus by using antibiotic susceptibility test, plasmid profiles, restriction enzyme fingerprinting assay and plasmid elimination method

    方法對我院不動桿菌的感染進行調查，採用藥敏試驗、質粒抽提和限制性核酸內切酶分析法、質粒消除試驗。
  12. Primer - introduced restriction analysis - pcr, pira - pcr

    以引物引入限制性內切酶分析聚合酶鏈反應
  13. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  14. It enables a specific gene to be located on a particular restriction enzyme fragment.

    它就能使專一的基因被定位於特定的限制性內切酶切成的片段上。
  15. Other chromosome elements, telomere and ars, have also been cloned for constructing artificial chromosome. arabidopsis telomere was cloned from pcr products using telomere repeat primers without other template. a 2000bp fragment of ars was released from arabidopsis genomic bac clone t14a4 by claidigestion and subcloned into clai digested pbluescript

    擬南芥的端粒是利用端粒的重復序列進行無模板的pcr擴增得到的；約2000bp的ars片段是從擬南芥的bac克隆t14a4中用限制性內切酶c1a1切下，然後亞克隆到通用載體pbluescript上。
  16. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  17. Two useful restriction endonucleases ( sspl and saci ) were choosed to type the different pathgenic ibdv strains. the result is saci only cleaved cibdv ( 4vaccine strains : bj836, b87, d78, bdc and 6 standard cibdv strains : hel, he2, he3, he4, sd3 / 98, zj1 / 98 ) and vibdv ( american variant - e ) rt - pcr products, whereas products obtained with vvibdv strains ( yl1, yl2, yl5, ylz ) were only cleaved with sspl

    選用具有分型意廬盧人聳2003屆碩十學位論文2義的兩種限制性內切酶（ saci和sspi ）建立的rea ，對5株屬于cibdv的疫苗株和6株標準強毒株；屬于vvibdv的毒株gx 、 yli 、 ylz和ylz等分離毒：屬于葉v的美國變異e株進行酶切分析，結果與前人的研究相符。
  18. Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained

    Pcr產物經回收后，經瓊脂糖凝膠電泳鑒定后，與pcambia1303連接並轉化大腸桿菌dh5a ，陽性重組子經pcr和限制性內切酶酶切圖譜分析，表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。
  19. The restriction map of phzl318 carrying the entire add gene cluster from s. avermitilis nrrl8165 was made. its two subclones phz2104 and phz2105 were introduced into s. lividans mutant zx1

    製作了攜帶完整add基因簇的phz1318的限制內切酶圖譜，根據酶譜進行亞克隆得到phz2104和phz2105 。
  20. To screen the library, defferential screening had been employed. sixty - four clones were randomly picked to perform dot blot

    隨機挑出13個克隆進行限制內切酶分析，結果有10個質粒含有300一600bp的插入片段。
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